Human alpha -Smooth Muscle Actin Alexa Fluor® 750-conjugated Antibody

• Species Reactivity
  Human
• Specificity
  Detects human  alpha -Smooth Muscle Actin.
• Source
  Monoclonal Mouse IgG_2A Clone # 1A4
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide
• Formulation
  Supplied in a saline solution containing BSA and Sodium Azide.
• Label
  Alexa Fluor 750

• Intracellular Staining by Flow Cytometry
  5 µL/10⁶ cells
  See below

Intracellular Staining by Flow Cytometry click image to view larger

Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Shipping
  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
• Stability & Storage
  Protect from light. Do not freeze.

  • 12 months from date of receipt, 2 to 8 °C as supplied.

• References:
  1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
  2. Oishi, K. et al. (2002) J. Physiol. 540:139.

• Long Name:
  Actin, Alpha 2, Smooth Muscle, Aorta

• Entrez Gene IDs:
  59 (Human); 11475 (Mouse); 81633 (Rat)

• Alternate Names:
  AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin

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