Human alpha-Smooth Muscle Actin PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC1420P
Detection of  alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry.
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Citations (14)
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Human alpha-Smooth Muscle Actin PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human  alpha -Smooth Muscle Actin.
Source
Monoclonal Mouse IgG2A Clone # 1A4
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

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Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of  alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. View Larger

Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human a-Smooth Muscle Actin PE-conjugated Monoclonal Antibody (Catalog # IC1420P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: alpha-Smooth Muscle Actin

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

References
  1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
  2. Oishi, K. et al. (2002) J. Physiol. 540:139.
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Entrez Gene IDs
59 (Human); 11475 (Mouse); 81633 (Rat)
Alternate Names
AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin

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Citations for Human alpha-Smooth Muscle Actin PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Central Role of Dendritic Cells in Pulmonary Arterial Hypertension in Human and Mice
    Authors: D van Uden, T Koudstaal, JAC van Hulst, IM Bergen, C Gootjes, NW Morrell, G van Loo, JH von der Th, TPP van den Bo, MR Ghigna, F Perros, D Montani, M Kool, KA Boomars, RW Hendriks
    International Journal of Molecular Sciences, 2021;22(4):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Induced osteogenic differentiation of human smooth muscle cells as a model of vascular calcification
    Authors: W Pustlauk, TH Westhoff, L Claeys, T Roch, S Gei beta ler, N Babel
    Sci Rep, 2020;10(1):5951.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Human Liver Memory CD8+ T Cells Use Autophagy for Tissue Residence
    Authors: L Swadling, LJ Pallett, MO Diniz, JM Baker, OE Amin, KA Stegmann, AR Burton, NM Schmidt, A Jeffery-Sm, N Zakeri, K Suveizdyte, F Froghi, G Fusai, WM Rosenberg, BR Davidson, A Schurich, AK Simon, MK Maini
    Cell Rep, 2020;30(3):687-698.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A novel flow cytometry tool for fibrosis scoring through hepatic stellate cell differentiation
    Authors: J Amer, A Salhab, S Doron, G Morali, R Safadi
    Cytometry A, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The Role of the RhoA/ROCK Signaling Pathway in Mechanical Strain-Induced Scleral Myofibroblast Differentiation
    Authors: Y Yuan, M Li, CH To, TC Lam, P Wang, Y Yu, Q Chen, X Hu, B Ke
    Invest. Ophthalmol. Vis. Sci., 2018;59(8):3619-3629.
    Species: Guinea Pig
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Attenuated PDGF signaling drives alveolar and microvascular defects in neonatal chronic lung�disease
    Authors: P Oak, T Pritzke, I Thiel, M Koschlig, DS Mous, A Windhorst, N Jain, O Eickelberg, K Foerster, A Schulze, W Goepel, T Reicherzer, H Ehrhardt, RJ Rottier, P Ahnert, L Gortner, TJ Desai, A Hilgendorf
    EMBO Mol Med, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Reduced suppressive effect of ?2-adrenoceptor agonist on fibrocyte function in severe asthma
    Authors: CY Lo, C Michaeloud, PK Bhavsar, CD Huang, PJ Chang, CH Wang, HP Kuo, KF Chung
    Respir. Res., 2017;18(1):194.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar.
    Authors: Kwan P, Ding J, Tredget E
    PLoS ONE, 2015;10(4):e0123054.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015;33(1):133-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Natural killer cell-dependent anti-fibrotic pathway in liver injury via Toll-like receptor-9.
    Authors: Abu-Tair L, Axelrod J, Doron S, Ovadya Y, Krizhanovsky V, Galun E, Amer J, Safadi R
    PLoS ONE, 2013;8(12):e82571.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Loss of endogenous bone morphogenetic protein-6 aggravates renal fibrosis.
    Authors: Dendooven A, van Oostrom O, van der Giezen DM, Leeuwis JW, Snijckers C, Joles JA, Robertson EJ, Verhaar MC, Nguyen TQ, Goldschmeding R
    Am. J. Pathol., 2011;178(3):1069-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Circulating angiogenic precursors in idiopathic pulmonary arterial hypertension.
    Authors: Asosingh K, Aldred MA, Vasanji A, Drazba J, Sharp J, Farver C, Comhair SA, Xu W, Licina L, Huang L, Anand-Apte B, Yoder MC, Tuder RM, Erzurum SC
    Am. J. Pathol., 2008;172(3):615-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells.
    Authors: Nazareth MR, Broderick L, Simpson-Abelson MR, Kelleher RJ, Yokota SJ, Bankert RB
    J. Immunol., 2007;178(9):5552-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
    Authors: Semenov O, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch A, Malek A
    Am J Obstet Gynecol, 0;202(2):193.e1-193.e1.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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