Human alpha-Smooth Muscle Actin Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC1420G
Detection of  alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry.
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Citations (8)
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Human alpha-Smooth Muscle Actin Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human  alpha -Smooth Muscle Actin.
Source
Monoclonal Mouse IgG2A Clone # 1A4
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of a-Smooth Muscle Actin antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human a-Smooth Muscle Actin Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC1420G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: alpha-Smooth Muscle Actin

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

References
  1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
  2. Oishi, K. et al. (2002) J. Physiol. 540:139.
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Entrez Gene IDs
59 (Human); 11475 (Mouse); 81633 (Rat)
Alternate Names
AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human alpha-Smooth Muscle Actin Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Toll-like receptor 5 tunes hepatic and pancreatic stellate cells activation
    Authors: Pietro Di Fazio, Sophia Mielke, Isabell T Böhm, Malte Buchholz, Sami Matrood, Detlef Schuppan et al.
    BMJ Open Gastroenterology
  2. BDK inhibition acts as a catabolic switch to mimic fasting and improve metabolism in mice
    Authors: E Bollinger, M Peloquin, J Libera, B Albuquerqu, E Pashos, A Shipstone, A Hadjipanay, Z Sun, G Xing, M Clasquin, JC Stansfield, B Tierney, S Gernhardt, CP Siddall, T Greizer, FJ Geoly, SR Vargas, LC Gao, G Williams, M Marshall, A Rosado, C Steppan, KJ Filipski, BB Zhang, RA Miller, RJ Roth Flach
    Molecular Metabolism, 2022-10-08;66(0):101611.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. ACC inhibitor alone or co-administered with a DGAT2 inhibitor in patients with non-alcoholic fatty liver disease: two parallel, placebo-controlled, randomized phase 2a trials
    Authors: RA Calle, NB Amin, S Carvajal-G, TT Ross, A Bergman, S Aggarwal, C Crowley, A Rinaldi, J Mancuso, N Aggarwal, V Somayaji, M Inglot, TA Tuthill, K Kou, M Boucher, G Tesz, R Dullea, KK Bence, AM Kim, JA Pfefferkor, WP Esler
    Nature Medicine, 2021-10-11;27(10):1836-1848.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  4. Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA
    Authors: H Steinle, M Weber, A Behring, U Mau-Holzma, C Schlensak, HP Wendel, M Avci-Adali
    Stem Cells Int, 2019-06-19;2019(0):7641767.
    Species: Human
    Sample Types:
    Applications: Flow Cytometry
  5. Generation of iPSCs from Jaw Periosteal Cells Using Self-Replicating RNA
    Authors: F Umrath, H Steinle, M Weber, HP Wendel, S Reinert, D Alexander, M Avci-Adali
    Int J Mol Sci, 2019-04-03;20(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Growth differentiation factor 15 contributes to cancer-associated fibroblasts-mediated chemo-protection of AML cells
    J Exp Clin Cancer Res, 2016-09-19;35(1):147.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network.
    Authors: Kim J, Chung M, Kim S, Jo D, Kim J, Jeon N
    PLoS ONE, 2015-07-23;10(7):e0133880.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. G-CSF and G-CSFR are highly expressed in human gastric and colon cancers and promote carcinoma cell proliferation and migration.
    Authors: Morris K, Khan H, Ahmad A, Weston L, Nofchissey R, Pinchuk I, Beswick E
    Br J Cancer, 2014-01-21;110(5):1211-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human alpha -Smooth Muscle Actin Alexa Fluor® 488-conjugated Antibody
By Anonymous on 11/03/2017
Application: Flow Sample Tested: Bladder tissue Species: Mouse