Human alpha-Smooth Muscle Actin Alexa Fluor® 594-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of alpha ‑Smooth Muscle Actin in Human PBMC Monocytes by Flow Cytometry.
Human peripheral blood mononuclear cell (PBMC) Monocytes were stained with Mouse Anti-Human
alpha ‑Smooth Muscle Actin Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # IC1420T, filled histogram) or isotype control antibody (Catalog # IC003T, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: alpha-Smooth Muscle Actin
alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
- Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
- Oishi, K. et al. (2002) J. Physiol. 540:139.
Citations for Human alpha-Smooth Muscle Actin Alexa Fluor® 594-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Dissecting Effects of Anti-cancer Drugs and Cancer-Associated Fibroblasts by On-Chip Reconstitution of Immunocompetent Tumor Microenvironments
Authors: M Nguyen, A De Ninno, A Mencattini, F Mermet-Mei, G Fornabaio, SS Evans, M Cossutta, Y Khira, W Han, P Sirven, F Pelon, D Di Giusepp, FR Bertani, A Gerardino, A Yamada, S Descroix, V Soumelis, F Mechta-Gri, G Zalcman, J Camonis, E Martinelli, L Businaro, MC Parrini
Cell Rep, 2018;25(13):3884-3893.e3.
Sample Types: Whole Cells
Applications: Flow Cytometry
Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer
Authors: A Costa, Y Kieffer, A Scholer-Da, F Pelon, B Bourachot, M Cardon, P Sirven, I Magagna, L Fuhrmann, C Bernard, C Bonneau, M Kondratova, I Kuperstein, A Zinovyev, AM Givel, MC Parrini, V Soumelis, A Vincent-Sa, F Mechta-Gri
Cancer Cell, 2018;0(0):.
Sample Types: Whole Cells
Applications: Cell Culture
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