Human ADAM33 Antibody

Catalog # Availability Size / Price Qty
Detection of ADAM33 in A549 Human Cell Line by Flow Cytometry.
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Product Details
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Human ADAM33 Antibody Summary

Species Reactivity
Detects human ADAM33 in direct ELISAs. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse ADAM33 is observed.
Monoclonal Mouse IgG1 Clone # 508101
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human ADAM33
Val30-Leu501 (predicted)
Accession # Q9BZ11
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ADAM33, see our available Western blot detection antibodies
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of ADAM33 antibody in A549 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ADAM33 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human ADAM33 Monoclonal Antibody (Catalog # MAB5565, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM33

ADAM33 is a widely expressed member of the ADAM family of transmembrane proteins. It contains a metalloprotease-like domain, disintegrin cell adhesion domain, and cysteine rich domain. ADAM33 is involved in the maintenance of airway function, and ADAM33 polymorphisms have been associated with asthma susceptibility and bronchial hyperresponsiveness. Within the mature ectodomain, human and mouse ADAM33 share 79% amino acid sequence identity.

Long Name
A Disintegrin and Metalloproteinase Domain 33
Entrez Gene IDs
80332 (Human); 110751 (Mouse)
Alternate Names
a disintegrin and metalloprotease 33; a disintegrin and metalloproteinase domain 33; ADAM 33; ADAM metallopeptidase domain 33; ADAM33; C20orf153; chromosome 20 open reading frame 153; disintegrin and metalloproteinase domain-containing protein 33; disintegrin and reprolysin metalloproteinase family protein; dJ964F7.1; DKFZp434K0521; EC 3.4.24.-; FLJ35308; FLJ36751; MGC149823; MGC71889

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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