Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Cell Culture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 409633
View all BAI3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BAI3
Ala25-Thr880
Accession # O60242

Specificity

Detects human BAI3 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human BAI3 Antibody

Detection of BAI3 antibody in HEK293 Human Cell Line Transfected with Human BAI3 and eGFP antibody by Flow Cytometry

Detection of BAI3 in HEK293 Human Cell Line Transfected with Human BAI3 and eGFP by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with either (A) human BAI3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human BAI3 Monoclonal Antibody (Catalog # MAB39651) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
Detection of BAI3 antibody in SHSY-5Y Human Neuroblastoma Cell Line

Detection of BAI3 in SHSY-5Y Human Neuroblastoma Cell Line

SHSY-5Y human neuroblastoma cell line was stained with Mouse Anti-Human BAI3 Monoclonal Antibody (Catalog # MAB39651, filled histogram) or Mouse IgG1 Isotype Control Antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human BAI3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human BAI3 and eGFP and SHSY-5Y Human Neuroblastoma Cell Line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BAI3

Human BAI3 (brain-specific angiogenesis inhibitor 3) is a 177 kDa, 7-transmembrane (TM) member of the secretin receptor family. It is synthesized by neurons of the CNS and likely is a negative regulator of angiogenesis. BAI3 is 1498 amino acids (aa) in size. It contains three distinct regions; an N-terminal extracellular domain (ECD) (aa 25‑883), a 7-TM segment, and a C-terminal cytoplasmic region. The ECD contains four antiangiogenic TSP type1 repeats (aa 296‑508), and one GPS domain (aa 816‑867) that is likely used to cleave the ECD from the membrane-bound receptor. There is one alternate splice form that shows a deletion of aa 643‑665. Over aa 25‑880, human BAI3 shares 98% aa identity with mouse BAI3.

Long Name

Brain-specific Angiogenesis Inhibitor 3

Alternate Names

brain-specific angiogenesis inhibitor 3, dJ91B17.1 (brain-specific angiogenesis inhibitor 3), KIAA0550MGC133100

Entrez Gene IDs

577 (Human); 210933 (Mouse); 301309 (Rat)

Gene Symbol

ADGRB3

UniProt

O60242

Additional BAI3 Products

Product Documents for Human BAI3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BAI3 Antibody

For research use only

Citations for Human BAI3 Antibody

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