Human/Mouse/Rat/Hamster ACE-2 Antibody

Catalog # Availability Size / Price Qty
AF933
AF933-SP
Human/Mouse/Rat/Hamster ACE-2 Antibody in Western Blot
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Product Details
Citations (23)
FAQs
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Reviews (1)

Human/Mouse/Rat/Hamster ACE-2 Antibody Summary

Species Reactivity
Human, Mouse, Rat, Hamster
Specificity
Detects human ACE-2 in direct ELISAs. Detects human, mouse, and rat ACE-2 in Western blots. Detects Hamster ACE-2 in immunohistochemistry. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ACE is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ACE-2
Gln18-Ser740
Accession # Q9BYF1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
3-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ACE‑2 (Catalog # 933-ZN), see our available Western blot detection antibodies
Blockade of Receptor-ligand Interaction
Hoffman, M. et al. (2020) Cell. DOI:10.1016/j.cell.2020.02.052. This application was not tested by R&D Systems.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot View Larger

Detection of Human, Mouse, and Rat ACE‑2 by Western Blot. Western blot shows lysates of human kidney tissue, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with  µg/mL of Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE‑2 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry View Larger

Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF933) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunohistochemistry ACE‑2 in Human Kidney. View Larger

ACE‑2 in Human Kidney. ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry ACE‑2 in Human Kidney. View Larger

ACE‑2 in Human Kidney. ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry View Larger

ACE‑2 in Hamster Lung. ACE‑2 was detected in immersion fixed paraffin-embedded sections of hamster lung using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Human ACE‑2 by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human ACE‑2 by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE-2 at approximately 155 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ACE-2

ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (4, 5). This Human anti-ACE2 antibody (catalog # AF933) was used to block the variant SARS-CoV-2 and ACE2 interaction to elucidate viral transmission and potential therapeutic strategies. (5)

References
  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower,  M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  4. Li, W. et al. (2003) Nature 426:450.
  5. Hoffmann, M. et al. (2020) Cell. DOI: 10.1016/j.cell.2020.02.052.
Long Name
Angiotensin I Converting Enzyme 2
Entrez Gene IDs
59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)
Alternate Names
ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15

