Human ALK-7 Antibody

Catalog # Availability Size / Price Qty
MAB7749
MAB7749-SP
ALK‑7 in Human Pancreas.
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Human ALK-7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ALK‑7 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant rat ALK7 is observed and no cross‑reactivity with recombinant mouse Activin RIA/ALK2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 810510
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ALK‑7
Leu26-Glu113
Accession # Q8NER5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry ALK-7 antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

ALK‑7 in Human Pancreas. ALK-7 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human ALK-7 Monoclonal Antibody (Catalog # MAB7749) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALK-7

Activin receptor-like kinase 7 (ALK-7), also known as Activin R1C (gene name ACVR1C), is a glycosylated 58 kDa type I receptor in the superfamily of TGF-beta serine/threonine kinase receptors. It associates with type II receptors to form a signaling complex that responds to the ligands Activin AB, and Activin B, GDF3, and Nodal. ALK-7 plays a role in regulating energy balance by inhibiting insulin secretion and inducing pancreatic beta cell apoptosis. It is expressed in adipose tissue but downregulated in obesity. ALK-7 is also expressed in pituitary gonadotropic cells and in pre-eclamptic placenta. It induces the apoptosis of trophoblasts as well as ovarian granulosa and epithelial cells. Within the extracellular domain, human ALK-7 shares 95% and 91% amino acid (aa) sequence identity with mouse and rat ALK-7, respectively. Alternate splicing of human ALK-7 generates additional isoforms with either a 50 aa N-terminal truncation or with deletions of 79 aa or 157 aa that encompass the transmembrane segment.

Long Name
Activin Receptor-like Kinase 7
Entrez Gene IDs
130399 (Human); 245921 (Rat)
Alternate Names
activin A receptor, type IC; Activin receptor type IC; activin receptor type-1C; Activin receptor-like kinase 7; Activin RIC; ACTR-IC; ACVR1C; ACVRLK7; ALK7; ALK-7; ALK7ALK-7; EC 2.7.11; EC 2.7.11.30

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human ALK-7 Antibody

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Human ALK-7 Antibody
By Anonymous on 12/31/2021
Application: IHC Sample Tested: Pancreas tissue Species: Human