Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Catalog # Availability Size / Price Qty
MAB1420
MAB1420-SP
Detection of Human  alpha ‑Smooth Muscle Actin by Western Blot.
8 Images
Product Details
Citations (80)
FAQs
Supplemental Products
Reviews (8)

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human  alpha -Smooth Muscle Actin.
Source
Monoclonal Mouse IgG2A Clone # 1A4
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Peptide corresponding to residues EEEDSTALVC of the N-term of human alpha -smooth muscle actin (1)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Western Blot
0.1-0.25 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Dual RNAscope ISH-IHC
0.5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human a-Smooth Muscle Actin antibody by Western Blot. View Larger

Detection of Human alpha ‑Smooth Muscle Actin by Western Blot. Western blot shows lysates of human heart tissue and iBJ6 human induced pluripotent stem cell line undifferentiated or cardiac differentiated. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/ Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Smooth Muscle Actin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse and Rat a-Smooth Muscle Actin antibody by Western Blot. View Larger

Detection of Mouse and Rat alpha ‑Smooth Muscle Actin by Western Blot. Western blot shows lysates of mouse colon tissue, C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, and rat colon tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Smooth Muscle Actin at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry a-Smooth Muscle Actin antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

alpha ‑Smooth Muscle Actin in BG01V Human Embryonic Stem Cells. a-Smooth Muscle Actin was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiomyocytes using Mouse Anti-Human/Mouse/ Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry a-Smooth Muscle Actin antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

alpha -Smooth Muscle Actin in Human Breast Cancer Tissue. a-Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/ Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry a-Smooth Muscle Actin antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

alpha ‑Smooth Muscle Actin in Human Heart. a-Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Intracellular Staining by Flow Cytometry Detection of a-Smooth Muscle Actin antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human/Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Simple Western Detection of Human a-Smooth Muscle Actin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human alpha ‑Smooth Muscle Actin by Simple WesternTM. Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyocytes (+), loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Smooth Muscle Actin at approximately 49 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Dual RNAscope ISH-IHC Compatible alpha-Smooth Muscle Actin antibody in Human Breast Cancer Tissue Using Dual RNAscope<sup>®</sup>ISH and IHC. View Larger

alpha-Smooth Muscle Actin in Human Breast Cancer Tissue Using Dual RNAscope®ISH and IHC. alpha-Smooth Muscle Actin mRNA was detected (top image) in formalin-fixed paraffin-embedded tissue sections of human breast cancer tissue probed with ACD RNAScope® Probe (Catalog # 444771-C2) and stained using ACD RNAscope® 2.5 HD Duplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section (bottom image) was processed for immunohistochemistry using R&D Systems Mouse Anti-Human/Mouse/Rat alpha-Smooth Muscle Actin Monoclonal Antibody (Catalog# MAB1420) at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001) and DAB chromogen (brown). Tissues were counterstained with hematoxylin (blue).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Smooth Muscle Actin

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

References
  1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
  2. Oishi, K. et al. (2002) J. Physiol. 540:139.
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Entrez Gene IDs
59 (Human); 11475 (Mouse); 81633 (Rat)
Alternate Names
AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin

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Citations for Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

