Human alpha-Defensin 1 Antibody Summary
Accession # P59665
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human alpha ‑Defensin 1 Monoclonal Antibody (Catalog # MAB8198).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human alpha-Defensin 1 DuoSet ELISA Kit (Catalog # DY8198-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
alpha ‑Defensin 1 in Human Small Intestine. alpha ‑Defensin 1 was detected in formalin fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human alpha ‑Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to lamina propria in intestinal villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Human alpha ‑Defensin 1 ELISA Standard Curve. Recombinant Human alpha ‑Defensin 1 protein was serially diluted 2-fold and captured by Mouse Anti-Human alpha ‑Defensin 1 Monoclonal Antibody (Catalog # MAB8198) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human alpha ‑Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Defensin 1
Human Defensin alpha 1 (DEFA1) is 94 amino acids (aa) in length and is cleaved into the following chains: HP 1-56 (aa 39-94), Neutrophil defensin 1 (aa 65-94) and Neutrophil defensin 2 ( aa 66-94). Defensins are secreted peptides that kill microbes by permeabilizing their plasma membrane. DEFA1 has antimicrobial activity against Gram-negative and Gram-positive bacteria. ADP-ribosylation reduces cytotoxic and antibacterial activities, it is also known to enhance IL8 production. Phosphorylation at Tyr-85 is reported in several cancer cell lines and interferes with ADP-ribosylation of DEFA1.
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Cell and Tissue Staining Kits
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