Human Aurora A Antibody Summary
Accession # O14965
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Aurora A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Human Aurora A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3295) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Aurora A at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Detection of Human Aurora A by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Aurora A at approximately 56 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human Aurora A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3295) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aurora A
Aurora A is a serine/threonine kinase that regulates chromosome segregation and cytokinesis during mitosis. Its kinase activity is thought to regulate the G2 to M transition of the cell cycle by phosphorylating BRCA1. Aberrant expression and activity of Aurora A in tumors, including breast, ovarian, gastric and colorectal cancer, suggests that it plays a role in tumor development and progression.
Citations for Human Aurora A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
Aurora-A kinase inhibition is synthetic lethal with loss of the RB1 tumor suppressor gene
Authors: X Gong, J Du, SH Parsons, FF Merzoug, Y Webster, PW Iversen, LC Chio, RD Van Horn, X Lin, W Blosser, B Han, S Jin, S Yao, H Bian, C Ficklin, L Fan, A Kapoor, S Antonysamy, AM Mc Nulty, K Froning, D Manglicmot, A Pustilnik, K Weichert, SR Wasserman, M Dowless, C Marugán, C Baquero, MJ Lallena, SW Eastman, YH Hui, MZ Dieter, T Doman, S Chu, HR Qian, XS Ye, DA Barda, GD Plowman, C Reinhard, RM Campbell, JR Henry, SG Buchanan
Cancer Discov, 2018;0(0):.
Sample Types: Cell Lysate
Kinome-wide RNAi studies in human multiple myeloma identify vulnerable kinase targets, including a lymphoid-restricted kinase, GRK6.
Authors: Tiedemann RE, Zhu YX, Schmidt J, Yin H, Shi CX, Que Q, Basu G, Azorsa D, Perkins LM, Braggio E, Fonseca R, Bergsagel PL, Mousses S, Stewart AK
Sample Types: Cell Lysates
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Aurora A Antibody
There are currently no reviews for this product. Be the first to review Human Aurora A Antibody and earn rewards!
Have you used Human Aurora A Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image