Human ACE-2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF933
ACE‑2 in Human Kidney.
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Product Details
Citations (3)
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Human ACE-2 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ACE-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human ACE is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ACE-2
Gln18-Ser740
Accession # Q9BYF1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ACE‑2 (Catalog # 933-ZN)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry ACE-2 antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

ACE‑2 in Human Kidney. ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Human ACE-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ACE-2

ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

References
  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower,  M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
Long Name
Angiotensin I Converting Enzyme 2
Entrez Gene IDs
59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)
Alternate Names
ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15

Product Datasheets

Citations for Human ACE-2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Common cardiac medications potently inhibit ACE2 binding to the SARS-CoV-2 Spike, and block virus penetration and infectivity in human lung cells
    Authors: H Caohuy, O Eidelman, T Chen, S Liu, Q Yang, A Bera, NI Walton, TT Wang, HB Pollard
    Scientific Reports, 2021;11(1):22195.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: ELISA Detection
  2. Development of a novel, pan-variant aerosol intervention for COVID-19
    Authors: RH Shoemaker, RA Panettieri, SK Libutti, HS Hochster, NR Watts, PT Wingfield, P Starkl, L Pimenov, R Gawish, A Hladik, S Knapp, D Boring, JM White, Q Lawrence, J Boone, JD Marshall, RL Matthews, BD Cholewa, JW Richig, BT Chen, DL McCormick, R Gugensberg, S Höller, JM Penninger, G Wirnsberge
    bioRxiv : the preprint server for biology, 2021;0(0):.
    Species: Human
    Sample Types: Virus Construct
    Applications: ELISA Detection
  3. Furin cleavage of the SARS coronavirus spike glycoprotein enhances cell-cell fusion but does not affect virion entry.
    Authors: Follis KE, York J, Nunberg JH
    Virology, 2006;350(2):358-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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