Human BMP-15/GDF-9B Antibody

Name: Human BMP-15/GDF-9B Antibody
Brand: R&D Systems
SKU: MAB2925-SP
Rating: 5 (1 reviews)

Catalog #: MAB2925 1 Review Datasheet / COA / SDS

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MAB2925
MAB2925-SP

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Human BMP-15/GDF-9B Antibody Summary

Species Reactivity

Human

Specificity

Detects human BMP‑15/GDF‑9B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human BMP-1.1, -2, -3, -3b, -4, -5, -6, -7, -8, or -10 is observed.

Source

Monoclonal Rat IgG_2A Clone # 317809

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human BMP‑15/GDF‑9B
Gln268-Arg392
Accession # O95972

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human BMP‑15/GDF‑9B (Catalog # 5096-BM)

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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BMP‑15/GDF‑9B in Mouse Ovary. BMP‑15/GDF‑9B was detected in immersion fixed frozen sections of mouse ovary using 10 µg/mL Rat Anti-Human BMP‑15/GDF‑9B Monoclonal Antibody (Catalog # MAB2925) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-15/GDF-9B

Bone morphogenetic protein 15 (BMP-15), also known as GDF-9B, is a TGF-beta superfamily ligand that is expressed by oocytes throughout folliculogenesis, and plays an important role in oocyte development (1). BMP-15 promotes the FSH-independent proliferation of ovarian granulosa cells (GC) and induces GC glycolysis and cholesterol synthesis (2‑4). It also induces GC production of stem cell factor which, in turn, negatively regulates BMP-15 expression in oocytes (5). BMP-15 blocks the FSH-induced GC expression of FSH R and multiple steroidogenic molecules (6). BMP-15 is synthesized with a 249 amino acid (aa) N-terminal propeptide (7). The propeptide is cleaved intracellularly from the 50 kDa proBMP-15 but remains associated with mature BMP-15 (8). Mature BMP-15 exists in 16 kDa and 17 kDa forms which are distinguishable by the presence of O-linked glycosylation on the 17 kDa form (8). Mature BMP-15 is phosphorylated, a modification which is required for the stimulation of GC proliferation (9). BMP-15 exerts its effects through interactions with BMPR-IB/ALK6 and BMPR-II (9‑11). Mature BMP-15 forms 34 kDa noncovalently-linked homodimers and 37 kDa heterodimers with mature GDF-9 (12). Both of these oocyte-expressed factors lack the cysteine that mediates disulfide-linked dimerization in most TGF-beta superfamily ligands (1). Although heterodimerization with GDF-9 may limit the secretion of active BMP-15, these two factors synergize in promoting oocyte survival and folliculogenesis (12, 13). Mature human BMP-15 shares 70%, 68%, and 78% aa sequence identity with mouse, rat, and sheep BMP-15, respectively. It shares 27%‑38% aa sequence with other BMPs.

References

Moore, R.K. and S. Shimasaki (2005) Mol. Cell. Endocrinol. 234:67.
Otsuka, F. et al. (2000) J. Biol. Chem. 275:39523.
Sugiura, K. et al. (2007) Development 134:2593.
Su, Y.-Q. et al. (2008) Development 135:111.
Otsuka, F. and S. Shimasaki (2002) Proc. Natl. Acad. Sci. 99:8060.
Otsuka, F. et al. (2001) J. Biol. Chem. 276:11387.
Dube, J.L. et al. (1998) Mol. Endocrinol. 12:1809.
Saito, S. et al. (2008) Prot. Sci. 17:362.
McMahon, H.E. et al. (2008) Endocrinology 149:812.
Moore, R.K. et al. (2003) J. Biol. Chem. 278:304.
Edwards, S.J. et al. (2008) Endocrinology 149:1026.
Liao, W.X. et al. (2003) J. Biol. Chem. 278:3713.
Yan, C. et al. (2001) Mol. Endocrinol. 15:854.

Long Name

Bone Morphogenetic Protein 15

Entrez Gene IDs

9210 (Human); 12155 (Mouse); 59302 (Rat)

Alternate Names

BMP15; BMP-15; bone morphogenetic protein 15; GDF-9B; Growth/differentiation factor 9B; ODG2; POF4

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

This product is sold under license from Vanderbilt University for research and laboratory use only. Licenses for commercial manufacture, commercial use, diagnostics or therapeutics may be obtained by contacting Vanderbilt University, Nashville, Tennessee.