Human BLAME/SLAMF8 Biotinylated Antibody BAF1907: R&D Systems

Human BLAME/SLAMF8 Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human BLAME/SLAMF8 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human NTB-A/SLAMF6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human BLAME/SLAMF8
    Ala23-Asp233
    Accession # Q9P0V8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human BLAME/SLAMF8 (Catalog # 1907-BL)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of BLAME/SLAMF8 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was treated for 18 hours with 20 ng/mL Recombinant Human IFN‑ gamma (Catalog # 285-IF) then stained with Human BLAME/SLAMF8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1907, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BLAME/SLAMF8
BLAME, also known as Signaling Lymphocyte Activation Molecule Family Member 8 (SLAMF8), is an Ig superfamily protein that is a member of the SLAM/CD150/CD2 family of receptors. It is expressed on macrophages and dendritic cells. BLAME modulates signaling through the B cell receptor and may also function during B lineage commitment.
  • Entrez Gene IDs:
    56833 (Human)
  • Alternate Names:
    B Lymphocyte Activator Macrophage Expressed; BCM-Like Membrane Protein; BLAME; B-Lymphocyte Activator Macrophage Expressed; CD353 Antigen; CD353; SBBI42; SLAM Family Member 8; SLAMF8
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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