80 Citations: Showing 1 - 10
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  1. Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment
    Authors: D Zheng, J Zhou, L Qian, X Liu, C Chang, S Tang, H Zhang, S Zhou
    Bioactive materials, 2023;22(0):567-587.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Switching in the expression pattern of actin isoforms marks the onset of contractility and distinct mechanodynamic behavior during cardiomyocyte differentiation
    Authors: RH Pires, TH Dau, E Manu, N Shree, O Otto
    Physiological Reports, 2022;10(3):e15171.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. CRISPR/Cas9-engineered human ES cells harboring heterozygous and homozygous c-KIT knockout
    Authors: MAS de Toledo, X Fu, F Kluge, K Götz, S Schmitz, P Wanek, HM Schüler, K Pannen, N Chatain, S Koschmiede, TH Brümmendor, M Zenke
    Stem Cell Research, 2022;60(0):102732.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis
    Authors: SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn
    International Journal of Molecular Sciences, 2022;23(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Dermal Papilla Cell-Derived Extracellular Vesicles Increase Hair Inductive Gene Expression in Adipose Stem Cells via beta-Catenin Activation
    Authors: T Kazi, A Nagata, T Nakagawa, T Matsuzaki, S Inui
    Cells, 2022;11(2):.
    Species: Mouse
    Sample Types: Spheroids
    Applications: ICC
  6. Synergistic effects of silver nanoparticles and cisplatin in combating inflammation and hyperplasia of airway stents
    Authors: Z Li, C Tian, D Jiao, J Li, Y Li, X Zhou, H Zhao, Y Zhao, X Han
    Bioactive materials, 2022;9(0):266-280.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  7. Generation of a human induced pluripotent stem cell line derived from a patient with dilated cardiomyopathy carrying LMNA nonsense mutation
    Authors: Y Shimoda, N Murakoshi, H Mori, D Xu, K Tajiri, Y Hemmi, I Sato, M Noguchi, Y Nakamura, Y Hayashi, M Ieda
    Stem Cell Research, 2022;62(0):102793.
    Species: Human
    Sample Types: Spheroid
    Applications: ICC
  8. Histochemical examination of blood vessels in murine femora with intermittent PTH administration
    Authors: H Maruoka, S Zhao, H Yoshino, M Abe, T Yamamoto, H Hongo, M Haraguchi-, A Nasoori, H Ishizu, Y Nakajima, M Omaki, T Shimizu, N Iwasaki, PH Luiz de Fr, M Li, T Hasegawa
    Journal of oral biosciences, 2022;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Signature of the vascular tumor microenvironment as a marker of the therapeutic response to doxorubicin in a preclinical model of osteosarcoma
    Authors: V Crenn, J Amiaud, A Gomez-Brou, V Potiron, F Gouin, P Rosset, LL Nail, L Vidal, H Bertin, R Brion, G Tran, F Verrecchia, I Corre, F Redini
    American journal of cancer research, 2022;12(4):1843-1854.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. AAV2-mediated gene therapy for Bietti crystalline dystrophy provides functional CYP4V2 in multiple relevant cell models
    Authors: JH Wang, GE Lidgerwood, M Daniszewsk, ML Hu, GE Roberts, RCB Wong, SSC Hung, ME McClements, AW Hewitt, A Pébay, DG Hickey, TL Edwards
    Oncogene, 2022;12(1):9525.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Alcohol and HIV-Derived Hepatocyte Apoptotic Bodies Induce Hepatic Stellate Cell Activation
    Authors: M New-Aaron, RS Dagur, SS Koganti, M Ganesan, W Wang, E Makarov, M Ogunnaike, KK Kharbanda, LY Poluektova, NA Osna
    Biology, 2022;11(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient
    Authors: J Lotila, T Hyvärinen, H Skottman, L Airas, S Narkilahti, S Hagman
    Oncogene, 2022;63(0):102865.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. A vasculature niche orchestrates stromal cell phenotype through PDGF signaling: Importance in human fibrotic disease
    Authors: TB Layton, L Williams, N Yang, M Zhang, C Lee, M Feldmann, G Trujillo, D Furniss, J Nanchahal
    Proceedings of the National Academy of Sciences of the United States of America, 2022;119(13):e2120336119.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  14. Generation of three induced pluripotent stem cell lines, SCVIi003-A, SCVIi004-A, SCVIi005-A, from patients with ARVD/C caused by heterozygous mutations in the PKP2 gene
