Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Catalog # Availability Size / Price Qty
MAB1420
MAB1420-SP
Best Seller
Detection of Human  alpha ‑Smooth Muscle Actin by Western Blot.
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Product Details
Citations (127)
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Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human  alpha -Smooth Muscle Actin.
Source
Monoclonal Mouse IgG2A Clone # 1A4
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Peptide corresponding to residues EEEDSTALVC of the N-term of human alpha -smooth muscle actin (1)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1-0.25 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Dual RNAscope ISH-IHC
0.5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human a-Smooth Muscle Actin antibody by Western Blot. View Larger

Detection of Human alpha ‑Smooth Muscle Actin by Western Blot. Western blot shows lysates of human heart tissue and iBJ6 human induced pluripotent stem cell line undifferentiated or cardiac differentiated. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/ Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Smooth Muscle Actin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse and Rat a-Smooth Muscle Actin antibody by Western Blot. View Larger

Detection of Mouse and Rat alpha ‑Smooth Muscle Actin by Western Blot. Western blot shows lysates of mouse colon tissue, C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, and rat colon tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Smooth Muscle Actin at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry a-Smooth Muscle Actin antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

alpha ‑Smooth Muscle Actin in BG01V Human Embryonic Stem Cells. a-Smooth Muscle Actin was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiomyocytes using Mouse Anti-Human/Mouse/ Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry a-Smooth Muscle Actin antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

alpha ‑Smooth Muscle Actin in Human Heart. a-Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry a-Smooth Muscle Actin antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

alpha -Smooth Muscle Actin in Human Breast Cancer Tissue. a-Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/ Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human a-Smooth Muscle Actin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human alpha ‑Smooth Muscle Actin by Simple WesternTM. Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyocytes (+), loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Smooth Muscle Actin at approximately 49 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Intracellular Staining by Flow Cytometry Detection of a-Smooth Muscle Actin antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human/Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Dual RNAscope ISH-IHC Compatible alpha-Smooth Muscle Actin antibody in Human Breast Cancer Tissue Using Dual RNAscope<sup>®</sup>ISH and IHC. View Larger

alpha-Smooth Muscle Actin in Human Breast Cancer Tissue Using Dual RNAscope®ISH and IHC. alpha-Smooth Muscle Actin mRNA was detected (top image) in formalin-fixed paraffin-embedded tissue sections of human breast cancer tissue probed with ACD RNAScope® Probe (Catalog # 444771-C2) and stained using ACD RNAscope® 2.5 HD Duplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section (bottom image) was processed for immunohistochemistry using R&D Systems Mouse Anti-Human/Mouse/Rat alpha-Smooth Muscle Actin Monoclonal Antibody (Catalog# MAB1420) at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001) and DAB chromogen (brown). Tissues were counterstained with hematoxylin (blue).

Immunocytochemistry/ Immunofluorescence Detection of Human alpha-Smooth Muscle Actin by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human alpha-Smooth Muscle Actin by Immunocytochemistry/Immunofluorescence Overview of Multi-dimensional Microscopic Molecular Profiling (MMMP).The overall MMMP approach is depicted using an example tissue section from normal human duodenum (sample #1.9.7). (a) Slides were subjected to repeated cycles of staining and imaging with fluorescent primary antibodies and DAPI. At the end of each cycle, fluorescent signal was removed by a chemical bleaching process, and slides were again imaged, before proceeding to the next round of this iterative procedure. After the final antibody stain (#15 Sma), slides were analyzed with a series of histochemical stains. (b) A set of tiling images spanning each tissue section was initially generated by the microscope system. The tiling images were then computationally ‘stitched’ together to produce a single image per staining cycle for each sample. (c) Image registration was performed to align images from the same tissue section across cycles. Mean intensities of the DAPI signal from all immuno-fluorescence images are shown from before (Unregistered) and after (Registered) the image registration procedure was completed. (d) Following registration, signal intensities from the relevant channels for each image (columns) in the MMMP series were extracted for each pixel (rows) within the tissue section and compiled into a large data matrix of in situ molecular profiles. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0128975), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human alpha-Smooth Muscle Actin by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human alpha-Smooth Muscle Actin by Immunocytochemistry/Immunofluorescence Functional glycosylation of alpha ‐dystroglycan and characterization of dystroglycanopathy patient‐specific iPSCsCurrent model of the core M3 functional glycan structure on alpha ‐dystroglycan and enzymes involved in its synthesis. ECM ligands, such as laminins, bind to the Xyl‐GlucA disaccharide repeats (IIH6 epitope). Man, mannose; GlcNAc, N‐acetylglucosamine; GalNAc, N‐acetylgalactosamine; Rbo5P, ribitol‐5‐phosphate; Xyl, xylose; GlcA, glucuronic acid.Representative images of immunostaining demonstrate that FKRP‐iPSCs express specific pluripotency‐associated markers, including NANOG, OCT4, Tra‐1‐60, and SSEA4.FKRP‐iPSCs have a normal karyotype.In vitro differentiation of FKRP‐iPSCs to cells representing ectoderm ( beta ‐III Tubulin, Tuj1), mesoderm (SMA, smooth muscle actin), and endoderm (AFP, alpha ‐fetoprotein).Data information: Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31566294), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Smooth Muscle Actin

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

References
  1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
  2. Oishi, K. et al. (2002) J. Physiol. 540:139.
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Entrez Gene IDs
59 (Human); 11475 (Mouse); 81633 (Rat)
Alternate Names
AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin

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Citations for Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

127 Citations: Showing 1 - 10
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  1. Astragaloside IV regulates NF-kappa B mediated cellular senescence and apoptosis of hepatic stellate cells to suppress PDGF BB induced activation
    Authors: Chen Z, Yao L, Liu Y et al.
    Exp Ther Med
  2. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease.
    Authors: Kinchen J, Chen HH, Parikh K et al.
    Cell
  3. Engineered Fibroblast Extracellular Vesicles Attenuate Pulmonary Inflammation and Fibrosis in Bleomycin-Induced Lung Injury
    Authors: Abdulrahman Ibrahim, Alessandra Ciullo, Chang Li, Akbarshakh Akhmerov, Kiel Peck, K. C. Jones-Ungerleider et al.
    Frontiers in Cell and Developmental Biology
  4. Fetal and trophoblast PI3K p110 alpha have distinct roles in regulating resource supply to the growing fetus in mice
    Authors: Jorge López-Tello, Vicente Pérez-García, Jaspreet Khaira, Laura C Kusinski, Wendy N Cooper, Adam Andreani et al.
    eLife
  5. Downregulation of syndecan?1 expression induces activation of hepatic stellate cells via the TGF??1/Smad3 signaling pathway
    Authors: M Deng, L Xu, X Xie, X Sheng, Z Zou, M Yao
    Mol Med Rep, 2019-05-09;0(0):.
  6. Generation of a gene-corrected human isogenic iPSC line from an Alzheimer's disease iPSC line carrying the London mutation in APP (V717I)
    Authors: D Hernández, S Morgan Sch, M Daniszewsk, CM Karch, Dominantly, AM Goate, A Pébay
    Stem Cell Research, 2021-04-30;53(0):102373.
  7. Evolution of nasal and olfactory infection characteristics of SARS-CoV-2 variants
    Authors: Chen M, Pekosz A, Villano JS et al.
    bioRxiv : the preprint server for biology
  8. Eupatilin Ameliorates Hepatic Fibrosis and Hepatic Stellate Cell Activation by Suppressing beta-catenin/PAI-1 Pathway
    Authors: J Hu, Y Liu, Z Pan, X Huang, J Wang, W Cao, Z Chen
    International Journal of Molecular Sciences, 2023-03-21;24(6):.
  9. Comparison of Tumor- and Bone Marrow-Derived Mesenchymal Stromal/Stem Cells from Patients with High-Grade Osteosarcoma
    Authors: LR Le Nail, M Brennan, P Rosset, F Deschaseau, P Piloquet, O Pichon, C Le Caignec, V Crenn, P Layrolle, O Hérault, G De Pinieux, V Trichet
    Int J Mol Sci, 2018-03-01;19(3):.
  10. Generation and characterization of two fibroblast-derived Baboon induced pluripotent stem cell lines
    Authors: Jocher, J;Edenhofer, FC;Müller, S;Janssen, P;Briem, E;Geuder, J;Enard, W;
    Stem cell research
    Species: Primate - Papio anubis (Olive Baboon)
    Sample Types: Embryoid Bodies
    Applications: Immunocytochemistry
  11. Iguratimod attenuated fibrosis in systemic sclerosis via targeting early growth response 1 expression
    Authors: Shen, L;Yin, H;Sun, L;Zhang, Z;Jin, Y;Cao, S;Fu, Q;Fan, C;Bao, C;Lu, L;Zhan, Y;Xu, X;Chen, X;Yan, Q;
    Arthritis research & therapy
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. An integrated organoid omics map extends modeling potential of kidney disease
    Authors: Lassé, M;El Saghir, J;Berthier, CC;Eddy, S;Fischer, M;Laufer, SD;Kylies, D;Hutzfeldt, A;Bonin, LL;Dumoulin, B;Menon, R;Vega-Warner, V;Eichinger, F;Alakwaa, F;Fermin, D;Billing, AM;Minakawa, A;McCown, PJ;Rose, MP;Godfrey, B;Meister, E;Wiech, T;Noriega, M;Chrysopoulou, M;Brandts, P;Ju, W;Reinhard, L;Hoxha, E;Grahammer, F;Lindenmeyer, MT;Huber, TB;Schlüter, H;Thiel, S;Mariani, LH;Puelles, VG;Braun, F;Kretzler, M;Demir, F;Harder, JL;Rinschen, MM;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC/IF
  13. Single-Cell RNA Sequencing Unifies Developmental Programs of Esophageal and Gastric Intestinal Metaplasia
    Authors: Karol Nowicki-Osuch, Lizhe Zhuang, Tik Shing Cheung, Emily L. Black, Neus Masqué-Soler, Ginny Devonshire et al.
    Cancer Discovery
  14. Combinatorial Microgels for 3D ECM Screening and Heterogeneous Microenvironmental Culture of Primary Human Hepatic Stellate Cells
    Authors: Ryoo, H;Underhill, GH;
    bioRxiv : the preprint server for biology
  15. An unbiased proteomics approach to identify the senescence-associated secretory phenotype of human bone marrow-derived mesenchymal stem cells
    Authors: RM Samsonraj, SF Law, A Chandra, RJ Pignolo
    Bone Reports, 2023-03-21;18(0):101674.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  16. Co-culture models of endothelial cells, macrophages, and vascular smooth muscle cells for the study of the natural history of atherosclerosis
    Authors: M Liu, S Samant, CH Vasa, RM Pedrigi, UM Oguz, S Ryu, T Wei, DR Anderson, DK Agrawal, YS Chatzizisi
    PLoS ONE, 2023-01-20;18(1):e0280385.
    Species: Human
    Sample Types: Protein, Whole Cells
    Applications: ICC, Western Blot
  17. Targeting Periostin Expression Makes Pancreatic Cancer Spheroids More Vulnerable to Natural Killer Cells
    Authors: D Karakas, M Erkisa, RO Akar, G Akman, EY Senol, E Ulukaya
    Biomedicines, 2023-01-19;11(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Dietary oxidized frying oil activates hepatic stellate cells and accelerates the severity of carbon tetrachloride- and thioacetamide-induced liver fibrosis in mice
    Authors: FM Suk, FY Hsu, YC Lee, TL Chen, WC Chiu, YJ Liao
    The Journal of nutritional biochemistry, 2023-01-11;0(0):109267.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: IHC, Western Blot
  19. Human theca arises from ovarian stroma and is comprised of three discrete subtypes
    Authors: Nicole Lustgarten Guahmich, Limor Man, Jerry Wang, Laury Arazi, Eleni Kallinos, Ariana Topper-Kroog et al.
    Communications Biology
  20. Regeneration difficulties in patients with FQAD can limit the use of iPSc-based cell therapy
    Authors: Dagmara Grot, Katarzyna Wasiak, Jerzy Tyszkowski, Ewelina Stoczynska-Fidelus, Tomasz P. Ochedalski, Piotr Rieske
    Stem Cell Research & Therapy
  21. CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype
    Authors: W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang
    Oncogene, 2022-11-22;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment
    Authors: D Zheng, J Zhou, L Qian, X Liu, C Chang, S Tang, H Zhang, S Zhou
    Bioactive materials, 2022-11-03;22(0):567-587.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Inhibition of ROCK ameliorates pulmonary fibrosis by suppressing M2 macrophage polarisation through phosphorylation of STAT3
