Detection of BLAME/SLAMF8 in U937 Human Cell Line by Flow Cytometry.
U937 human histiocytic lymphoma cell line was treated for 18 hours with 20 ng/mL Recombinant Human IFN‑ gamma (Catalog # 285-IF) then stained with Human BLAME/SLAMF8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1907, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
BLAME, also known as Signaling Lymphocyte Activation Molecule Family Member 8 (SLAMF8), is an Ig superfamily protein that is a member of the SLAM/CD150/CD2 family of receptors. It is expressed on macrophages and dendritic cells. BLAME modulates signaling through the B cell receptor and may also function during B lineage commitment.
Entrez Gene IDs:
56833 (Human); 74748 (Mouse); 289237 (Rat)
B Lymphocyte Activator Macrophage Expressed; BCM-Like Membrane Protein; BLAME; B-Lymphocyte Activator Macrophage Expressed; CD353 Antigen; CD353; SBBI42; SLAM Family Member 8; SLAMF8
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