Human alpha-Smooth Muscle Actin Alexa Fluor® 594-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of alpha ‑Smooth Muscle Actin in Human PBMC Monocytes by Flow Cytometry.
Human peripheral blood mononuclear cell (PBMC) Monocytes were stained with Mouse Anti-Human
alpha ‑Smooth Muscle Actin Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # IC1420T, filled histogram) or isotype control antibody (Catalog # IC003T, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: alpha-Smooth Muscle Actin
alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
- Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
- Oishi, K. et al. (2002) J. Physiol. 540:139.
Citation for Human alpha-Smooth Muscle Actin Alexa Fluor® 594-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Dissecting Effects of Anti-cancer Drugs and Cancer-Associated Fibroblasts by On-Chip Reconstitution of Immunocompetent Tumor Microenvironments
Authors: M Nguyen, A De Ninno, A Mencattini, F Mermet-Mei, G Fornabaio, SS Evans, M Cossutta, Y Khira, W Han, P Sirven, F Pelon, D Di Giusepp, FR Bertani, A Gerardino, A Yamada, S Descroix, V Soumelis, F Mechta-Gri, G Zalcman, J Camonis, E Martinelli, L Businaro, MC Parrini
Cell Rep, 2018;25(13):3884-3893.e3.
Sample Types: Whole Cells
Applications: Flow Cytometry
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human alpha-Smooth Muscle Actin Alexa Fluor® 594-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human alpha-Smooth Muscle Actin Alexa Fluor® 594-conjugated Antibody and earn rewards!
Have you used Human alpha-Smooth Muscle Actin Alexa Fluor® 594-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image