    Authors: Qingfang Li, Yuan Cheng, Zhe Zhang, Zhenfei Bi, Xuelei Ma, Yuquan Wei et al.
    Clinical and Translational Medicine
  24. Alcohol and HIV-Derived Hepatocyte Apoptotic Bodies Induce Hepatic Stellate Cell Activation
    Authors: M New-Aaron, RS Dagur, SS Koganti, M Ganesan, W Wang, E Makarov, M Ogunnaike, KK Kharbanda, LY Poluektova, NA Osna
    Biology, 2022-07-14;11(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient
    Authors: J Lotila, T Hyvärinen, H Skottman, L Airas, S Narkilahti, S Hagman
    Oncogene, 2022-07-11;63(0):102865.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  26. AAV2-mediated gene therapy for Bietti crystalline dystrophy provides functional CYP4V2 in multiple relevant cell models
    Authors: JH Wang, GE Lidgerwood, M Daniszewsk, ML Hu, GE Roberts, RCB Wong, SSC Hung, ME McClements, AW Hewitt, A Pébay, DG Hickey, TL Edwards
    Oncogene, 2022-06-09;12(1):9525.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  27. Histochemical examination of blood vessels in murine femora with intermittent PTH administration
    Authors: H Maruoka, S Zhao, H Yoshino, M Abe, T Yamamoto, H Hongo, M Haraguchi-, A Nasoori, H Ishizu, Y Nakajima, M Omaki, T Shimizu, N Iwasaki, PH Luiz de Fr, M Li, T Hasegawa
    Journal of oral biosciences, 2022-05-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. Myo5b Transports Fibronectin-Containing Vesicles and Facilitates FN1 Secretion from Human Pleural Mesothelial Cells
    Authors: T Sakai, YY Choo, O Sato, R Ikebe, A Jeffers, S Idell, T Tucker, M Ikebe
    International Journal of Molecular Sciences, 2022-04-27;23(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  29. Generation of a human induced pluripotent stem cell line derived from a patient with dilated cardiomyopathy carrying LMNA nonsense mutation
    Authors: Y Shimoda, N Murakoshi, H Mori, D Xu, K Tajiri, Y Hemmi, I Sato, M Noguchi, Y Nakamura, Y Hayashi, M Ieda
    Stem Cell Research, 2022-04-27;62(0):102793.
    Species: Human
    Sample Types: Spheroid
    Applications: ICC
  30. Signature of the vascular tumor microenvironment as a marker of the therapeutic response to doxorubicin in a preclinical model of osteosarcoma
    Authors: V Crenn, J Amiaud, A Gomez-Brou, V Potiron, F Gouin, P Rosset, LL Nail, L Vidal, H Bertin, R Brion, G Tran, F Verrecchia, I Corre, F Redini
    American journal of cancer research, 2022-04-15;12(4):1843-1854.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  31. A vasculature niche orchestrates stromal cell phenotype through PDGF signaling: Importance in human fibrotic disease
    Authors: TB Layton, L Williams, N Yang, M Zhang, C Lee, M Feldmann, G Trujillo, D Furniss, J Nanchahal
    Proceedings of the National Academy of Sciences of the United States of America, 2022-03-23;119(13):e2120336119.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: CyTof, IHC
  32. CRISPR/Cas9-engineered human ES cells harboring heterozygous and homozygous c-KIT knockout
    Authors: MAS de Toledo, X Fu, F Kluge, K Götz, S Schmitz, P Wanek, HM Schüler, K Pannen, N Chatain, S Koschmiede, TH Brümmendor, M Zenke
    Stem Cell Research, 2022-03-01;60(0):102732.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  33. Switching in the expression pattern of actin isoforms marks the onset of contractility and distinct mechanodynamic behavior during cardiomyocyte differentiation
    Authors: RH Pires, TH Dau, E Manu, N Shree, O Otto
    Physiological Reports, 2022-02-01;10(3):e15171.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  34. Systemic deficiency of vitronectin is associated with aortic inflammation and plaque progression in ApoE‐Knockout mice
    Authors: Devasmita Chakravarty, Aleepta Guha Ray, Vivek Chander, Ulaganathan Mabalirajan, Prakash Chandra Mondal, Khawer N. Siddiqui et al.
    FASEB BioAdvances
  35. Mirodenafil ameliorates skin fibrosis in bleomycin-induced mouse model of systemic sclerosis
    Authors: Jong Seong Roh, Hoim Jeong, Beomgu Lee, Byung Wook Song, Seung Jin Han, Dong Hyun Sohn et al.
    Animal Cells and Systems
  36. Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis
    Authors: SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn
    International Journal of Molecular Sciences, 2022-01-14;23(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  37. Dermal Papilla Cell-Derived Extracellular Vesicles Increase Hair Inductive Gene Expression in Adipose Stem Cells via beta-Catenin Activation
    Authors: T Kazi, A Nagata, T Nakagawa, T Matsuzaki, S Inui
    Cells, 2022-01-07;11(2):.
    Species: Mouse
    Sample Types: Spheroids
    Applications: ICC
  38. Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction)
    Authors: K Hira, P Sharma, A Mahale, O Prakash Ku, A Sajeli Beg
    International immunopharmacology, 2021-12-29;103(0):108494.
    Species: Mouse, Rat
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  39. Pharmacological targeting of Sam68 functions in colorectal cancer stem cells
    Authors: Angelique N. Masibag, Christopher J. Bergin, Joshua R. Haebe, Aïcha Zouggar, Muhammad S. Shah, Tamara Sandouka et al.
    iScience
  40. Adult spiny mice (Acomys) exhibit endogenous cardiac recovery in response to myocardial infarction
    Authors: Hsuan Peng, Kazuhiro Shindo, Renée R. Donahue, Erhe Gao, Brooke M. Ahern, Bryana M. Levitan et al.
    npj Regenerative Medicine
  41. Generation of an induced pluripotent stem cell line GZHMCi008-A derived from a patient with SRY-positive 46,XX testicular disorder of sex development
    Authors: Y Luo, Y Chen, M Zhang, X Ma, D Zhu, Y Chen
    Stem Cell Research, 2021-10-22;57(0):102583.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  42. Synthetic VSMCs induce BBB disruption mediated by MYPT1 in ischemic stroke
    Authors: Hailan Meng, Lizhen Fan, Cun-Jin Zhang, Liwen Zhu, Pinyi Liu, Jian Chen et al.
    iScience
  43. Synergistic effects of silver nanoparticles and cisplatin in combating inflammation and hyperplasia of airway stents
    Authors: Z Li, C Tian, D Jiao, J Li, Y Li, X Zhou, H Zhao, Y Zhao, X Han
    Bioactive materials, 2021-07-29;9(0):266-280.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  44. Normalization of Enzyme Expression and Activity Regulating Vitamin A Metabolism Increases RAR-Beta Expression and Reduces Cellular Migration and Proliferation in Diseases Caused by Tuberous Sclerosis Gene Mutations