    Authors: JWS Jahng, KE Black, L Liu, HR Bae, M Perez, EA Ashley, K Sallam, JC Wu
    Stem Cell Research, 2021;53(0):102284.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. A non-invasive method to generate induced pluripotent stem cells from primate urine
    Authors: J Geuder, LE Wange, A Janjic, J Radmer, P Janssen, JW Bagnoli, S Müller, A Kaul, M Ohnuki, W Enard
    Scientific Reports, 2021;11(1):3516.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Cohort Analysis of ADAM8 Expression in the PDAC Tumor Stroma
    Authors: C Jaworek, Y Verel-Yilm, S Driesch, S Ostgathe, L Cook, S Wagner, DK Bartsch, EP Slater, JW Bartsch
    Journal of personalized medicine, 2021;11(2):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. Cancer-associated fibroblast-derived SDF-1 induces epithelial-mesenchymal transition of lung adenocarcinoma via CXCR4/&beta-catenin/PPAR&delta signalling
    Authors: Y Wang, W Lan, M Xu, J Song, J Mao, C Li, X Du, Y Jiang, E Li, R Zhang, Q Wang
    Cell Death & Disease, 2021;12(2):214.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Generation of an induced pluripotent stem cell line GZHMCi008-A derived from a patient with SRY-positive 46,XX testicular disorder of sex development
    Authors: Y Luo, Y Chen, M Zhang, X Ma, D Zhu, Y Chen
    Stem Cell Research, 2021;57(0):102583.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction)
    Authors: K Hira, P Sharma, A Mahale, O Prakash Ku, A Sajeli Beg
    International immunopharmacology, 2021;103(0):108494.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9
    Authors: MR Vitale, JEM Zöller, C Jansch, A Janz, F Edenhofer, E Klopocki, D van den Ho, T Vanmierlo, O Rivero, N Nadif Kasr, GC Ziegler, KP Lesch
    Stem Cell Research, 2021;51(0):102169.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. CD8+ T-cell plasticity regulates vascular regeneration in type-2 diabetes
    Authors: C Liang, KY Yang, VW Chan, X Li, THW Fung, Y Wu, XY Tian, Y Huang, L Qin, JYW Lau, KO Lui
    Theranostics, 2020;10(9):4217-4232.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. SB772077B (SB77) Alleviated the Aqueous Outflow Resistance Mediated by Cyclic Mechanical Stress in Perfused Human Cadaveric Eyes
    Authors: S Ashwinbala, R Haribalaga, S Krishnadas, V Muthukkaru, S Senthilkum
    Sci Rep, 2020;10(1):10202.
    Species: Human
    Sample Types: Whole Tissues
    Applications: IHC
  23. Acetyl-CoA Carboxylase inhibition improves multiple dimensions of NASH pathogenesis in model systems
    Authors: TT Ross, C Crowley, KL Kelly, A Rinaldi, DA Beebe, MP Lech, RV Martinez, S Carvajal-G, M Boucher, D Hirenallur, J Morin, AC Opsahl, SR Vargas, KK Bence, JA Pfefferkor, WP Esler
    Cell Mol Gastroenterol Hepatol, 2020;0(0):.
    Species: Rat
    Sample Types: Whole Tissues
    Applications: IHC
  24. Reconstruction of the human blood-brain barrier in vitro reveals a pathogenic mechanism of APOE4 in pericytes
    Authors: JW Blanchard, M Bula, J Davila-Vel, LA Akay, L Zhu, A Frank, MB Victor, JM Bonner, H Mathys, YT Lin, T Ko, DA Bennett, HP Cam, M Kellis, LH Tsai
    Nat. Med., 2020;26(6):952-963.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Initiation of reticular and spider veins, incompetent perforantes and varicose veins in the saphenous vein network of the rat
    Authors: BB Patai, G Dornyei, AM Tokes, JR Hetthessy, A Fees, GL Nadasy
    Scientific Reports, 2020;10(1):15381.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  26. STRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells
    Authors: L Jin, Y Chen, DK Crossman, A Datta, T Vu, JA Mobley, MK Basu, M Scarduzio, H Wang, C Chang, PK Datta
    Nat Commun, 2020;11(1):5941.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. Cellular census of human fibrosis defines functionally distinct stromal cell types and states
    Authors: TB Layton, L Williams, F McCann, M Zhang, M Fritzsche, H Colin-York, M Cabrita, MTH Ng, M Feldmann, SN Sansom, D Furniss, W Xie, J Nanchahal
    Nat Commun, 2020;11(1):2768.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  28. Dynamic Changes in Function and Proteomic Composition of Extracellular Vesicles from Hepatic Stellate Cells during Cellular Activation
