GRB2 Antibody (JE49-73)

Western Blot: GRB2 Antibody (JE49-73) [NBP3-32403]

Western Blot: GRB2 Antibody (JE49-73) [NBP3-32403] - Western blot analysis of GRB2 on different lysates with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: A431 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: HCT 116 cell lysate
Lane 6: COS-1 cell lysate
Lane 7: RAW264.7 cell lysate
Lane 8: NIH/3T3 cell lysate
Lane 9: C6 cell lysate
Lane 10: PC-12 cell lysate
Lane 11: Human lung tissue lysate
Lane 12: Mouse testis tissue lysate
Lane 13: Rat testis tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 25 kDa
Observed band size: 25 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32403) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (JE49-73) [NBP3-32403]

Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (JE49-73) [NBP3-32403] - Immunocytochemistry analysis of PC-12 cells labeling GRB2 with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: GRB2 Antibody (JE49-73) [NBP3-32403]

Flow Cytometry: GRB2 Antibody (JE49-73) [NBP3-32403] - Flow cytometric analysis of MCF7 cells labeling GRB2.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32403, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).