    Authors: Elhusseiny Mohamed Mahmoud Abdelwahab, Judit Bovari-Biri, Gabor Smuk, Tunde Harko, Janos Fillinger, Judit Moldvay et al.
    Frontiers in Oncology
  45. SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury
    Authors: Anteneh Getachew, Nasir Abbas, Kai You, Zhen Yang, Muzammal Hussain, Xinping Huang et al.
    iScience
  46. Generation of three induced pluripotent stem cell lines, SCVIi003-A, SCVIi004-A, SCVIi005-A, from patients with ARVD/C caused by heterozygous mutations in the PKP2 gene
    Authors: JWS Jahng, KE Black, L Liu, HR Bae, M Perez, EA Ashley, K Sallam, JC Wu
    Stem Cell Research, 2021-03-12;53(0):102284.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  47. Cancer-associated fibroblast-derived SDF-1 induces epithelial-mesenchymal transition of lung adenocarcinoma via CXCR4/&beta-catenin/PPAR&delta signalling
    Authors: Y Wang, W Lan, M Xu, J Song, J Mao, C Li, X Du, Y Jiang, E Li, R Zhang, Q Wang
    Cell Death & Disease, 2021-02-26;12(2):214.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  48. A non-invasive method to generate induced pluripotent stem cells from primate urine
    Authors: J Geuder, LE Wange, A Janjic, J Radmer, P Janssen, JW Bagnoli, S Müller, A Kaul, M Ohnuki, W Enard
    Scientific Reports, 2021-02-10;11(1):3516.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  49. Cohort Analysis of ADAM8 Expression in the PDAC Tumor Stroma
    Authors: C Jaworek, Y Verel-Yilm, S Driesch, S Ostgathe, L Cook, S Wagner, DK Bartsch, EP Slater, JW Bartsch
    Journal of personalized medicine, 2021-02-10;11(2):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  50. Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9
    Authors: MR Vitale, JEM Zöller, C Jansch, A Janz, F Edenhofer, E Klopocki, D van den Ho, T Vanmierlo, O Rivero, N Nadif Kasr, GC Ziegler, KP Lesch
    Stem Cell Research, 2021-01-11;51(0):102169.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  51. STRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells
    Authors: L Jin, Y Chen, DK Crossman, A Datta, T Vu, JA Mobley, MK Basu, M Scarduzio, H Wang, C Chang, PK Datta
    Nat Commun, 2020-11-23;11(1):5941.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  52. Thrombospondin‑2 is upregulated in patients with aortic dissection and enhances angiotensin II‑induced smooth muscle cell apoptosis
    Authors: Liping Qi, Kui Wu, Shutian Shi, Qingwei Ji, Huangtai Miao, Que Bin
    Experimental and Therapeutic Medicine
  53. Initiation of reticular and spider veins, incompetent perforantes and varicose veins in the saphenous vein network of the rat
    Authors: BB Patai, G Dornyei, AM Tokes, JR Hetthessy, A Fees, GL Nadasy
    Scientific Reports, 2020-09-21;10(1):15381.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  54. SB772077B (SB77) Alleviated the Aqueous Outflow Resistance Mediated by Cyclic Mechanical Stress in Perfused Human Cadaveric Eyes
    Authors: S Ashwinbala, R Haribalaga, S Krishnadas, V Muthukkaru, S Senthilkum
    Sci Rep, 2020-06-23;10(1):10202.
    Species: Human
    Sample Types: Whole Tissues
    Applications: IHC
  55. Acetyl-CoA Carboxylase inhibition improves multiple dimensions of NASH pathogenesis in model systems
    Authors: TT Ross, C Crowley, KL Kelly, A Rinaldi, DA Beebe, MP Lech, RV Martinez, S Carvajal-G, M Boucher, D Hirenallur, J Morin, AC Opsahl, SR Vargas, KK Bence, JA Pfefferkor, WP Esler
    Cell Mol Gastroenterol Hepatol, 2020-06-09;0(0):.
    Species: Rat
    Sample Types: Whole Tissues
    Applications: IHC
  56. Reconstruction of the human blood-brain barrier in vitro reveals a pathogenic mechanism of APOE4 in pericytes
    Authors: JW Blanchard, M Bula, J Davila-Vel, LA Akay, L Zhu, A Frank, MB Victor, JM Bonner, H Mathys, YT Lin, T Ko, DA Bennett, HP Cam, M Kellis, LH Tsai
    Nat. Med., 2020-06-08;26(6):952-963.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  57. Cellular census of human fibrosis defines functionally distinct stromal cell types and states
    Authors: TB Layton, L Williams, F McCann, M Zhang, M Fritzsche, H Colin-York, M Cabrita, MTH Ng, M Feldmann, SN Sansom, D Furniss, W Xie, J Nanchahal
    Nat Commun, 2020-06-02;11(1):2768.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  58. CD8+ T-cell plasticity regulates vascular regeneration in type-2 diabetes
    Authors: C Liang, KY Yang, VW Chan, X Li, THW Fung, Y Wu, XY Tian, Y Huang, L Qin, JYW Lau, KO Lui
    Theranostics, 2020-03-04;10(9):4217-4232.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  59. Dynamic Changes in Function and Proteomic Composition of Extracellular Vesicles from Hepatic Stellate Cells during Cellular Activation
    Authors: X Li, R Chen, S Kemper, DR Brigstock
    Cells, 2020-01-25;9(2):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  60. Generation of Human‐Induced Pluripotent Stem Cells From Anterior Cruciate Ligament
    Authors: Steven Woods, Nicola Bates, Sara L. Dunn, Ferdinand Serracino‐Inglott, Tim E. Hardingham, Susan J. Kimber
    Journal of Orthopaedic Research
  61. Optimal stimulation toward the dermal papilla lineage can be promoted by combined use of osteogenic and adipogenic inducers
    Authors: T Kazi, I Niibe, A Nishikawa, T Matsuzaki
    FEBS Open Bio, 2019-12-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  62. Empagliflozin reduces high glucose-induced oxidative stress and miR-21-dependent TRAF3IP2 induction and RECK suppression, and inhibits human renal proximal tubular epithelial cell migration and epithelial-to-mesenchymal transition
    Authors: NA Das, AJ Carpenter, A Belenchia, AR Aroor, M Noda, U Siebenlist, B Chandrasek, VG DeMarco
    Cell. Signal., 2019-12-17;68(0):109506.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  63. Fibroblast-like synovial cell production of extra domain A fibronectin associates with inflammation in osteoarthritis
    Authors: TW Kragstrup, DH Sohn, CM Lepus, K Onuma, Q Wang, WH Robinson, J Sokolove
    BMC Rheumatol, 2019-12-02;3(0):46.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  64. Adipose Mesenchymal Cells-Derived EVs Alleviate DOCA-Salt-Induced Hypertension by Promoting Cardio-Renal Protection
    Authors: RS Lindoso, JA Lopes, R Binato, E Abdelhay, CM Takiya, KR Miranda, LS Lara, A Viola, B Bussolati, A Vieyra, F Collino
    Mol Ther Methods Clin Dev, 2019-11-15;16(0):63-77.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  65. A new patient‐derived iPSC model for dystroglycanopathies validates a compound that increases glycosylation of alpha ‐dystroglycan
    Authors: Jihee Kim, Beatrice Lana, Silvia Torelli, David Ryan, Francesco Catapano, Pierpaolo Ala et al.
    EMBO reports
  66. Pathological Study on Epithelial-Mesenchymal Transition in Silicotic Lung Lesions in Rat
    Authors: M Komai, K Mihira, A Shimada, I Miyamoto, K Ogihara, Y Naya, T Morita, K Inoue, H Takano
    Vet Sci, 2019-08-30;6(3):.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  67. Myocardin Is Involved in Mesothelial–Mesenchymal Transition of Human Pleural Mesothelial Cells
    Authors: Torry Tucker, Yoshikazu Tsukasaki, Tsuyoshi Sakai, Shinya Mitsuhashi, Satoshi Komatsu, Ann Jeffers et al.
    American Journal of Respiratory Cell and Molecular Biology
  68. Human induced pluripotent stem cell line HMUi001-A derived from corneal stromal cells
    Authors: T Bikkuzin, Y Shi, B Sun, Y Guo, X Jin, Z Han, V Pavlov, H Zhang
    Stem Cell Res, 2019-02-15;37(0):101409.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  69. Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds
    Authors: CF Guerrero-J, PH Dedhia, S Jin, R Ruiz-Vega, D Ma, Y Liu, K Yamaga, O Shestova, DL Gay, Z Yang, K Kessenbroc, Q Nie, WS Pear, G Cotsarelis, MV Plikus
    Nat Commun, 2019-02-08;10(1):650.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  70. Inhibition of PAI-1 limits chemotherapy resistance in lung cancer through suppressing myofibroblast characteristics of cancer-associated fibroblasts
    Authors: T Masuda, T Nakashima, M Namba, K Yamaguchi, S Sakamoto, Y Horimasu, S Miyamoto, H Iwamoto, K Fujitaka, Y Miyata, H Hamada, M Okada, N Hattori
    J. Cell. Mol. Med., 2019-02-07;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  71. TGF-?-SNAIL axis induces M�ller glial-mesenchymal transition in the pathogenesis of idiopathic epiretinal membrane
    Authors: A Kanda, K Noda, I Hirose, S Ishida
    Sci Rep, 2019-01-24;9(1):673.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  72. Small non-coding RNA landscape of extracellular vesicles from human stem cells
    Authors: S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ
    Sci Rep, 2018-10-19;8(1):15503.
  73. Ebola Virus Causes Intestinal Tract Architectural Disruption and Bacterial Invasion in Non-Human Primates
    Authors: RB Reisler, X Zeng, CW Schellhase, JJ Bearss, TK Warren, JC Trefry, GW Christophe, MG Kortepeter, S Bavari, AP Cardile
    Viruses, 2018-09-20;10(10):.
    Applications: IHC-P
  74. Exosomal miR-21-5p derived from gastric cancer promotes peritoneal metastasis via mesothelial-to-mesenchymal transition
    Authors: Q Li, B Li, Q Li, S Wei, Z He, X Huang, L Wang, Y Xia, Z Xu, Z Li, W Wang, L Yang, D Zhang, Z Xu
    Cell Death Dis, 2018-08-28;9(9):854.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  75. Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells
    Authors: J Barnes, F Salas, R Mokhtari, H Dolstra, E Pedrosa, HM Lachman
    Mol Autism, 2018-08-15;9(0):44.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  76. Triiodothyronine alleviates alcoholic liver disease injury through the negative regulation of the NLRP3 signaling pathway
    Authors: Xiang Dong, Hongmei Yang, Cong Li, Qi Liu, Qinglin Bai, Zhaoran Zhang
    Experimental and Therapeutic Medicine
  77. Implication of molecular vascular smooth muscle cell heterogeneity among arterial beds in arterial calcification
    Authors: O Espitia, M Chatelais, M Steenman, C Charrier, B Maurel, S Georges, R Houlgatte, F Verrecchia, B Ory, F Lamoureux, D Heymann, Y Gouëffic, T Quillard
    PLoS ONE, 2018-01-26;13(1):e0191976.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  78. Establishment and characterization of five immortalized human scalp dermal papilla cell lines
    Authors: MH Kwack, JM Yang, GH Won, MK Kim, JC Kim, YK Sung
    Biochem. Biophys. Res. Commun., 2018-01-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  79. Wnt inhibition promotes vascular specification of embryonic cardiac progenitors
    Authors: DE Reichman, L Park, L Man, D Redmond, K Chao, RP Harvey, MM Taketo, Z Rosenwaks, D James
    Development, 2018-01-08;145(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  80. Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-?B and MURF1 levels
    Authors: L Zhou, Y Huang, H Xie, X Mei
    Mol Med Rep, 2017-12-19;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  81. P2X7 receptor antagonism modulates IL-1? and MMP9 in human atherosclerotic vessels
    Authors: M Lombardi, ME Mantione, D Baccellier, D Ferrara, R Castellano, R Chiesa, O Alfieri, C Foglieni
    Sci Rep, 2017-07-07;7(1):4872.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  82. KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis
    Authors: H Kamata, Y Tsukasaki, T Sakai, R Ikebe, J Wang, A Jeffers, J Boren, S Owens, T Suzuki, M Higashihar, S Idell, TA Tucker, M Ikebe
    Sci Rep, 2017-07-04;7(1):4556.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  83. Generation of a human induced pluripotent stem cell line CERAi001-A-6 using episomal vectors
    Authors: RCB Wong, SS Hung, S Jackson, V Singh, S Khan, HH Liang, LS Kearns, T Nguyen, A Conquest, M Daniszewsk, AW Hewitt, A Pébay
    Stem Cell Res, 2017-05-19;22(0):13-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  84. Mitochondrial replacement in an iPSC model of Leber's hereditary optic neuropathy
    Authors: RCB Wong, SY Lim, SSC Hung, S Jackson, S Khan, NJ Van Bergen, E De Smit, HH Liang, LS Kearns, L Clarke, DA Mackey, AW Hewitt, IA Trounce, A Pébay
    Aging (Albany NY), 2017-04-01;9(4):1341-1350.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  85. Cancer-associated mesenchymal stroma fosters the stemness of osteosarcoma cells in response to intratumoral acidosis via NF-?B acivation
    Authors: Sofia Avnet
    Int. J. Cancer, 2017-03-15;0(0):.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC
  86. Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane
    Authors: Y Dong, A Kanda, K Noda, W Saito, S Ishida
    Sci Rep, 2017-03-09;7(0):44266.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  87. NOTCH1-dependent nitric oxide signaling deficiency in hypoplastic left heart syndrome revealed through patient-specific phenotypes detected in bioengineered cardiogenesis
    Authors: SC Hrstka, X Li, TJ Nelson
    Stem Cells, 2017-03-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  88. Cancer-associated fibroblasts promote an immunosuppressive microenvironment through the induction and accumulation of protumoral macrophages
    Authors: H Takahashi, K Sakakura, T Kudo, M Toyoda, K Kaira, T Oyama, K Chikamatsu
    Oncotarget, 2017-01-31;8(5):8633-8647.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  89. Neuropathogenesis of Zika Virus in a Highly Susceptible Immunocompetent Mouse Model after Antibody Blockade of Type I Interferon
    Authors: DR Smith, B Hollidge, S Daye, X Zeng, C Blancett, K Kuszpit, T Bocan, JW Koehler, S Coyne, T Minogue, T Kenny, X Chi, S Yim, L Miller, C Schmaljohn, S Bavari, JW Golden
    PLoS Negl Trop Dis, 2017-01-09;11(1):e0005296.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  90. Human pancreatic cancer cells induce a MyD88-dependent stromal response to promote a tumor-tolerant immune microenvironment
    Cancer Res., 2016-11-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  91. The influence of warm ischemia elimination on kidney injury during transplantation - clinical and molecular study
    Sci Rep, 2016-11-03;6(0):36118.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  92. Generation of Induced Cardiospheres via Reprogramming of Skin Fibroblasts for Myocardial Regeneration
    Authors: Jian-Yong Xu
    Stem Cells, 2016-07-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  93. Exosomes in Human Breast Milk Promote EMT
    Authors: W Qin, Y Tsukasaki, S Dasgupta, N Mukhopadhy, M Ikebe, ER Sauter
    Clin Cancer Res, 2016-04-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  94. miR-142-5p and miR-130a-3p are regulated by IL-4 and IL-13 and control profibrogenic macrophage program
    Authors: Shicheng Su, Qiyi Zhao, Chonghua He, Di Huang, Jiang Liu, Fei Chen et al.
    Nature Communications
  95. Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
    Authors: Riordan D, Varma S, West R, Brown P
    PLoS ONE, 2015-07-15;10(7):e0128975.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  96. CD105 expression on CD34-negative spindle-shaped stromal cells of primary tumor is an unfavorable prognostic marker in early breast cancer patients.
    Authors: Martinez L, Labovsky V, Calcagno M, Davies K, Garcia Rivello H, Bianchi M, Wernicke A, Fernandez Vallone V, Chasseing N
    PLoS ONE, 2015-03-24;10(3):e0121421.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  97. Establishment and characterization of an immortalized human hepatic stellate cell line for applications in co-culturing with immortalized human hepatocytes.
    Authors: Pan X, Wang Y, Yu X, Li J, Zhou N, Du W, Zhang Y, Cao H, Zhu D, Chen Y, Li L
    Int J Med Sci, 2015-02-08;12(3):248-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  98. The bioactivity of composite Fmoc-RGDS-collagen gels
    Authors: Ricardo M. Gouveia, Roanne R. Jones, Ian W. Hamley, Che J. Connon
    Biomater. Sci.
  99. Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability.
    Authors: Davaine J, Quillard T, Brion R, Laperine O, Guyomarch B, Merlini T, Chatelais M, Guilbaud F, Brennan M, Charrier C, Heymann D, Goueffic Y, Heymann M
    PLoS ONE, 2014-09-26;9(9):e107642.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  100. Association between PON1 rs662 polymorphism and coronary artery disease.
    Authors: Liu T, Zhang X, Zhang J, Liang Z, Cai W, Huang M, Yan C, Zhu Z, Han Y
    Eur J Clin Nutr, 2014-06-11;68(9):1029-35.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  101. Efficient generation of integration-free human induced pluripotent stem cells from keratinocytes by simple transfection of episomal vectors.
    Authors: Piao Y, Hung S, Lim S, Wong R, Ko M
    Stem Cells Transl Med, 2014-06-05;3(0):787-791.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  102. Occurrence of tertiary lymphoid tissue is associated with T-cell infiltration and predicts better prognosis in early-stage colorectal cancers.
    Authors: Di Caro G, Bergomas F, Grizzi F, Doni A, Bianchi P, Malesci A, Laghi L, Allavena P, Mantovani A, Marchesi F
    Clin Cancer Res, 2014-02-12;20(8):2147-58.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  103. Modelling the endothelial blood-CNS barriers: a method for the production of robust in vitro models of the rat blood-brain barrier and blood-spinal cord barrier
    Authors: P Marc D Watson, Judy C Paterson, George Thom, Ulrika Ginman, Stefan Lundquist, Carl I Webster
    BMC Neuroscience
  104. Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype.
    Authors: van der Veer E, de Bruin R, Kraaijeveld A, de Vries M, Bot I, Pera T, Segers F, Trompet S, van Gils J, Roeten M, Beckers C, van Santbrink P, Janssen A, van Solingen C, Swildens J, de Boer H, Peters E, Bijkerk R, Rousch M, Doop M, Kuiper J, Schalij M, van der Wal A, Richard S, van Berkel T, Pickering J, Hiemstra P, Goumans M, Rabelink T, de Vries A, Quax P, Jukema J, Biessen E, van Zonneveld A
    Circ Res, 2013-08-20;113(9):1065-75.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  105. Transcriptional effects of 1,25 dihydroxyvitamin D(3) physiological and supra-physiological concentrations in breast cancer organotypic culture.
    Authors: Milani C, Katayama M, de Lyra E, Welsh J, Campos L, Brentani M, Maciel M, Roela R, del Valle P, Goes J, Nonogaki S, Tamura R, Folgueira M
    BMC Cancer, 2013-03-15;13(0):119.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  106. Pharmacological Inhibition of Mitochondrial Carbonic Anhydrases Protects Mouse Cerebral Pericytes from High Glucose-Induced Oxidative Stress and Apoptosis
    Authors: Gul N. Shah, Tulin O. Price, William A. Banks, Yoichi Morofuji, Andrej Kovac, Nuran Ercal et al.
    Journal of Pharmacology and Experimental Therapeutics