    Authors: X Li, R Chen, S Kemper, DR Brigstock
    Cells, 2020;9(2):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  29. Adipose Mesenchymal Cells-Derived EVs Alleviate DOCA-Salt-Induced Hypertension by Promoting Cardio-Renal Protection
    Authors: RS Lindoso, JA Lopes, R Binato, E Abdelhay, CM Takiya, KR Miranda, LS Lara, A Viola, B Bussolati, A Vieyra, F Collino
    Mol Ther Methods Clin Dev, 2020;16(0):63-77.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  30. Pathological Study on Epithelial-Mesenchymal Transition in Silicotic Lung Lesions in Rat
    Authors: M Komai, K Mihira, A Shimada, I Miyamoto, K Ogihara, Y Naya, T Morita, K Inoue, H Takano
    Vet Sci, 2019;6(3):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  31. Human induced pluripotent stem cell line HMUi001-A derived from corneal stromal cells
    Authors: T Bikkuzin, Y Shi, B Sun, Y Guo, X Jin, Z Han, V Pavlov, H Zhang
    Stem Cell Res, 2019;37(0):101409.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  32. Inhibition of PAI-1 limits chemotherapy resistance in lung cancer through suppressing myofibroblast characteristics of cancer-associated fibroblasts
    Authors: T Masuda, T Nakashima, M Namba, K Yamaguchi, S Sakamoto, Y Horimasu, S Miyamoto, H Iwamoto, K Fujitaka, Y Miyata, H Hamada, M Okada, N Hattori
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  33. Fibroblast-like synovial cell production of extra domain A fibronectin associates with inflammation in osteoarthritis
    Authors: TW Kragstrup, DH Sohn, CM Lepus, K Onuma, Q Wang, WH Robinson, J Sokolove
    BMC Rheumatol, 2019;3(0):46.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  34. Empagliflozin reduces high glucose-induced oxidative stress and miR-21-dependent TRAF3IP2 induction and RECK suppression, and inhibits human renal proximal tubular epithelial cell migration and epithelial-to-mesenchymal transition
    Authors: NA Das, AJ Carpenter, A Belenchia, AR Aroor, M Noda, U Siebenlist, B Chandrasek, VG DeMarco
    Cell. Signal., 2019;68(0):109506.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. Optimal stimulation toward the dermal papilla lineage can be promoted by combined use of osteogenic and adipogenic inducers
    Authors: T Kazi, I Niibe, A Nishikawa, T Matsuzaki
    FEBS Open Bio, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  36. Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds
    Authors: CF Guerrero-J, PH Dedhia, S Jin, R Ruiz-Vega, D Ma, Y Liu, K Yamaga, O Shestova, DL Gay, Z Yang, K Kessenbroc, Q Nie, WS Pear, G Cotsarelis, MV Plikus
    Nat Commun, 2019;10(1):650.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  37. TGF-?-SNAIL axis induces M�ller glial-mesenchymal transition in the pathogenesis of idiopathic epiretinal membrane
    Authors: A Kanda, K Noda, I Hirose, S Ishida
    Sci Rep, 2019;9(1):673.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells
    Authors: J Barnes, F Salas, R Mokhtari, H Dolstra, E Pedrosa, HM Lachman
    Mol Autism, 2018;9(0):44.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  39. Small non-coding RNA landscape of extracellular vesicles from human stem cells
    Authors: S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ
    Sci Rep, 2018;8(1):15503.
  40. Implication of molecular vascular smooth muscle cell heterogeneity among arterial beds in arterial calcification
    Authors: O Espitia, M Chatelais, M Steenman, C Charrier, B Maurel, S Georges, R Houlgatte, F Verrecchia, B Ory, F Lamoureux, D Heymann, Y Gouëffic, T Quillard
    PLoS ONE, 2018;13(1):e0191976.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  41. Ebola Virus Causes Intestinal Tract Architectural Disruption and Bacterial Invasion in Non-Human Primates
    Authors: RB Reisler, X Zeng, CW Schellhase, JJ Bearss, TK Warren, JC Trefry, GW Christophe, MG Kortepeter, S Bavari, AP Cardile
    Viruses, 2018;10(10):.
    Species: Primate - Macaca mulatta (Rhesus Macaques)
    Sample Types: Whole Tissue
    Applications: IHC-P
  42. Establishment and characterization of five immortalized human scalp dermal papilla cell lines
    Authors: MH Kwack, JM Yang, GH Won, MK Kim, JC Kim, YK Sung
    Biochem. Biophys. Res. Commun., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  43. Wnt inhibition promotes vascular specification of embryonic cardiac progenitors
