Human alpha-Smooth Muscle Actin Alexa Fluor® 750-conjugated Antibody
Human alpha-Smooth Muscle Actin Alexa Fluor® 750-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
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Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: alpha-Smooth Muscle Actin
alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
- Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
- Oishi, K. et al. (2002) J. Physiol. 540:139.
Product Datasheets
FAQs
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Staining Reagents
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