Human alpha -Smooth Muscle Actin PE-conjugated Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human  alpha -Smooth Muscle Actin.
  • Source
    Monoclonal Mouse IgG2A Clone # 1A4
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human alpha ‑Smooth Muscle Actin PE‑conjugated Monoclonal Antibody (Catalog # IC1420P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: alpha-Smooth Muscle Actin
alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
  • References:
    1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
    2. Oishi, K. et al. (2002) J. Physiol. 540:139.
  • Long Name:
    Actin, Alpha 2, Smooth Muscle, Aorta
  • Entrez Gene IDs:
    59 (Human); 11475 (Mouse); 81633 (Rat)
  • Alternate Names:
    AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar.
    Authors: Kwan P, Ding J, Tredget E
    PLoS ONE, 2015;10(4):e0123054.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015;33(1):133-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Natural killer cell-dependent anti-fibrotic pathway in liver injury via Toll-like receptor-9.
    Authors: Abu-Tair L, Axelrod J, Doron S, Ovadya Y, Krizhanovsky V, Galun E, Amer J, Safadi R
    PLoS ONE, 2013;8(12):e82571.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Loss of endogenous bone morphogenetic protein-6 aggravates renal fibrosis.
    Authors: Dendooven A, van Oostrom O, van der Giezen DM, Leeuwis JW, Snijckers C, Joles JA, Robertson EJ, Verhaar MC, Nguyen TQ, Goldschmeding R
    Am. J. Pathol., 2011;178(3):1069-79.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Circulating angiogenic precursors in idiopathic pulmonary arterial hypertension.
    Authors: Asosingh K, Aldred MA, Vasanji A, Drazba J, Sharp J, Farver C, Comhair SA, Xu W, Licina L, Huang L, Anand-Apte B, Yoder MC, Tuder RM, Erzurum SC
    Am. J. Pathol., 2008;172(3):615-27.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells.
    Authors: Nazareth MR, Broderick L, Simpson-Abelson MR, Kelleher RJ, Yokota SJ, Bankert RB
    J. Immunol., 2007;178(9):5552-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 13  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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