hnRNP L Antibody (4D11) - BSA Free

Novus Biologicals | Catalog # NB120-6106

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Amphibian, Bovine, Chicken, Primate

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D11

Format

BSA Free
View all hnRNP L Primary Antibodies »

Product Specifications

Immunogen

Human hnRNP proteins from HeLa cells purified by affinity chromatography on ssDNA agarose.

Reactivity Notes

Newt.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for hnRNP L Antibody (4D11) - BSA Free

Western Blot: hnRNP L Antibody (4D11)BSA Free [NB120-6106]

Western Blot: hnRNP L Antibody (4D11)BSA Free [NB120-6106]

Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - analysis of hnRNP L in HEK whole cell lysate using anti-hnRNP L antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: hnRNP L Antibody (4D11) - BSA Free [NB120-6106]

Immunocytochemistry/ Immunofluorescence: hnRNP L Antibody (4D11) - BSA Free [NB120-6106]

Immunocytochemistry/Immunofluorescence: hnRNP L Antibody (4D11) [NB120-6106] - hnRNP L antibody was tested in HeLa cells at 5 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.
Immunohistochemistry-Frozen: hnRNP L Antibody (4D11) [NB120-6106]
Western Blot: hnRNP L Antibody (4D11)BSA Free [NB120-6106]

Western Blot: hnRNP L Antibody (4D11)BSA Free [NB120-6106]

Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - Western blot for anti-hnRNP-L on HeLa cell extracts
Western Blot: hnRNP L Antibody (4D11)BSA Free [NB120-6106]

Western Blot: hnRNP L Antibody (4D11)BSA Free [NB120-6106]

Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - Whole cell protein from HeLa (lane 1), A431 (lane 2), NIH-3T3 (lane 3) and PC12 (lane 4) was separated by SDS-PAGE and transferred to PVDF membrane. The membrane was probed with Anti-hnRNP L at 1 ug/ml and detected with an HRP conjugated anti-mouse secondary antibody by chemiluminescence.

Applications for hnRNP L Antibody (4D11) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunohistochemistry-Frozen

1:10-1:2000

Immunoprecipitation

1:10-1:500

Simple Western

1:10 - 1:250

Western Blot

1:5000

Reviewed Applications

Read 3 reviews rated 4.7 using NB120-6106 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: hnRNP L

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (HNRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with HNRNP complexes and along with other HNRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of HNRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions.Strucutrally, hnRPL contains 2 segments of approximately 80 amino acids each which are weakly related to each other and to the ribonucleoprotein consensus sequence-type RNA-binding domains of other HNRNP and snRNP proteins.

Alternate Names

FLJ35509, heterogeneous nuclear ribonucleoprotein L, hnRNP L, hnRNP-L, HNRPL

Entrez Gene IDs

3191 (Human); 15388 (Mouse)

Gene Symbol

HNRNPL

UniProt

P14866 (Human), Q8R081 (Mouse)

Additional hnRNP L Products

Product Documents for hnRNP L Antibody (4D11) - BSA Free

Certificate of Analysis

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Product Specific Notices for hnRNP L Antibody (4D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for hnRNP L Antibody (4D11) - BSA Free

Customer Reviews for hnRNP L Antibody (4D11) - BSA Free (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
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1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Rat Primary Motor Neurons
    Species: Rat
    Verified Customer | Posted 10/22/2015
    hnRNP L Antibody (4D11) - BSA Free NB120-6106
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK whole cell lysates
    Species: Human
    Verified Customer | Posted 11/14/2014
    WB for hnRNP-L in HEK cells
    hnRNP L Antibody (4D11) - BSA Free NB120-6106
  • Name: Nadine Bakkar
    Application: Western Blot
    Sample Tested: HEK nuclear lysate
    Species: Human
    Verified Customer | Posted 08/19/2014

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