Intracellular Staining by Flow Cytometry
Detection of alpha ‑Smooth Muscle Actin in Human PBMC Monocytes by Flow Cytometry.Human peripheral blood mononuclear cell (PBMC) Monocytes were stained with Mouse Anti-Human
alpha ‑Smooth Muscle Actin Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # IC1420T, filled histogram) or isotype control antibody (Catalog # IC003T, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided