Human BMP-15/GDF-9B Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human BMP‑15/GDF‑9B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human BMP-1.1, -2, -3, -3b, -4, -5, -6, -7, -8, or -10 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 317809
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human BMP‑15/GDF‑9B
    Gln268-Arg392
    Accession # O95972
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human BMP‑15/GDF‑9B (Catalog # 5096-BM)
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
BMP‑15/GDF‑9B in Mouse Ovary. BMP‑15/GDF‑9B was detected in immersion fixed frozen sections of mouse ovary using 10 µg/mL Rat Anti-Human BMP‑15/GDF‑9B Monoclonal Antibody (Catalog # MAB2925) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-15/GDF-9B

Bone morphogenetic protein 15 (BMP-15), also known as GDF-9B, is a TGF-beta superfamily ligand that is expressed by oocytes throughout folliculogenesis, and plays an important role in oocyte development (1). BMP-15 promotes the FSH-independent proliferation of ovarian granulosa cells (GC) and induces GC glycolysis and cholesterol synthesis (2‑4). It also induces GC production of stem cell factor which, in turn, negatively regulates BMP-15 expression in oocytes (5). BMP-15 blocks the FSH-induced GC expression of FSH R and multiple steroidogenic molecules (6). BMP-15 is synthesized with a 249 amino acid (aa) N-terminal propeptide (7). The propeptide is cleaved intracellularly from the 50 kDa proBMP-15 but remains associated with mature BMP-15 (8). Mature BMP-15 exists in 16 kDa and 17 kDa forms which are distinguishable by the presence of O-linked glycosylation on the 17 kDa form (8). Mature BMP-15 is phosphorylated, a modification which is required for the stimulation of GC proliferation (9). BMP-15 exerts its effects through interactions with BMPR-IB/ALK6 and BMPR-II (9‑11). Mature BMP-15 forms 34 kDa noncovalently-linked homodimers and 37 kDa heterodimers with mature GDF-9 (12). Both of these oocyte-expressed factors lack the cysteine that mediates disulfide-linked dimerization in most TGF-beta superfamily ligands (1). Although heterodimerization with GDF-9 may limit the secretion of active BMP-15, these two factors synergize in promoting oocyte survival and folliculogenesis (12, 13). Mature human BMP-15 shares 70%, 68%, and 78% aa sequence identity with mouse, rat, and sheep BMP-15, respectively. It shares 27%‑38% aa sequence with other BMPs.

  • References:
    1. Moore, R.K. and S. Shimasaki (2005) Mol. Cell. Endocrinol. 234:67.
    2. Otsuka, F. et al. (2000) J. Biol. Chem. 275:39523.
    3. Sugiura, K. et al. (2007) Development 134:2593.
    4. Su, Y.-Q. et al. (2008) Development 135:111.
    5. Otsuka, F. and S. Shimasaki (2002) Proc. Natl. Acad. Sci. 99:8060.
    6. Otsuka, F. et al. (2001) J. Biol. Chem. 276:11387.
    7. Dube, J.L. et al. (1998) Mol. Endocrinol. 12:1809.
    8. Saito, S. et al. (2008) Prot. Sci. 17:362.
    9. McMahon, H.E. et al. (2008) Endocrinology 149:812.
    10. Moore, R.K. et al. (2003) J. Biol. Chem. 278:304.
    11. Edwards, S.J. et al. (2008) Endocrinology 149:1026.
    12. Liao, W.X. et al. (2003) J. Biol. Chem. 278:3713.
    13. Yan, C. et al. (2001) Mol. Endocrinol. 15:854.
  • Long Name:
    Bone Morphogenetic Protein 15
  • Entrez Gene IDs:
    9210 (Human); 12155 (Mouse); 59302 (Rat)
  • Alternate Names:
    BMP15; BMP-15; bone morphogenetic protein 15; GDF-9B; GDF9BODG2; Growth/differentiation factor 9B; POF4
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