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Human Aurora A Antibody

Catalog #: AF3295
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3295
AF3295-SP

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Detection of Human Aurora A by Western Blot.
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Product Details
Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Aurora A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Aurora A in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Aurora A
Met1-Glu131
Accession # O14965
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
12.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Aurora A antibody by Western Blot. View Larger

Detection of Human Aurora A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Human Aurora A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3295) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Aurora A at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Simple Western Detection of Human Aurora A antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Aurora A by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Aurora A at approximately 56 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human Aurora A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3295) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aurora A

Aurora A is a serine/threonine kinase that regulates chromosome segregation and cytokinesis during mitosis. Its kinase activity is thought to regulate the G2 to M transition of the cell cycle by phosphorylating BRCA1. Aberrant expression and activity of Aurora A in tumors, including breast, ovarian, gastric and colorectal cancer, suggests that it plays a role in tumor development and progression.

Entrez Gene IDs
6790 (Human); 20878 (Mouse)
Alternate Names
AIK; AIKAurA; ARK1; ARK-1; ARK1EC 2.7.11.1; AURA; AURKA; Aurora A; aurora kinase AhARK1; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; Aurora-related kinase 1; Breast tumor-amplified kinase; breast-tumor-amplified kinase; BTAK; BTAKAURORA2; EC 2.7.11; IPL1-related kinase; serine/threonine kinase 6; serine/threonine protein kinase 15; Serine/threonine-protein kinase 15; serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; STK15; STK15serine/threonine kinase 15; STK6; STK6MGC34538; STK7

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Citations for Human Aurora A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. The Genomic Landscape of Intrinsic and Acquired Resistance to Cyclin-Dependent Kinase 4/6 Inhibitors in Patients with Hormone Receptor–Positive Metastatic Breast Cancer
    Authors: Seth A. Wander, Ofir Cohen, Xueqian Gong, Gabriela N. Johnson, Jorge E. Buendia-Buendia, Maxwell R. Lloyd et al.
    Cancer Discovery
  2. Tamoxifen Rechallenge Decreases Metastatic Potential but Increases Cell Viability and Clonogenicity in a Tamoxifen-Mediated Cytotoxicity-Resistant Subline of Human Breast MCF7 Cancer Cells
    Authors: Yung-Chieh Chang, Chun Hei Antonio Cheung, Yao-Lung Kuo
    Frontiers in Cell and Developmental Biology
  3. Aurora A selective inhibitor LY3295668 leads to dominant mitotic arrest, apoptosis in cancer cells and shows potent preclinical antitumor efficacy
    Authors: J Du, L Yan, R Torres, X Gong, H Bian, C Marugán, K Boehnke, C Baquero, YH Hui, SC Chapman, Y Yang, Y Zeng, SM Bogner, RT Foreman, A Capen, GP Donoho, RD Van Horn, DS Barnard, JA Dempsey, RP Beckmann, MS Marshall, LC Chio, Y Qian, YW Webster, A Aggarwal, S Chu, S Bhattachar, LF Stancato, MS Dowless, PW Iversen, JR Manro, JL Walgren, BW Halstead, MZ Dieter, R Martinez, SV Bhagwat, EL Kreklau, MJ Lallena, XS Ye, BKR Patel, C Reinhard, GD Plowman, DA Barda, JR Henry, SG Buchanan, RM Campbell
    Mol. Cancer Ther., 2019-09-17;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Western Blot
  4. Aurora-A kinase inhibition is synthetic lethal with loss of the RB1 tumor suppressor gene
    Authors: X Gong, J Du, SH Parsons, FF Merzoug, Y Webster, PW Iversen, LC Chio, RD Van Horn, X Lin, W Blosser, B Han, S Jin, S Yao, H Bian, C Ficklin, L Fan, A Kapoor, S Antonysamy, AM Mc Nulty, K Froning, D Manglicmot, A Pustilnik, K Weichert, SR Wasserman, M Dowless, C Marugán, C Baquero, MJ Lallena, SW Eastman, YH Hui, MZ Dieter, T Doman, S Chu, HR Qian, XS Ye, DA Barda, GD Plowman, C Reinhard, RM Campbell, JR Henry, SG Buchanan
    Cancer Discov, 2018-10-29;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Kinome-wide RNAi studies in human multiple myeloma identify vulnerable kinase targets, including a lymphoid-restricted kinase, GRK6.
    Authors: Tiedemann RE, Zhu YX, Schmidt J, Yin H, Shi CX, Que Q, Basu G, Azorsa D, Perkins LM, Braggio E, Fonseca R, Bergsagel PL, Mousses S, Stewart AK
    Blood, 2009-12-07;115(8):1594-604.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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