  107. Distinct transcriptional profiles of angioblasts derived from human embryonic stem cells.
    Authors: Song S, Jung W, Kim K, Hong W, Kim H, Lee K, Lee K, Suh W
    Exp Cell Res, 2013-02-28;319(8):1136-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  108. Locally different endothelial nitric oxide synthase protein levels in ascending aortic aneurysms of bicuspid and tricuspid aortic valve.
    Authors: Mohamed SA, Radtke A, Saraei R
    Cardiol Res Pract, 2012-06-18;2012(0):165957.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  109. Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study
    Authors: Michael D. Ball, Ian C. Bonzani, Melissa J. Bovis, Andrew Williams, Molly M. Stevens
    Clinical Orthopaedics & Related Research
  110. High-Throughput Tracking of Pluripotent Human Embryonic Stem Cells with Dual Fluorescence Resonance Energy Transfer Molecular Beacons
    Authors: Frank W. King, Walter Liszewski, Carissa Ritner, Harold S. Bernstein
    Stem Cells and Development
  111. An Engineered Cardiac Reporter Cell Line Identifies Human Embryonic Stem Cell-Derived Myocardial Precursors
    Authors: Carissa Ritner, Sharon S. Y. Wong, Frank W. King, Shirley S. Mihardja, Walter Liszewski, David J. Erle et al.
    PLoS ONE
  112. Timed inhibition of p38MAPK directs accelerated differentiation of human embryonic stem cells into cardiomyocytes
    Authors: Meenakshi Gaur, Carissa Ritner, Rich Sievers, Anissa Pedersen, Megha Prasad, Harold S. Bernstein et al.
    Cytotherapy
  113. Subpopulations of Human Embryonic Stem Cells With Distinct Tissue-Specific Fates Can Be Selected From Pluripotent Cultures
    Authors: Frank W. King, Carissa Ritner, Walter Liszewski, Helen C.K. Kwan, Anissa Pedersen, Andrew D. Leavitt et al.
    Stem Cells and Development
  114. Inhibition of caspase-3 differentially affects vascular smooth muscle cell apoptosis in the concave versus convex aortic sites in ascending aneurysms with a bicuspid aortic valve.
    Authors: Mohamed SA, Misfeld M, Hanke T, Charitos EI, Bullerdiek J, Belge G, Kuehnel W, Sievers HH
    Ann. Anat., 2010-04-11;192(3):145-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  115. Effect of an antimicrobial agent on atherosclerotic plaques: assessment of metalloproteinase activity by molecular imaging.
    Authors: Ohshima S, Fujimoto S, Petrov A, Nakagami H, Haider N, Zhou J, Tahara N, Osako MK, Fujimoto A, Zhu J, Murohara T, Edwards DS, Narula N, Wong ND, Chandrashekhar Y, Morishita R, Narula J
    J. Am. Coll. Cardiol., 2010-03-23;55(12):1240-9.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-P
  116. Lysosome-dependent Ca(2+) release response to Fas activation in coronary arterial myocytes through NAADP: evidence from CD38 gene knockouts.
    Authors: Zhang F, Xia M, Li PL
    Am. J. Physiol., Cell Physiol., 2010-03-03;298(5):C1209-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  117. Characterization of a novel embryonic stem cell line from an ICSI-derived blastocyst in the African green monkey
    Authors: Nobuhiro Shimozawa, Shinichiro Nakamura, Ichiro Takahashi, Masanori Hatori, Tadashi Sankai
    REPRODUCTION
  118. The effect of VEGF on the myogenic differentiation of adipose tissue derived stem cells within thermosensitive hydrogel matrices.
    Authors: Kim MH, Hong HN, Hong JP, Park CJ, Kwon SW, Kim SH, Kang G, Kim M
    Biomaterials, 2009-11-14;31(6):1213-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  119. Hypoxia-induced alveolar epithelial-mesenchymal transition requires mitochondrial ROS and hypoxia-inducible factor 1.
    Authors: Zhou G, Dada LA, Wu M, Kelly A, Trejo H, Zhou Q, Varga J, Sznajder JI
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009-10-02;297(6):L1120-30.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  120. Molecular imaging of matrix metalloproteinase in atherosclerotic lesions: resolution with dietary modification and statin therapy.
    Authors: Fujimoto S, Hartung D, Ohshima S, Edwards DS, Zhou J, Yalamanchili P, Azure M, Fujimoto A, Isobe S, Matsumoto Y, Boersma H, Wong N, Yamazaki J, Narula N, Petrov A, Narula J
    J. Am. Coll. Cardiol., 2008-12-02;52(23):1847-57.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  121. Role of matrix metalloprotease-9 in hyperoxic injury in developing lung.
    Authors: Chetty A, Cao GJ, Severgnini M, Simon A, Warburton R, Nielsen HC
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008-07-25;295(4):L584-92.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  122. Breast carcinoma-associated fibroblasts and their counterparts display neoplastic-specific changes.
    Authors: Hawsawi NM, Ghebeh H, Hendrayani SF, Tulbah A, Al-Eid M, Al-Tweigeri T, Ajarim D, Alaiya A, Dermime S, Aboussekhra A
    Cancer Res., 2008-04-15;68(8):2717-25.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  123. Gadodiamide contrast agent 'activates' fibroblasts: a possible cause of nephrogenic systemic fibrosis.
    Authors: Edward M, Quinn JA, Mukherjee S, Jensen MB, Jardine AG, Mark PB, Burden AD
    J. Pathol., 2008-04-01;214(5):584-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  124. Differentiation of human circulating fibrocytes as mediated by transforming growth factor-beta and peroxisome proliferator-activated receptor gamma.
    Authors: Hong KM, Belperio JA, Keane MP, Burdick MD, Strieter RM
    J. Biol. Chem., 2007-06-07;282(31):22910-20.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  125. Bronchoalveolar lavage fluid from patients with acute lung injury/acute respiratory distress syndrome induces myofibroblast differentiation.
    Authors: Synenki L, Chandel NS, Budinger GR, Donnelly HK, Topin J, Eisenbart J, Jovanovic B, Jain M
    Crit. Care Med., 2007-03-01;35(3):842-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  126. A comparative proteomics analysis identified differentially expressed proteins in pancreatic cancer-associated stellate cell small extracellular vesicles
    Authors: Sarcar B, Fang B, Izumi V et al.
    Molecular & Cellular Proteomics
  127. Fibrin polymer on the surface of biomaterial implants drives the foreign body reaction
    Authors: Arnat Balabiyev, Nataly P. Podolnikova, Jacquelyn A. Kilbourne, D. Page Baluch, David Lowry, Azadeh Zare et al.
    Biomaterials

