|Detection of Human BVES by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BVES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4986) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BVES at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
BVES (blood vessel epicardial substance; also Popeye 1) is a 58 kDa, glycoprotein member of the Popeye family of multispan transmembrane (TM) molecules. It is expressed on embryonic epicardial epithelium plus atrial myocytes and on adult skeletal and cardiac muscle. It serves as a component of the epithelial tight junction, dimerizing with itself and interacting with ZO-1. Human BVES is 360 amino acids (aa) in length. It contains an extracellular N-terminus of 48 aa, three TM domains (aa 49‑113) and a large C-terminal cytoplasmic region (aa 114‑360) that contains a Popeye domain (aa 123‑266). Homodimerization occurs via aa 268‑274. There is one potential splice variant that shows a premature truncation after Glu213. Over aa 1‑36, human BVES is 72% aa identical to mouse BVES.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided