Detects human alpha -Smooth Muscle Actin.
Monoclonal Mouse IgG
2A Clone # 1A4
Protein A or G purified from hybridoma culture supernatant
alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 594
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Intracellular Staining by Flow Cytometry
Human peripheral blood mononuclear cell (PBMC) Monocytes were stained with Mouse Anti-Human
Detection of alpha ‑Smooth Muscle Actin in Human PBMC Monocytes by Flow Cytometry.
alpha ‑Smooth Muscle Actin Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # IC1420T, filled histogram) or isotype control antibody (Catalog # IC003T, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Background: alpha-Smooth Muscle Actin
alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
et al. (1986) J. Cell Biol. 103:2787. Oishi, K. et al. (2002) J. Physiol. 540:139.
Actin, Alpha 2, Smooth Muscle, Aorta
Entrez Gene IDs:
59 (Human); 11475 (Mouse); 81633 (Rat)
AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin
Related Research Areas
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