Human alpha-Smooth Muscle Actin Alexa Fluor® 405-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of alpha ‑Smooth Muscle Actin in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cell (PBMCs) were stained with Mouse Anti-Human a-Smooth Muscle Actin Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # IC1420V, filled histogram) or isotype control antibody (Catalog # IC003V, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: alpha-Smooth Muscle Actin
alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
- Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
- Oishi, K. et al. (2002) J. Physiol. 540:139.
Product Specific Notices
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Cells were intracellularly stained for SMA. Negative control is overlay.