Human alpha -Smooth Muscle Actin Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of  alpha ‑Smooth Muscle Actin in Human Monocytes by Flow Cytometry.
1 Image
Product Details
Citations (4)
Supplemental Products

Human alpha -Smooth Muscle Actin Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Detects human  alpha -Smooth Muscle Actin.
Monoclonal Mouse IgG2A Clone # 1A4
Protein A or G purified from hybridoma culture supernatant
alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)


Recommended Concentration
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of a-Smooth Muscle Actin antibody in Human Monocytes antibody by Flow Cytometry. View Larger

Detection of alpha ‑Smooth Muscle Actin in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human a-Smooth Muscle Actin Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC1420N, filled histogram) or isotype control antibody (Catalog # IC003N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: alpha-Smooth Muscle Actin

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

  1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
  2. Oishi, K. et al. (2002) J. Physiol. 540:139.
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Entrez Gene IDs
59 (Human); 11475 (Mouse); 81633 (Rat)
Alternate Names
AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin

Product Datasheets

You must select a language.


Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human alpha -Smooth Muscle Actin Alexa Fluor® 700-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. A vasculature niche orchestrates stromal cell phenotype through PDGF signaling: Importance in human fibrotic disease
    Authors: TB Layton, L Williams, N Yang, M Zhang, C Lee, M Feldmann, G Trujillo, D Furniss, J Nanchahal
    Proceedings of the National Academy of Sciences of the United States of America, 2022-03-23;119(13):e2120336119.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Cellular census of human fibrosis defines functionally distinct stromal cell types and states
    Authors: TB Layton, L Williams, F McCann, M Zhang, M Fritzsche, H Colin-York, M Cabrita, MTH Ng, M Feldmann, SN Sansom, D Furniss, W Xie, J Nanchahal
    Nat Commun, 2020-06-02;11(1):2768.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Identification of TNFR2 and IL-33 as therapeutic targets in localized fibrosis
    Authors: D Izadi, TB Layton, L Williams, F McCann, M Cabrita, AI Espirito S, W Xie, M Fritzsche, H Colin-York, M Feldmann, KS Midwood, J Nanchahal
    Sci Adv, 2019-12-04;5(12):eaay0370.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Deciphering the stromal and hematopoietic cell network of the adventitia from non-aneurysmal and aneurysmal human aorta.
    Authors: Dutertre C, Clement M, Morvan M, Schakel K, Castier Y, Alsac J, Michel J, Nicoletti A
    PLoS ONE, 2014-02-27;9(2):e89983.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human alpha -Smooth Muscle Actin Alexa Fluor® 700-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human alpha -Smooth Muscle Actin Alexa Fluor® 700-conjugated Antibody and earn rewards!

Have you used Human alpha -Smooth Muscle Actin Alexa Fluor® 700-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review