Human alpha-Defensin 1 Antibody

Catalog # Availability Size / Price Qty
Human alpha-Defensin 1 Antibody in Immunohistochemistry
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Product Details
Supplemental Products

Human alpha-Defensin 1 Antibody Summary

Species Reactivity
Detects human  alpha ‑Defensin 1 in direct ELISAs.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human alpha ‑Defensin 1
Accession # P59665
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human alpha ‑Defensin 1 Monoclonal Antibody (Catalog # MAB8198).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human alpha-Defensin 1 DuoSet ELISA Kit (Catalog # DY8198-05) for convenient development of a sandwich ELISA.

5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry alpha ‑Defensin 1 in Human Small Intestine. View Larger

alpha ‑Defensin 1 in Human Small Intestine. alpha ‑Defensin 1 was detected in formalin fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human alpha ‑Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to lamina propria in intestinal villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Human alpha ‑Defensin 1 ELISA Standard Curve. Recombinant Human alpha ‑Defensin 1 protein was serially diluted 2-fold and captured by Mouse Anti-Human alpha ‑Defensin 1 Monoclonal Antibody (Catalog # MAB8198) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human alpha ‑Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Defensin 1

Human Defensin alpha 1 (DEFA1) is 94 amino acids (aa) in length and is cleaved into the following chains: HP 1-56 (aa 39-94), Neutrophil defensin 1 (aa 65-94) and Neutrophil defensin 2 ( aa 66-94). Defensins are secreted peptides that kill microbes by permeabilizing their plasma membrane. DEFA1 has antimicrobial activity against Gram-negative and Gram-positive bacteria. ADP-ribosylation reduces cytotoxic and antibacterial activities, it is also known to enhance IL8 production. Phosphorylation at Tyr-85 is reported in several cancer cell lines and interferes with ADP-ribosylation of DEFA1.

Entrez Gene IDs
1667 (Human); 13216 (Mouse)
Alternate Names
alpha 2; alphaDefensin 1; alpha-Defensin 1; DEF1; DEF1DEFA1B; DEFA1; DEFA2; defensin, alpha 1, myeloid-related sequence; defensin, alpha 1HP-1; HNP-1; HNP-1HP1; HP1; MRS; MRSMGC138393; myeloid-related sequence; neutrophil defensin 1

Product Datasheets


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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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