    Authors: DE Reichman, L Park, L Man, D Redmond, K Chao, RP Harvey, MM Taketo, Z Rosenwaks, D James
    Development, 2018;145(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  44. Exosomal miR-21-5p derived from gastric cancer promotes peritoneal metastasis via mesothelial-to-mesenchymal transition
    Authors: Q Li, B Li, Q Li, S Wei, Z He, X Huang, L Wang, Y Xia, Z Xu, Z Li, W Wang, L Yang, D Zhang, Z Xu
    Cell Death Dis, 2018;9(9):854.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  45. Cancer-associated fibroblasts promote an immunosuppressive microenvironment through the induction and accumulation of protumoral macrophages
    Authors: H Takahashi, K Sakakura, T Kudo, M Toyoda, K Kaira, T Oyama, K Chikamatsu
    Oncotarget, 2017;8(5):8633-8647.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  46. Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-?B and MURF1 levels
    Authors: L Zhou, Y Huang, H Xie, X Mei
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  47. P2X7 receptor antagonism modulates IL-1? and MMP9 in human atherosclerotic vessels
    Authors: M Lombardi, ME Mantione, D Baccellier, D Ferrara, R Castellano, R Chiesa, O Alfieri, C Foglieni
    Sci Rep, 2017;7(1):4872.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  48. Generation of a human induced pluripotent stem cell line CERAi001-A-6 using episomal vectors
    Authors: RCB Wong, SS Hung, S Jackson, V Singh, S Khan, HH Liang, LS Kearns, T Nguyen, A Conquest, M Daniszewsk, AW Hewitt, A Pébay
    Stem Cell Res, 2017;22(0):13-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  49. KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis
    Authors: H Kamata, Y Tsukasaki, T Sakai, R Ikebe, J Wang, A Jeffers, J Boren, S Owens, T Suzuki, M Higashihar, S Idell, TA Tucker, M Ikebe
    Sci Rep, 2017;7(1):4556.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  50. NOTCH1-dependent nitric oxide signaling deficiency in hypoplastic left heart syndrome revealed through patient-specific phenotypes detected in bioengineered cardiogenesis
    Authors: SC Hrstka, X Li, TJ Nelson,
    Stem Cells, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  51. Mitochondrial replacement in an iPSC model of Leber's hereditary optic neuropathy
    Authors: RCB Wong, SY Lim, SSC Hung, S Jackson, S Khan, NJ Van Bergen, E De Smit, HH Liang, LS Kearns, L Clarke, DA Mackey, AW Hewitt, IA Trounce, A Pébay
    Aging (Albany NY), 2017;9(4):1341-1350.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  52. Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane
    Authors: Y Dong, A Kanda, K Noda, W Saito, S Ishida
    Sci Rep, 2017;7(0):44266.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  53. Neuropathogenesis of Zika Virus in a Highly Susceptible Immunocompetent Mouse Model after Antibody Blockade of Type I Interferon
    Authors: DR Smith, B Hollidge, S Daye, X Zeng, C Blancett, K Kuszpit, T Bocan, JW Koehler, S Coyne, T Minogue, T Kenny, X Chi, S Yim, L Miller, C Schmaljohn, S Bavari, JW Golden
    PLoS Negl Trop Dis, 2017;11(1):e0005296.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  54. Generation of Induced Cardiospheres via Reprogramming of Skin Fibroblasts for Myocardial Regeneration
    Authors: Jian-Yong Xu
    Stem Cells, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  55. Exosomes in Human Breast Milk Promote EMT
    Authors: W Qin, Y Tsukasaki, S Dasgupta, N Mukhopadhy, M Ikebe, ER Sauter
    Clin Cancer Res, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  56. Cancer-associated mesenchymal stroma fosters the stemness of osteosarcoma cells in response to intratumoral acidosis via NF-?B acivation
    Authors: Sofia Avnet
    Int. J. Cancer, 2016;0(0):.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC
  57. Human pancreatic cancer cells induce a MyD88-dependent stromal response to promote a tumor-tolerant immune microenvironment
    Cancer Res., 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  58. The influence of warm ischemia elimination on kidney injury during transplantation - clinical and molecular study
