Human alpha-Defensin 1 Antibody

R&D Systems | Catalog # AF8198

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, ELISA

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all alpha-Defensin 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human alpha ‑Defensin 1
Ala65-Cys94
Accession # P59665

Specificity

Detects human  alpha ‑Defensin 1 in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human alpha-Defensin 1 Antibody

a-Defensin 1 antibody in Human Small Intestine by Immunohistochemistry (IHC-P).

alpha ‑Defensin 1 in Human Small Intestine.

a-Defensin 1 was detected in formalin fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human a-Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to lamina propria in intestinal villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Human a‑Defensin 1 Antibody in ELISA Standard Curve.

Human alpha ‑Defensin 1 ELISA Standard Curve.

Recombinant Human a-Defensin 1 protein was serially diluted 2-fold and captured by Mouse Anti-Human a-Defensin 1 Monoclonal Antibody (Catalog # MAB8198) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human a-Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human alpha-Defensin 1 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human alpha ‑Defensin 1 Monoclonal Antibody (Catalog # MAB8198).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human alpha-Defensin 1 DuoSet ELISA Kit (Catalog # DY8198-05) for convenient development of a sandwich ELISA.

Immunohistochemistry

5-15 µg/mL
Sample: Formalin fixed paraffin-embedded sections of human small intestine

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha-Defensin 1

Human Defensin alpha 1 (DEFA1) is 94 amino acids (aa) in length and is cleaved into the following chains: HP 1-56 (aa 39-94), Neutrophil defensin 1 (aa 65-94) and Neutrophil defensin 2 ( aa 66-94). Defensins are secreted peptides that kill microbes by permeabilizing their plasma membrane. DEFA1 has antimicrobial activity against Gram-negative and Gram-positive bacteria. ADP-ribosylation reduces cytotoxic and antibacterial activities, it is also known to enhance IL8 production. Phosphorylation at Tyr-85 is reported in several cancer cell lines and interferes with ADP-ribosylation of DEFA1.

Alternate Names

alphaDefensin 1, DEF1, DEFA1, HNP-1, HP1, MRS

Entrez Gene IDs

1667 (Human); 13216 (Mouse)

Gene Symbol

DEFA1

UniProt

P59665

Additional alpha-Defensin 1 Products

Product Documents for Human alpha-Defensin 1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human alpha-Defensin 1 Antibody

For research use only

Citations for Human alpha-Defensin 1 Antibody

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Protocols

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