Detection of alpha ‑Smooth Muscle Actin in Human Monocytes by Flow Cytometry.
Human peripheral blood monocytes were stained with Mouse Anti-Human alpha ‑Smooth Muscle Actin Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC1420N, filled histogram) or isotype control antibody (Catalog # IC003N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: alpha-Smooth Muscle Actin
alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
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