Human ASK1 Antibody

  (2 citations)
(1 Review)
    
Datasheet / CoA / SDS
Product Details
Citations (2)
FAQs
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  • Species Reactivity
    Human
  • Specificity
    Detects human ASK1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human ASK1
    Lys1011-Asp1196
    Accession # Q99683
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed human peripheral blood mononuclear cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Human ASK1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ASK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3575) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ASK1 at approximately 154 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ASK1

Apoptosis signal-regulating kinase 1 (ASK1) is a MAP kinase kinase kinase also known as MEKK5 and MAP3K5. ASK1 is induced by inflammatory cytokines, UV light, and other stress-related stimuli to phosphorylate MAP kinase kinases that in turn activate the p38 and JNK families of MAP kinases. Mice lacking ASK1 are resistant to stress-induced p38 and JNK activation and cell death.

  • Long Name:
    Apoptosis Signal-regulated Kinase 1
  • Entrez Gene IDs:
    4217 (Human); 26408 (Mouse)
  • Alternate Names:
    Apoptosis signal-regulating kinase 1; ASK1; ASK-1; ASK1MEKK 5; EC 2.7.11; MAP/ERK kinase kinase 5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5EC 2.7.11.25; MEK kinase 5; MEKK5; MEKK5apoptosis signal regulating kinase 1; mitogen-activated protein kinase kinase kinase 5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Applications
Sample Type
  1. Semaphorin7A regulates neuroglial plasticity in the adult hypothalamic median eminence.
    Authors: Parkash J, Messina A, Langlet F, Cimino I, Loyens A, Mazur D, Gallet S, Balland E, Malone S, Pralong F, Cagnoni G, Schellino R, De Marchis S, Mazzone M, Pasterkamp R, Tamagnone L, Prevot V, Giacobini P
    Nat Commun, 2015;6(0):6385.
  2. Pre-exposure of Mycobacterium tuberculosis-infected macrophages to crystalline silica impairs control of bacterial growth by deregulating the balance between apoptosis and necrosis.
    Authors: Chavez-Galan L, Ramon-Luing L, Torre-Bouscoulet L, Perez-Padilla R, Sada-Ovalle I
    PLoS ONE, 2013;8(11):e80971.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

FAQs

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Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

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Isotype Controls
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

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Secondary Antibodies
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Sheep IgG (H+L) APC-conjugated Antibody

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Sheep IgG VisUCyte HRP Polymer Antibody

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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human ASK1 Antibody AF3575 Array AF3575
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Very Good
 WB Human Anonymous 01/25/2018
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Western Blot Human ASK1 Antibody AF3575
Western Blot: Human ASK1 Antibody [AF3575].

Summary

Application Western Blot
Sample Tested MDA-MB-231 human breast cancer cell line
Species Human

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