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- Species ReactivityHuman
- SpecificityDetects human alpha -Smooth Muscle Actin.
- SourceMonoclonal Mouse IgG2A Clone # 1A4
- PurificationProtein A or G purified from hybridoma culture supernatant
- Immunogenalpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide
- FormulationSupplied in a saline solution containing BSA and Sodium Azide.
- LabelAlexa Fluor 750
Applications
-
Recommended
ConcentrationSample
-
Intracellular Staining by Flow Cytometry5 µL/106 cellsSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
|
Intracellular Staining by Flow Cytometry
|
Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
Preparation and Storage
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageProtect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: alpha-Smooth Muscle Actin
alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
- Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
- Oishi, K. et al. (2002) J. Physiol. 540:139.
- Long Name:Actin, Alpha 2, Smooth Muscle, Aorta
- Entrez Gene IDs:59 (Human); 11475 (Mouse); 81633 (Rat)
- Alternate Names:AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin
Related Research Areas
Isotype Controls
Description
Application
Cat#
Citations
Images
Mouse IgG2A Alexa Fluor® 750-conjugated Isotype Control
Ctrl IC003SView Sizes & Prices
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|---|---|---|---|---|
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Staining Reagents
Description
Application
Cat#
Citations
Images
Flow Cytometry Permeabilization/Wash Buffer I (1X)
Flow FC005 5View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
|---|---|---|---|---|
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Flow Cytometry Fixation Buffer (1X)
Flow FC004 3View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
|---|---|---|---|---|
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Flow Cytometry Fixation & Permeabilization Buffer Kit I
Flow FC009 3View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
|---|---|---|---|---|
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)
Flow FC007 2View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
|---|---|---|---|---|
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Calculators
Reconstitution Calculator
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
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