Detection of Human BMX by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Human BMX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5887) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BMX at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
BMX in Human Prostate. BMX was detected in immersion fixed paraffin-embedded sections of human prostate using Human BMX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5887) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
BMX (Bone marrow tyrosine kinase gene in chromosome X protein; also ETK and NTK38) is a 76‑85 kDa member of the TEC subfamily, Tyr protein kinase family of enzymes. It is a cytoplasmic endothelial and epithelial cell kinase that induces STAT phosphorylation in a Jak-independent manner, and regulates cytokine production via mRNA stabilization. Human BMX is 675 amino acids (aa) in length. It contains one PH domain whose ligation induces activation (aa 6‑109), a zinc-finger region (aa 113‑149), two autophosphorylation sites at Tyr216 and Tyr224, an SH3 and SH2 domain (aa 213‑392) and a kinase catalytic site (aa 417‑675). BMX undergoes proteolysis by caspase 3 to generate a 35 kDa N-terminal (aa 1‑242) fragment, and a 50 kDa C-terminal (aa 243‑675) fragment with enhanced kinase activity.
BMX Non-receptor Tyrosine Kinase
Entrez Gene IDs:
660 (Human); 12169 (Mouse); 367786 (Rat)
BMX non-receptor tyrosine kinase; BMX; Bone marrow tyrosine kinase gene in chromosome X protein; EC 2.7.10; EC 188.8.131.52; Epithelial and endothelial tyrosine kinase; ETK; Etk/Bmx cytosolic tyrosine kinase; ETKcytoplasmic tyrosine-protein kinase BMX; NTK38 tyrosine kinase; NTK38; PSCTK2; PSCTK3
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