Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2129F
View all Histone H4 Primary Antibodies »

Product Specifications

Immunogen

Human Histone H4 synthetic peptide
Accession # P62805 (H4_Human)

Specificity

Detects human Acetyl Histone H4 when acetylated at Lys5 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Acetyl Histone H4 (Lys5) Antibody

Detection of Human Acetyl Histone H4 (Lys5) antibody by Western Blot.

Detection of Human Acetyl Histone H4 (Lys5) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 10mM Sodium (Na) Butyrate for 24 hours. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Acetyl Histone H4 (Lys5) Monoclonal Antibody (Catalog # MAB9549) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Acetyl Histone H4 (Lys5) at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Acetyl Histone H4 (Lys5) antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Acetyl Histone H4 (Lys5) in HeLa Human Cell Line.

Histone H4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Acetyl Histone H4 (Lys5) Monoclonal Antibody (Catalog # MAB9549) at 0.1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Acetyl Histone H4 (Lys5) Antibody

Application
Recommended Usage

Immunocytochemistry

0.1-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with Sodium (Na) Butyrate

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Histone H4

Histone4 (H4/HIST1H4I; also H4M and HIST4H4) is a 12-15 kDa member of the histone H4 family of molecules. Human histone 4 is 103 amino acids (aa) in length. Full length histone 4 is identical in aa sequence to mouse histone 4. H4 is ubiquitously expressed. Histones are the organizing core of nucleosomes, forming an octamer comprised of four histone dimers. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries, mediating transcription regulation, DNA repair, DNA replication and chromosomal stability. Acetylation of the Histone H4 lysine residue at position 5 (K5) reduces the positive charge on the histones, relaxing the nucleosome; the relaxed state is associated with higher levels of gene transcription. Misregulation of acetylation has been linked to developmental and neurodegenerative disorders including Alzheimer's disease, and cancer. Butyryl-CoA has been shown to induce the K5 acetylation on H4.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4

Entrez Gene IDs

121504 (Human); 319159 (Mouse)

Gene Symbol

H4-16

UniProt

P62805 (H4_Human)

Additional Histone H4 Products

Product Documents for Human Acetyl Histone H4 (Lys5) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Acetyl Histone H4 (Lys5) Antibody

For research use only

Related Research Areas

Epigenetics
Notch Pathway

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail