Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody

(32 citations)
(2 Reviews)
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human  alpha -Smooth Muscle Actin.
  • Source
    Monoclonal Mouse IgG2A Clone # 1A4
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Peptide corresponding to residues EEEDSTALVC of the N-term of human alpha -smooth muscle actin (1)
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1-0.25 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human alpha ‑Smooth Muscle Actin by Western Blot. Western blot shows lysates of human heart tissue and iBJ6 human induced pluripotent stem cell line undifferentiated or cardiac differentiated. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/
Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha ‑Smooth Muscle Actin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse and Rat alpha ‑Smooth Muscle Actin by Western Blot. Western blot shows lysates of mouse colon tissue, C2C12 mouse myoblast cell line,
NIH‑3T3 mouse embryonic fibroblast cell line, and rat colon tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha ‑Smooth Muscle Actin at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
alpha ‑Smooth Muscle Actin in BG01V Human Embryonic Stem Cells. alpha ‑Smooth Muscle Actin was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiomyocytes using Mouse Anti-Human/Mouse/
Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
alpha ‑Smooth Muscle Actin in Human Heart. alpha ‑Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
alpha -Smooth Muscle Actin in Human Breast Cancer Tissue. alpha -Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/
Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Intracellular Staining by Flow Cytometry
Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of Human
alpha ‑Smooth Muscle Actin by Simple WesternTM.
Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyocytes (+), loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Smooth Muscle Actin at approximately 49 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Smooth Muscle Actin

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

  • References:
    1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
    2. Oishi, K. et al. (2002) J. Physiol. 540:139.
  • Long Name:
    Actin, Alpha 2, Smooth Muscle, Aorta
  • Entrez Gene IDs:
    59 (Human); 11475 (Mouse); 81633 (Rat)
  • Alternate Names:
    AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis
    Authors: H Kamata, Y Tsukasaki, T Sakai, R Ikebe, J Wang, A Jeffers, J Boren, S Owens, T Suzuki, M Higashihar, S Idell, TA Tucker, M Ikebe
    Sci Rep, 2017;7(1):4556.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. P2X7 receptor antagonism modulates IL-1? and MMP9 in human atherosclerotic vessels
    Authors: M Lombardi, ME Mantione, D Baccellier, D Ferrara, R Castellano, R Chiesa, O Alfieri, C Foglieni
    Sci Rep, 2017;7(1):4872.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  3. Mitochondrial replacement in an iPSC model of Leber's hereditary optic neuropathy
    Authors: RCB Wong, SY Lim, SSC Hung, S Jackson, S Khan, NJ Van Bergen, E De Smit, HH Liang, LS Kearns, L Clarke, DA Mackey, AW Hewitt, IA Trounce, A Pébay
    Aging (Albany NY), 2017;9(4):1341-1350.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Cancer-associated fibroblasts promote an immunosuppressive microenvironment through the induction and accumulation of protumoral macrophages
    Authors: H Takahashi, K Sakakura, T Kudo, M Toyoda, K Kaira, T Oyama, K Chikamatsu
    Oncotarget, 2017;8(5):8633-8647.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  5. Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane
    Authors: Y Dong, A Kanda, K Noda, W Saito, S Ishida
    Sci Rep, 2017;7(0):44266.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  6. NOTCH1-dependent nitric oxide signaling deficiency in hypoplastic left heart syndrome revealed through patient-specific phenotypes detected in bioengineered cardiogenesis
    Authors: SC Hrstka, X Li, TJ Nelson,
    Stem Cells, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  7. Neuropathogenesis of Zika Virus in a Highly Susceptible Immunocompetent Mouse Model after Antibody Blockade of Type I Interferon
    Authors: DR Smith, B Hollidge, S Daye, X Zeng, C Blancett, K Kuszpit, T Bocan, JW Koehler, S Coyne, T Minogue, T Kenny, X Chi, S Yim, L Miller, C Schmaljohn, S Bavari, JW Golden
    PLoS Negl Trop Dis, 2017;11(1):e0005296.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  8. Generation of Induced Cardiospheres via Reprogramming of Skin Fibroblasts for Myocardial Regeneration
    Authors: Jian-Yong Xu
    Stem Cells, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC - PFA fixed
  9. The influence of warm ischemia elimination on kidney injury during transplantation - clinical and molecular study
    Sci Rep, 2016;6(0):36118.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  10. Human pancreatic cancer cells induce a MyD88-dependent stromal response to promote a tumor-tolerant immune microenvironment
    Cancer Res., 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Exosomes in Human Breast Milk Promote EMT
    Authors: W Qin, Y Tsukasaki, S Dasgupta, N Mukhopadhy, M Ikebe, ER Sauter
    Clin Cancer Res, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: IF
  12. Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
    Authors: Riordan D, Varma S, West R, Brown P
    PLoS ONE, 2015;10(7):e0128975.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  13. CD105 expression on CD34-negative spindle-shaped stromal cells of primary tumor is an unfavorable prognostic marker in early breast cancer patients.
    Authors: Martinez L, Labovsky V, Calcagno M, Davies K, Garcia Rivello H, Bianchi M, Wernicke A, Fernandez Vallone V, Chasseing N
    PLoS ONE, 2015;10(3):e0121421.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  14. Association between PON1 rs662 polymorphism and coronary artery disease.
    Authors: Liu T, Zhang X, Zhang J, Liang Z, Cai W, Huang M, Yan C, Zhu Z, Han Y
    Eur J Clin Nutr, 2014;68(9):1029-35.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - paraffin
  15. Occurrence of tertiary lymphoid tissue is associated with T-cell infiltration and predicts better prognosis in early-stage colorectal cancers.
    Authors: Di Caro G, Bergomas F, Grizzi F, Doni A, Bianchi P, Malesci A, Laghi L, Allavena P, Mantovani A, Marchesi F
    Clin Cancer Res, 2014;20(8):2147-58.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  16. Efficient generation of integration-free human induced pluripotent stem cells from keratinocytes by simple transfection of episomal vectors.
    Authors: Piao Y, Hung S, Lim S, Wong R, Ko M
    Stem Cells Transl Med, 2014;3(0):787-791.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  17. Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability.
    Authors: Davaine J, Quillard T, Brion R, Laperine O, Guyomarch B, Merlini T, Chatelais M, Guilbaud F, Brennan M, Charrier C, Heymann D, Goueffic Y, Heymann M
    PLoS ONE, 2014;9(9):e107642.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  18. Transcriptional effects of 1,25 dihydroxyvitamin D(3) physiological and supra-physiological concentrations in breast cancer organotypic culture.
    Authors: Milani C, Katayama M, de Lyra E, Welsh J, Campos L, Brentani M, Maciel M, Roela R, del Valle P, Goes J, Nonogaki S, Tamura R, Folgueira M
    BMC Cancer, 2013;13(0):119.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  19. Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype.
    Authors: van der Veer E, de Bruin R, Kraaijeveld A, de Vries M, Bot I, Pera T, Segers F, Trompet S, van Gils J, Roeten M, Beckers C, van Santbrink P, Janssen A, van Solingen C, Swildens J, de Boer H, Peters E, Bijkerk R, Rousch M, Doop M, Kuiper J, Schalij M, van der Wal A, Richard S, van Berkel T, Pickering J, Hiemstra P, Goumans M, Rabelink T, de Vries A, Quax P, Jukema J, Biessen E, van Zonneveld A
