Human alpha-Smooth Muscle Actin Alexa Fluor® 750-conjugated Antibody

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Human alpha-Smooth Muscle Actin Alexa Fluor® 750-conjugated Antibody in Data
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Product Details
Citations (1)
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Human alpha-Smooth Muscle Actin Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Detects human  alpha -Smooth Muscle Actin.
Monoclonal Mouse IgG2A Clone # 1A4
Protein A or G purified from hybridoma culture supernatant
alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)


Recommended Concentration
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of a-Smooth Muscle Actin antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: alpha-Smooth Muscle Actin

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

  1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
  2. Oishi, K. et al. (2002) J. Physiol. 540:139.
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Entrez Gene IDs
59 (Human); 11475 (Mouse); 81633 (Rat)
Alternate Names
AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin

Product Datasheets

Citation for Human alpha-Smooth Muscle Actin Alexa Fluor® 750-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. ApoC-III is a novel inducer of calcification in human aortic valves
    Authors: F Schlotter, RCC de Freitas, MA Rogers, MC Blaser, PJ Wu, H Higashi, A Halu, F Iqbal, AB Andraski, CN Rodia, S Kuraoka, JR Wen, M Creager, T Pham, JD Hutcheson, SC Body, AB Kohan, FM Sacks, M Aikawa, SA Singh, E Aikawa
    The Journal of Biological Chemistry, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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