Human BATF3 Antibody

Detection of Human BATF3 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BATF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7437) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BATF3 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

BATF3 in HDLM‑2 Human Cell Line.

BATF3 was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Sheep Anti-Human BATF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7437) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of BATF3 by Immunohistochemistry

Genes encoding BATF3 and subunits of the IL-2R are highly acetylated at H3K27 and positively correlate.a Enhancers were ranked based on increasing H3K27ac signals. Genes within SEs in Karpas-299 (ALK+) and Mac-1 (ALK−) ALCL cell lines are marked red. b H3K27ac ChIP-seq tracks at the BATF3 locus with indicated SE regions. c Overlap of genes associated with SEs in Karpas-299 and Mac-1 cell lines. d Enhancers were ranked based on increasing H3K27ac signals. Genes within SEs in primary ALCL patient samples (#54 and #208) are marked in red. e Identification of upstream regulators among deregulated genes in CRISPR/Cas9-mediated BATF3-KO Karpas-299 cells by IPA 2020 analysis. f Analysis of BATF3-correlated genes in a previously published RNA-seq dataset of 23 ALCL patients (BioProject PRJNA255877, SRA identifier SRP044708). Pearson correlation; P value based on t-distribution. g Representative images of 63 patient samples measured of BATF3 and IL-2R alpha IHC staining of ALCL, ALK+ in paired FFPE tissues sections. h IHC quantification of IL-2R alpha, IL-2R beta and IL-2R gamma expression in mature T-NHL TMAs (reactive lymph node controls, n = 11; AITL, n = 8; PTCL-NOS, n = 23; ALCL, ALK+, n = 22; ALCL, ALK−, n = 23) and pcALCL (n = 24) specimens. P values were determined by two-tailed unpaired Student’s t test. All box-whisker plots represent the median (central line), 25th–75th percentile (bounds of the box) and minimum–maximum (whiskers). Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-021-25379-9), licensed under a CC-BY license. Not internally tested by R&D Systems.