    Sci Rep, 2016;6(0):36118.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  59. CD105 expression on CD34-negative spindle-shaped stromal cells of primary tumor is an unfavorable prognostic marker in early breast cancer patients.
    Authors: Martinez L, Labovsky V, Calcagno M, Davies K, Garcia Rivello H, Bianchi M, Wernicke A, Fernandez Vallone V, Chasseing N
    PLoS ONE, 2015;10(3):e0121421.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  60. Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
    Authors: Riordan D, Varma S, West R, Brown P
    PLoS ONE, 2015;10(7):e0128975.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  61. Association between PON1 rs662 polymorphism and coronary artery disease.
    Authors: Liu T, Zhang X, Zhang J, Liang Z, Cai W, Huang M, Yan C, Zhu Z, Han Y
    Eur J Clin Nutr, 2014;68(9):1029-35.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  62. Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability.
    Authors: Davaine J, Quillard T, Brion R, Laperine O, Guyomarch B, Merlini T, Chatelais M, Guilbaud F, Brennan M, Charrier C, Heymann D, Goueffic Y, Heymann M
    PLoS ONE, 2014;9(9):e107642.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  63. Efficient generation of integration-free human induced pluripotent stem cells from keratinocytes by simple transfection of episomal vectors.
    Authors: Piao Y, Hung S, Lim S, Wong R, Ko M
    Stem Cells Transl Med, 2014;3(0):787-791.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  64. Occurrence of tertiary lymphoid tissue is associated with T-cell infiltration and predicts better prognosis in early-stage colorectal cancers.
    Authors: Di Caro G, Bergomas F, Grizzi F, Doni A, Bianchi P, Malesci A, Laghi L, Allavena P, Mantovani A, Marchesi F
    Clin Cancer Res, 2014;20(8):2147-58.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  65. Distinct transcriptional profiles of angioblasts derived from human embryonic stem cells.
    Authors: Song S, Jung W, Kim K, Hong W, Kim H, Lee K, Lee K, Suh W
    Exp Cell Res, 2013;319(8):1136-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  66. Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype.
    Authors: van der Veer E, de Bruin R, Kraaijeveld A, de Vries M, Bot I, Pera T, Segers F, Trompet S, van Gils J, Roeten M, Beckers C, van Santbrink P, Janssen A, van Solingen C, Swildens J, de Boer H, Peters E, Bijkerk R, Rousch M, Doop M, Kuiper J, Schalij M, van der Wal A, Richard S, van Berkel T, Pickering J, Hiemstra P, Goumans M, Rabelink T, de Vries A, Quax P, Jukema J, Biessen E, van Zonneveld A
    Circ Res, 2013;113(9):1065-75.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  67. Transcriptional effects of 1,25 dihydroxyvitamin D(3) physiological and supra-physiological concentrations in breast cancer organotypic culture.
    Authors: Milani C, Katayama M, de Lyra E, Welsh J, Campos L, Brentani M, Maciel M, Roela R, del Valle P, Goes J, Nonogaki S, Tamura R, Folgueira M
    BMC Cancer, 2013;13(0):119.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  68. Locally different endothelial nitric oxide synthase protein levels in ascending aortic aneurysms of bicuspid and tricuspid aortic valve.
    Authors: Mohamed SA, Radtke A, Saraei R
    Cardiol Res Pract, 2012;2012(0):165957.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  69. Inhibition of caspase-3 differentially affects vascular smooth muscle cell apoptosis in the concave versus convex aortic sites in ascending aneurysms with a bicuspid aortic valve.
    Authors: Mohamed SA, Misfeld M, Hanke T, Charitos EI, Bullerdiek J, Belge G, Kuehnel W, Sievers HH
    Ann. Anat., 2010;192(3):145-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  70. The effect of VEGF on the myogenic differentiation of adipose tissue derived stem cells within thermosensitive hydrogel matrices.
    Authors: Kim MH, Hong HN, Hong JP, Park CJ, Kwon SW, Kim SH, Kang G, Kim M
    Biomaterials, 2010;31(6):1213-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  71. Lysosome-dependent Ca(2+) release response to Fas activation in coronary arterial myocytes through NAADP: evidence from CD38 gene knockouts.
    Authors: Zhang F, Xia M, Li PL
    Am. J. Physiol., Cell Physiol., 2010;298(5):C1209-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  72. Effect of an antimicrobial agent on atherosclerotic plaques: assessment of metalloproteinase activity by molecular imaging.
    Authors: Ohshima S, Fujimoto S, Petrov A, Nakagami H, Haider N, Zhou J, Tahara N, Osako MK, Fujimoto A, Zhu J, Murohara T, Edwards DS, Narula N, Wong ND, Chandrashekhar Y, Morishita R, Narula J
    J. Am. Coll. Cardiol., 2010;55(12):1240-9.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-P
  73. Hypoxia-induced alveolar epithelial-mesenchymal transition requires mitochondrial ROS and hypoxia-inducible factor 1.
    Authors: Zhou G, Dada LA, Wu M, Kelly A, Trejo H, Zhou Q, Varga J, Sznajder JI
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(6):L1120-30.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  74. Gadodiamide contrast agent 'activates' fibroblasts: a possible cause of nephrogenic systemic fibrosis.
    Authors: Edward M, Quinn JA, Mukherjee S, Jensen MB, Jardine AG, Mark PB, Burden AD
    J. Pathol., 2008;214(5):584-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  75. Role of matrix metalloprotease-9 in hyperoxic injury in developing lung.
    Authors: Chetty A, Cao GJ, Severgnini M, Simon A, Warburton R, Nielsen HC
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008;295(4):L584-92.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  76. Breast carcinoma-associated fibroblasts and their counterparts display neoplastic-specific changes.
    Authors: Hawsawi NM, Ghebeh H, Hendrayani SF, Tulbah A, Al-Eid M, Al-Tweigeri T, Ajarim D, Alaiya A, Dermime S, Aboussekhra A
    Cancer Res., 2008;68(8):2717-25.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  77. Molecular imaging of matrix metalloproteinase in atherosclerotic lesions: resolution with dietary modification and statin therapy.
    Authors: Fujimoto S, Hartung D, Ohshima S, Edwards DS, Zhou J, Yalamanchili P, Azure M, Fujimoto A, Isobe S, Matsumoto Y, Boersma H, Wong N, Yamazaki J, Narula N, Petrov A, Narula J
    J. Am. Coll. Cardiol., 2008;52(23):1847-57.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  78. Bronchoalveolar lavage fluid from patients with acute lung injury/acute respiratory distress syndrome induces myofibroblast differentiation.
    Authors: Synenki L, Chandel NS, Budinger GR, Donnelly HK, Topin J, Eisenbart J, Jovanovic B, Jain M
    Crit. Care Med., 2007;35(3):842-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  79. Differentiation of human circulating fibrocytes as mediated by transforming growth factor-beta and peroxisome proliferator-activated receptor gamma.
    Authors: Hong KM, Belperio JA, Keane MP, Burdick MD, Strieter RM
    J. Biol. Chem., 2007;282(31):22910-20.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  80. Establishment and characterization of an immortalized human hepatic stellate cell line for applications in co-culturing with immortalized human hepatocytes.
    Authors: Pan X, Wang Y, Yu X, Li J, Zhou N, Du W, Zhang Y, Cao H, Zhu D, Chen Y, Li L
    Int J Med Sci, 0;12(3):248-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Average Rating: 4.9 (Based on 8 Reviews)

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Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Anonymous on 10/19/2021
Application: WB Sample Tested: Endothelial cells Species: Mouse

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Anonymous on 08/10/2021
Application: IHC Sample Tested: Testicular tissue Species: Mouse

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Anonymous on 08/04/2021
Application: IHC Sample Tested: Muscle tissue Species: Mouse

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Anonymous on 02/19/2019
Application: IHC Sample Tested: Breast cancer tissue Species: Human

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Anonymous on 07/21/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Anonymous on 12/28/2017
Application: ICC/IF Sample Tested: HMVEC human microvascular endothelial cells Species: Human

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Li Liu on 10/20/2017
Application: WB Sample Tested: Skin tissue Species: Mouse

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Xiaoming Zhou on 10/20/2017
Application: WB Sample Tested: Lung tissue Species: Mouse

The antibody works well for lung tissue of mouse.