Human Aurora A Biotinylated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Aurora A in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) Aurora B and rhAurora C is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Aurora A
    Met1-Glu131
    Accession # O14965
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Aurora A
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Immunocytochemistry
Aurora A in HeLa Human Cell Line. Aurora A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Aurora A Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3295) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI(blue). Specific staining was localized to mitotic spindles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aurora A
Aurora A is a serine/threonine kinase that regulates chromosome segregation and cytokinesis during mitosis. Its kinase activity is thought to regulate the G2 to M transition of the cell cycle by phosphorylating BRCA1. Aberrant expression and activity of Aurora A in tumors, including breast, ovarian, gastric and colorectal cancer, suggests that it plays a role in tumor development and progression.
  • Entrez Gene IDs:
    6790 (Human); 20878 (Mouse)
  • Alternate Names:
    AIK; AIKAurA; ARK1; ARK-1; ARK1EC 2.7.11.1; AURA; AURKA; Aurora A; aurora kinase AhARK1; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; Aurora-related kinase 1; Breast tumor-amplified kinase; breast-tumor-amplified kinase; BTAK; BTAKAURORA2; EC 2.7.11; IPL1-related kinase; serine/threonine kinase 6; serine/threonine protein kinase 15; Serine/threonine-protein kinase 15; serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; STK15; STK15serine/threonine kinase 15; STK6; STK6MGC34538; STK7
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 4
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Streptavidin-Phycoerythrin

Flow F0040 7
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NorthernLights Streptavidin NL557

Flow , IHC , ICC NL999 4
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Streptavidin-Allophycocyanin

Flow F0050 1
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NorthernLights Streptavidin NL493

Flow , IHC , ICC NL997 2
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NorthernLights Streptavidin NL637

Flow , IHC , ICC NL998 2
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 51  
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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