Human ASK1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human ASK1 by Western Blot.
1 Image
Product Details
Citations (2)
Supplemental Products
Reviews (1)

Human ASK1 Antibody Summary

Species Reactivity
Detects human ASK1 in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human ASK1
Accession # Q99683
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
Immersion fixed human peripheral blood mononuclear cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ASK1 antibody by Western Blot. View Larger

Detection of Human ASK1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ASK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3575) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ASK1 at approximately 154 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASK1

Apoptosis signal-regulating kinase 1 (ASK1) is a MAP kinase kinase kinase also known as MEKK5 and MAP3K5. ASK1 is induced by inflammatory cytokines, UV light, and other stress-related stimuli to phosphorylate MAP kinase kinases that in turn activate the p38 and JNK families of MAP kinases. Mice lacking ASK1 are resistant to stress-induced p38 and JNK activation and cell death.

Long Name
Apoptosis Signal-regulated Kinase 1
Entrez Gene IDs
4217 (Human); 26408 (Mouse)
Alternate Names
Apoptosis signal-regulating kinase 1; ASK1; ASK-1; ASK1MEKK 5; EC 2.7.11; MAP/ERK kinase kinase 5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5EC; MEK kinase 5; MEKK5; MEKK5apoptosis signal regulating kinase 1; mitogen-activated protein kinase kinase kinase 5

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Citations for Human ASK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Semaphorin7A regulates neuroglial plasticity in the adult hypothalamic median eminence.
    Authors: Parkash J, Messina A, Langlet F, Cimino I, Loyens A, Mazur D, Gallet S, Balland E, Malone S, Pralong F, Cagnoni G, Schellino R, De Marchis S, Mazzone M, Pasterkamp R, Tamagnone L, Prevot V, Giacobini P
    Nat Commun, 2015;6(0):6385.
  2. Pre-exposure of Mycobacterium tuberculosis-infected macrophages to crystalline silica impairs control of bacterial growth by deregulating the balance between apoptosis and necrosis.
    Authors: Chavez-Galan L, Ramon-Luing L, Torre-Bouscoulet L, Perez-Padilla R, Sada-Ovalle I
    PLoS ONE, 2013;8(11):e80971.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Human ASK1 Antibody
By Anonymous on 01/25/2018
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human