Human alpha -Smooth Muscle Actin Alexa Fluor® 405-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human  alpha -Smooth Muscle Actin in flow cytometry.
  • Source
    Monoclonal Mouse IgG2A Clone # 1A4
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 405
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of alpha ‑Smooth Muscle Actin in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cell (PBMCs) were stained with Mouse Anti-Human alpha ‑Smooth Muscle Actin Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # IC1420V, filled histogram) or isotype control antibody (Catalog # IC003V, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: alpha-Smooth Muscle Actin
alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
  • References:
    1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
    2. Oishi, K. et al. (2002) J. Physiol. 540:139.
  • Long Name:
    Actin, Alpha 2, Smooth Muscle, Aorta
  • Entrez Gene IDs:
    59 (Human); 11475 (Mouse); 81633 (Rat)
  • Alternate Names:
    AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Alexa Fluor® 405-conjugated Isotype Control

Ctrl IC003V
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 1
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