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Human alpha-Smooth Muscle Actin Alexa Fluor® 750-conjugated Antibody

IC1420S Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC1420S-025
Human alpha-Smooth Muscle Actin Alexa Fluor® 750-conjugated Antibody in Intracellular Staining by Flow Cytometry
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha-Smooth Muscle Actin Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human  alpha -Smooth Muscle Actin.
Source
Monoclonal Mouse IgG2A Clone # 1A4
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. View Larger

Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: alpha-Smooth Muscle Actin

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

References
  1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
  2. Oishi, K. et al. (2002) J. Physiol. 540:139.
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Entrez Gene IDs
59 (Human); 11475 (Mouse); 81633 (Rat)
Alternate Names
AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin

Product Datasheets

Product Datasheet
COA
SDS

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Alexa Fluor® 750-conjugated Isotype Control

IC003S
Ctrl

Staining Reagents

Flow Cytometry Fixation/Permeabilization Buffer I (1X)

FC007
4 Citations
1 Review
Flow

Flow Cytometry Fixation Buffer (1X)

FC004
7 Citations
3 Reviews
Flow

Flow Cytometry Permeabilization/Wash Buffer I (1X)

FC005
11 Citations
2 Reviews
Flow

Flow Cytometry Fixation & Permeabilization Buffer Kit I

FC009
8 Citations
1 Review
Flow

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