Human Acetyl Histone H4 (Lys5) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9549-100
MAB9549-SP
Detection of Human Acetyl Histone H4 (Lys5) by Western Blot.
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Human Acetyl Histone H4 (Lys5) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Acetyl Histone H4 when acetylated at Lys5 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2129F
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human Histone H4 synthetic peptide
Accession # P62805 (H4_Human)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunocytochemistry
0.1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Acetyl Histone H4 (Lys5) antibody by Western Blot. View Larger

Detection of Human Acetyl Histone H4 (Lys5) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 10mM Sodium (Na) Butyrate for 24 hours. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Acetyl Histone H4 (Lys5) Monoclonal Antibody (Catalog # MAB9549) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Acetyl Histone H4 (Lys5) at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Acetyl Histone H4 (Lys5) antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Acetyl Histone H4 (Lys5) in HeLa Human Cell Line. Histone H4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Acetyl Histone H4 (Lys5) Monoclonal Antibody (Catalog # MAB9549) at 0.1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Histone H4

Histone4 (H4/HIST1H4I; also H4M and HIST4H4) is a 12-15 kDa member of the histone H4 family of molecules. Human histone 4 is 103 amino acids (aa) in length. Full length histone 4 is identical in aa sequence to mouse histone 4. H4 is ubiquitously expressed. Histones are the organizing core of nucleosomes, forming an octamer comprised of four histone dimers. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries, mediating transcription regulation, DNA repair, DNA replication and chromosomal stability. Acetylation of the Histone H4 lysine residue at position 5 (K5) reduces the positive charge on the histones, relaxing the nucleosome; the relaxed state is associated with higher levels of gene transcription. Misregulation of acetylation has been linked to developmental and neurodegenerative disorders including Alzheimer's disease, and cancer. Butyryl-CoA has been shown to induce the K5 acetylation on H4.

Entrez Gene IDs
121504 (Human); 319159 (Mouse)
Alternate Names
H4; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4F2; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; histone 4, H4; Histone Cluster 1 H4; Histone Cluster 1 H4i; Histone Cluster 4 H4; histone cluster 4, H4; Histone H4; MGC24116

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