Human ADAM28 Antibody Summary
Leu72 & Leu191-Ala623,
Accession # Q0UKQ2
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of ADAM28 in Human PBMC by Flow Cytometry. Human PBMC were stained with (A) Mouse Anti-Human ADAM28 Monoclonal Antibody (Catalog # MAB93252) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
A distintegrin and metalloprotease domain-containing protein 28 (ADAM28), also known as MDC-L, is a member of the M12B peptidase family of enzymes. It is synthesized as an approximately 80-90 kDa glycosylated proprotein that is processed to a mature form later in the secretory pathway (1-3). After the removal of the propeptide that contains a cysteine switch motif, the activated form of ADAM28 consists of a 467 aa extracellular domain (ECD) which contains a peptidase, disintegrin, cysteine-rich and EGF-like domains, followed by a 21 aa transmembrane segment and an 89 aa cytoplasmic domain (3). Alternative splicing generates additional isoforms with a variety of substitutions and deletions in the cysteine-rich and EGF-like domains (1). Within the ECD, human ADAM28 shares 73% aa sequence identity with mouse and rat ADAM28 (3). ADAM28 is a cell surface protein that is involved in a variety of cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis (4-6), and exhibits catalytic activity to insulin-like growth factor binding protein-3 (7). Like many members of the ADAM proteins, the disintegrin domain of ADAM28 interacts with integrin to influence cell adhesion and cell-cell interaction (8). ADAM28 can mediate the adhesion of the Jurkat cells, T-lymphoma cell line, through integrin alpha 4 beta 1(9). R&D Systems in-house testing also indicates that ADAM28 cleaves IGFBP-3.
- Roberts, C.M. et al. (1999) J. Biol. Chem. 274:29251.
- McGinn, O.J. et al. (2011) Cell Biol. Int. 35:1043.
- Howard, L. et al. (2000) Biochem. J. 348:21.
- Mochizuki, S. and Okada Y, (2007) Cancer Sci. 98:621.
- Schlondorff, J. et al. (1999) J. Cell Sci. 112:3603.
- Blobel, C.P. (1997) Cell 90:589.
- Mochizuki S. and Okada Y. (2009) Curr. Pharm. Des. 15:2349.
- Edwards, L. et al. (2008) Molecular Aspects of Medicine 29:258.
- Bridges, L. et al. (2002) J. Biol. Chem. 277:3784.
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