FAQs

  1. Human/Mouse/Rat alpha-Smooth Muscle Actin Antibody, Catalog # MAB1420, is validated for Human/Mouse/Rat IHC, but the datasheet only has Human IHC examples. Was there testing on Mouse and Rat IHC samples?

    • Mouse and Rat samples were tested in Western Blot. We did not perform testing with Mouse or Rat IHC samples, however there are several citations for IHC using Mouse and Rat tissue on our webpage. 

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Reviews for Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

Average Rating: 4.9 (Based on 9 Reviews)

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Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Anonymous on 08/24/2023
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Mesenchymal stem cells Species: Human

Works well at a dilution of 1:500 for IF experiments


Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Anonymous on 10/19/2021
Application: WB Sample Tested: Endothelial cells Species: Mouse

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Anonymous on 08/10/2021
Application: IHC Sample Tested: Testicular tissue Species: Mouse

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Anonymous on 08/04/2021
Application: IHC Sample Tested: Muscle tissue Species: Mouse

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Anonymous on 02/19/2019
Application: IHC Sample Tested: Breast cancer tissue Species: Human

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Anonymous on 07/21/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Anonymous on 12/28/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HMVEC human microvascular endothelial cells Species: Human

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Li Liu on 10/20/2017
Application: WB Sample Tested: Skin tissue Species: Mouse

Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
By Xiaoming Zhou on 10/20/2017
Application: WB Sample Tested: Lung tissue Species: Mouse

The antibody works well for lung tissue of mouse.