Product Datasheets

Citations for Human/Mouse/Rat/Hamster ACE-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays
    Authors: KHD Crawford, R Eguia, AS Dingens, AN Loes, KD Malone, CR Wolf, HY Chu, MA Tortorici, D Veesler, M Murphy, D Pettie, NP King, AB Balazs, JD Bloom
    Viruses, 2020;12(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor.
    Authors: Hoffmann M, Kleine-Weber H, Schroeder S, Kruger N, Herrler T, Erichsen S, Schiergens T, Herrler G, Wu N, Nitsche A, Muller M, Drosten C, Pohlmann S
    Cell, 2020;181(0):1-10.
    Species: Primate - Cercopithecus aethiops (African Green Monkey)
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Protective effects of the Francisella tularensis δpdpC mutant against its virulent parental strain SCHU P9 in Cynomolgus macaques
    Authors: D Tian, A Uda, Y Ami, A Hotta, ES Park, N Nagata, N Iwata-Yosh, A Yamada, K Hirayama, K Miura, Y Koyama, M Azaki, S Morikawa
    Sci Rep, 2019;9(1):9193.
    Species: Rhesus Macaca (Macaque)
    Sample Types: Whole Tissue
  4. Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA
    Authors: E Schrom, M Huber, M Aneja, C Dohmen, D Emrich, J Geiger, G Hasenpusch, A Herrmann-J, V Kretzschma, O Mykhailyk, T Pasewald, P Oak, A Hilgendorf, D Wohlleber, HG Hoymann, D Schaudien, C Plank, C Rudolph, R Kubisch-Do
    Mol Ther Nucleic Acids, 2017;7(0):350-365.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. HTCC: Broad Range Inhibitor of Coronavirus Entry
    Authors: Aleksandra Milewska
    PLoS ONE, 2016;11(6):e0156552.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: IHC
  6. Protein Kinase C-? Mediates Shedding of Angiotensin-Converting Enzyme 2 from Proximal Tubular Cells.
    Authors: Fengxia Xiao, Joseph Zimpelman, Dylan Burger, Christopher Kennedy, Richard L Hébert, Kevin D Burns
    Frontiers in Pharmacology, 2016;0(0):1663-9812.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  7. Surface vimentin is critical for the cell entry of SARS-CoV.
    Authors: Yu Y, Chien S, Chen I, Lai C, Tsay Y, Chang S, Chang M
    J Biomed Sci, 2016;23(1):14.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Whole Cells
    Applications: Neutralization
  8. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein.
    Authors: Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S
    J Virol, 2014;88(2):1293-307.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Characterization of angiotensin-converting enzyme 2 ectodomain shedding from mouse proximal tubular cells.
    Authors: Xiao F, Zimpelmann J, Agaybi S, Gurley S, Puente L, Burns K
    PLoS ONE, 2014;9(1):e85958.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection.
    Authors: Eckerle I, Muller M, Kallies S, Gotthardt D, Drosten C
    Virol J, 2013;10(0):359.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor.
    Authors: Ge, Xing-Yi, Li, Jia-Lu, Yang, Xing-Lou, Chmura, Aleksei, Zhu, Guangjia, Epstein, Jonathan, Mazet, Jonna K, Hu, Ben, Zhang, Wei, Peng, Cheng, Zhang, Yu-Ji, Luo, Chu-Ming, Tan, Bing, Wang, Ning, Zhu, Yan, Crameri, Gary, Zhang, Shu-Yi, Wang, Lin-Fa, Daszak, Peter, Shi, Zheng-Li
    Nature, 2013;503(7477):535-8.
    Species: Bat
    Sample Types: Whole Cells
    Applications: IHC
  12. Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts.
    Authors: Bertram S, Heurich A, Lavender H, Gierer S, Danisch S, Perin P, Lucas JM, Nelson PS, Pohlmann S, Soilleux EJ
    PLoS ONE, 2012;7(4):e35876.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Intrarenal renin angiotensin system revisited: role of megalin-dependent endocytosis along the proximal nephron.
    Authors: Pohl M, Kaminski H, Castrop H, Bader M, Himmerkus N, Bleich M, Bachmann S, Theilig F
    J. Biol. Chem., 2010;285(53):41935-46.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Lipid rafts are involved in SARS-CoV entry into Vero E6 cells.
    Authors: Lu Y, Liu DX, Tam JP
    Biochem. Biophys. Res. Commun., 2008;369(2):344-9.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system.
    Authors: Epelman S, Tang WH, Chen SY, Van Lente F, Francis GS, Sen S
    J. Am. Coll. Cardiol., 2008;52(9):750-4.
    Species: Human
    Sample Types: Plasma
    Applications: Neutralization
  16. Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2.
    Authors: Glende J, Schwegmann-Wessels C, Al-Falah M, Pfefferle S, Qu X, Deng H, Drosten C, Naim HY, Herrler G
    Virology, 2008;381(2):215-21.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Clathrin-dependent entry of severe acute respiratory syndrome coronavirus into target cells expressing ACE2 with the cytoplasmic tail deleted.
    Authors: Inoue Y, Tanaka N, Tanaka Y, Inoue S, Morita K, Zhuang M, Hattori T, Sugamura K
    J. Virol., 2007;81(16):8722-9.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Difference in receptor usage between severe acute respiratory syndrome (SARS) coronavirus and SARS-like coronavirus of bat origin.
    Authors: Ren W, Qu X, Li W, Han Z, Yu M, Zhou P, Zhang SY, Wang LF, Deng H, Shi Z
    J. Virol., 2007;82(4):1899-907.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Synthetic reconstruction of zoonotic and early human severe acute respiratory syndrome coronavirus isolates that produce fatal disease in aged mice.
    Authors: Rockx B, Sheahan T, Donaldson E, Harkema J, Sims A, Heise M, Pickles R, Cameron M, Kelvin D, Baric R
    J. Virol., 2007;81(14):7410-23.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Interferon-gamma and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells.
    Authors: de Lang A, Osterhaus AD, Haagmans BL
    Virology, 2006;353(2):474-81.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Whole Cells
    Applications: ICC
  21. Apical entry and release of severe acute respiratory syndrome-associated coronavirus in polarized Calu-3 lung epithelial cells.
    Authors: Tseng CT, Tseng J, Perrone L, Worthy M, Popov V, Peters CJ
    J. Virol., 2005;79(15):9470-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor.
    Authors: Hofmann H, Geier M, Marzi A, Krumbiegel M, Peipp M, Fey GH, Gramberg T, Pohlmann S
    Biochem. Biophys. Res. Commun., 2004;319(4):1216-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV.
    Authors: Ou X, Liu Y, Lei X, Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang Z, Mu Z, Chen X, Chen J, Hu K, Jin Q, Wang J, Qian Z
    Nat Commun, 0;11(1):1620.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human ACE-2 Antibody
By Anonymous on 08/12/2016
Application: WB Sample Tested: Blood mononuclear cells (PBMCs),Human CD34+ Cells Species: Human