    Circ Res, 2013;113(9):1065-75.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  20. Distinct transcriptional profiles of angioblasts derived from human embryonic stem cells.
    Authors: Song S, Jung W, Kim K, Hong W, Kim H, Lee K, Lee K, Suh W
    Exp Cell Res, 2013;319(8):1136-45.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  21. Locally different endothelial nitric oxide synthase protein levels in ascending aortic aneurysms of bicuspid and tricuspid aortic valve.
    Authors: Mohamed SA, Radtke A, Saraei R
    Cardiol Res Pract, 2012;2012(0):165957.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  22. Inhibition of caspase-3 differentially affects vascular smooth muscle cell apoptosis in the concave versus convex aortic sites in ascending aneurysms with a bicuspid aortic valve.
    Authors: Mohamed SA, Misfeld M, Hanke T, Charitos EI, Bullerdiek J, Belge G, Kuehnel W, Sievers HH
    Ann. Anat., 2010;192(3):145-50.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  23. Lysosome-dependent Ca(2+) release response to Fas activation in coronary arterial myocytes through NAADP: evidence from CD38 gene knockouts.
    Authors: Zhang F, Xia M, Li PL
    Am. J. Physiol., Cell Physiol., 2010;298(5):C1209-16.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  24. Effect of an antimicrobial agent on atherosclerotic plaques: assessment of metalloproteinase activity by molecular imaging.
    Authors: Ohshima S, Fujimoto S, Petrov A, Nakagami H, Haider N, Zhou J, Tahara N, Osako MK, Fujimoto A, Zhu J, Murohara T, Edwards DS, Narula N, Wong ND, Chandrashekhar Y, Morishita R, Narula J
    J. Am. Coll. Cardiol., 2010;55(12):1240-9.
    Species: Rabbit
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  25. The effect of VEGF on the myogenic differentiation of adipose tissue derived stem cells within thermosensitive hydrogel matrices.
    Authors: Kim MH, Hong HN, Hong JP, Park CJ, Kwon SW, Kim SH, Kang G, Kim M
    Biomaterials, 2010;31(6):1213-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  26. Hypoxia-induced alveolar epithelial-mesenchymal transition requires mitochondrial ROS and hypoxia-inducible factor 1.
    Authors: Zhou G, Dada LA, Wu M, Kelly A, Trejo H, Zhou Q, Varga J, Sznajder JI
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(6):L1120-30.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  27. Molecular imaging of matrix metalloproteinase in atherosclerotic lesions: resolution with dietary modification and statin therapy.
    Authors: Fujimoto S, Hartung D, Ohshima S, Edwards DS, Zhou J, Yalamanchili P, Azure M, Fujimoto A, Isobe S, Matsumoto Y, Boersma H, Wong N, Yamazaki J, Narula N, Petrov A, Narula J
    J. Am. Coll. Cardiol., 2008;52(23):1847-57.
    Species: Rabbit
    Sample Type: Whole Tissue
    Application: IHC
  28. Gadodiamide contrast agent 'activates' fibroblasts: a possible cause of nephrogenic systemic fibrosis.
    Authors: Edward M, Quinn JA, Mukherjee S, Jensen MB, Jardine AG, Mark PB, Burden AD
    J. Pathol., 2008;214(5):584-93.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  29. Breast carcinoma-associated fibroblasts and their counterparts display neoplastic-specific changes.
    Authors: Hawsawi NM, Ghebeh H, Hendrayani SF, Tulbah A, Al-Eid M, Al-Tweigeri T, Ajarim D, Alaiya A, Dermime S, Aboussekhra A
    Cancer Res., 2008;68(8):2717-25.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  30. Role of matrix metalloprotease-9 in hyperoxic injury in developing lung.
    Authors: Chetty A, Cao GJ, Severgnini M, Simon A, Warburton R, Nielsen HC
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008;295(4):L584-92.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  31. Bronchoalveolar lavage fluid from patients with acute lung injury/acute respiratory distress syndrome induces myofibroblast differentiation.
    Authors: Synenki L, Chandel NS, Budinger GR, Donnelly HK, Topin J, Eisenbart J, Jovanovic B, Jain M
    Crit. Care Med., 2007;35(3):842-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  32. Differentiation of human circulating fibrocytes as mediated by transforming growth factor-beta and peroxisome proliferator-activated receptor gamma.
    Authors: Hong KM, Belperio JA, Keane MP, Burdick MD, Strieter RM
    J. Biol. Chem., 2007;282(31):22910-20.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Expand to show all 32 Citations
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Average Rating: 5 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Mouse.
We have 2 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human/Mouse/Rat  alpha -Smooth Muscle Actin Antibody MAB1420
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Excellent
 WB Mouse Anonymous 10/20/2017
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Western Blot Human/Mouse/Rat  alpha -Smooth Muscle Actin Antibody MAB1420
Western Blot: Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody [MAB1420]

Summary

ApplicationWestern Blot
Sample TestedSkin tissue
SpeciesMouse
Western Blot Human/Mouse/Rat  alpha -Smooth Muscle Actin Antibody MAB1420
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Excellent
 WB Mouse Anonymous 10/20/2017
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Western Blot Human/Mouse/Rat  alpha -Smooth Muscle Actin Antibody MAB1420
Western Blot: Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody [MAB1420]

Summary

ApplicationWestern Blot
Sample TestedLung tissue
SpeciesMouse

Other Experimental Details

Other Experimental DetailsThe antibody works well for lung tissue of mouse.

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