Human alpha-Defensin 1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8198-100
MAB8198-SP
Human  alpha ‑Defensin 1 ELISA Standard Curve.
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Human alpha-Defensin 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha ‑Defensin 1 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 867919R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human alpha ‑Defensin 1
Ala65-Cys94
Accession # P59665
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human alpha ‑Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human alpha-Defensin 1 DuoSet ELISA Kit (Catalog # DY8198-05) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human alpha ‑Defensin 1 ELISA Standard Curve. Recombinant Human a-Defensin 1 protein was serially diluted 2-fold and captured by Mouse Anti-Human a-Defensin 1 Monoclonal Antibody (Catalog # MAB8198) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human a-Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Defensin 1

Human Defensin alpha 1 (DEFA1) is 94 amino acids (aa) in length and is cleaved into the following chains: HP 1-56 (aa 39-94), Neutrophil defensin 1 (aa 65-94) and Neutrophil defensin 2 ( aa 66-94). Defensins are secreted peptides that kill microbes by permeabilizing their plasma membrane. DEFA1 has antimicrobial activity against Gram-negative and Gram-positive bacteria. ADP-ribosylation reduces cytotoxic and antibacterial activities, it is also known to enhance IL8 production. Phosphorylation at Tyr-85 is reported in several cancer cell lines and interferes with ADP-ribosylation of DEFA1.

Entrez Gene IDs
1667 (Human); 13216 (Mouse)
Alternate Names
alpha 2; alphaDefensin 1; alpha-Defensin 1; DEF1; DEF1DEFA1B; DEFA1; DEFA2; defensin, alpha 1, myeloid-related sequence; defensin, alpha 1HP-1; HNP-1; HNP-1HP1; HP1; MRS; MRSMGC138393; myeloid-related sequence; neutrophil defensin 1

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