Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BLAME/SLAMF8 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BLAME/SLAMF8
Ala23-Asp233
Accession # Q9P0V8

Specificity

Detects human BLAME/SLAMF8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human NTB-A/SLAMF6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human BLAME/SLAMF8 Antibody

Detection of Human BLAME/SLAMF8 antibody by Western Blot.

Detection of Human BLAME/SLAMF8 by Western Blot.

Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected, transfected with EGFP, or transfected with human BLAME. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human BLAME/SLAMF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1907) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BLAME/SLAMF8 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of BLAME/SLAMF8 antibody in U937 Human Cell Line antibody by Flow Cytometry.

Detection of BLAME/SLAMF8 in U937 Human Cell Line by Flow Cytometry.

U937 human histiocytic lymphoma cell line were treated for 18 hours with 20 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) then stained with Human BLAME/SLAMF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1907, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Applications for Human BLAME/SLAMF8 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: U937 human histiocytic lymphoma cell line treated with Recombinant Human IFN‑ gamma (Catalog # 285-IF)

Western Blot

0.1 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with human BLAME

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: BLAME/SLAMF8

BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses (1, 2). Mature human BLAME consists of a 211 amino acid (aa) ECD that contains two Ig V-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares 19%‑26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN-gamma stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes (3).

References

  1. McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91.
  2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
  3. Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.

Long Name

SLAM Family Member 8

Alternate Names

CD353, SBBI42, SLAMF8

Entrez Gene IDs

56833 (Human); 74748 (Mouse); 289237 (Rat); 102122568 (Cynomolgus Monkey)

Gene Symbol

SLAMF8

UniProt

Q9P0V8

Additional BLAME/SLAMF8 Products

Product Documents for Human BLAME/SLAMF8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human BLAME/SLAMF8 Antibody

For research use only

Customer Reviews for Human BLAME/SLAMF8 Antibody

There are currently no reviews for this product. Be the first to review Human BLAME/SLAMF8 Antibody and earn rewards!

Have you used Human BLAME/SLAMF8 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies