Human 4-1BB Ligand/TNFSF9 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB22952-100
MAB22952-SP
Detection of 4‑1BB Ligand/TNFSF9 in Human Daudi cell line by Flow Cytometry.
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Human 4-1BB Ligand/TNFSF9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human 4-1BB Ligand/TNFSF9 in direct ELISA.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2357A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived human 4-1BB Ligand/TNFSF9
Arg71-Glu254
Accession # P41273.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of 4‑1BB Ligand/TNFSF9 in Human Daudi cell line by Flow Cytometry. View Larger

Detection of 4‑1BB Ligand/TNFSF9 in Human Daudi cell line by Flow Cytometry. Human Daudi Burkitt lymphoma cell line was stained with Rabbit Anti-Human 4‑1BB Ligand/TNFSF9 Mono­clonal Antibody (Catalog # MAB22952, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-1BB Ligand/TNFSF9

4-1BB Ligand (4-1BBL), also known as CD137L, is a type II transmembrane protein that belongs to the TNF superfamily of molecules and plays an important role in immune response activation (1). The 38 kDa human 4-1BB Ligand consists of an 25 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa extracellular domain (ECD) (2). Within the ECD, human 4-1BB Ligand shares 34% aa sequence identity with mouse and rat 4-1BB Ligand. 4-1BB Ligand is expressed by activated B cells, monocytes, macrophages, dendritic cells (DC), T cells, lymphoma and multiple myeloma cells, hematopoietic stem cells, early myeloid progenitors, neurons, and astrocytes (3-9). A 26 kDa soluble form of 4-1BB Ligand can be released from the surface of activated cells and retains bioactivity (10).
4-1BB Ligand binds to 4-1BB/TNFRSF9/CD137 on activated CD4+ and CD8+ T cells, thymocytes, and NK cells as well as on monocytes, neutrophils, DC, and eosinophils. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4+ and CD8+ T cells (4, 11, 12). T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response (12, 13). 4-1BB Ligand function supports the survival and responsiveness of memory T cells during viral infection (13-15). Reverse signaling through 4-1BB Ligand on monocytes induces the production of inflammatory cytokines (5). On macrophages, 4-1BB Ligand associates in cis with TLR4 and enhances inflammatory cytokine production in response to TLR4 ligation (6). Its expression on early myeloid progenitor cells limits the development of dendritic cells, monocytes, and B cells (9).

References
  1. Wang, C. et al. (2009) Immunol. Rev. 229:192.
  2. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
  3. Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
  4. DeBenedette, M.A. et al. (1997) J. Immunol. 158:551.
  5. Langstein, J. et al. (1998) J. Immunol. 160:2488.
  6. Kang, Y.J. et al. (2007) Nat. Immunol. 8:601.
  7. Baessler, T. et al. (2010) Blood 115:3058.
  8. Gullo, C. et al. (2010) PLoS One 5:e10845.
  9. Lee, S.-W. et al. (2008) Nat. Immunol. 9:917.
  10. Salih, H.R. et al. (2001) J. Immunol. 167:4059.
  11. Wen, T. et al. (2002) J. Immunol. 168:4897.
  12. Cannons, J.L. et al. (2001) J. Immunol. 167:1313.
  13. Bertram, E.M. et al. (2002) J. Immunol. 168:3777.
  14. Bukczynski, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:1291.
  15. Lin, G.H.Y. et al. (2009) J. Immunol. 182:934.
Entrez Gene IDs
8744 (Human); 21950 (Mouse); 353218 (Rat)
Alternate Names
41BB Ligand; 4-1BB Ligand; 4-1BBL; 4-1BB-L; CD137L; homolog of mouse 4-1BB-L; receptor 4-1BB ligand; TNFSF9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand superfamily member 9

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