Human BVES Antibody

Catalog #: AF4986 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4986
AF4986-SP

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Human BVES Antibody Summary

Species Reactivity

Human

Specificity

Detects human BVES in direct ELISAs

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BVES
Met1-Asn36
Accession # Q8NE79

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Heart

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of BVES in Human Heart. BVES was detected in immersion fixed paraffin-embedded sections of Human Heart using Sheep Anti-Human BVES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4986) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BVES

BVES (blood vessel epicardial substance; also Popeye 1) is a 58 kDa, glycoprotein member of the Popeye family of multispan transmembrane (TM) molecules. It is expressed on embryonic epicardial epithelium plus atrial myocytes and on adult skeletal and cardiac muscle. It serves as a component of the epithelial tight junction, dimerizing with itself and interacting with ZO-1. Human BVES is 360 amino acids (aa) in length. It contains an extracellular N-terminus of 48 aa, three TM domains (aa 49‑113) and a large C-terminal cytoplasmic region (aa 114‑360) that contains a Popeye domain (aa 123‑266). Homodimerization occurs via aa 268‑274. There is one potential splice variant that shows a premature truncation after Glu213. Over aa 1‑36, human BVES is 72% aa identical to mouse BVES.

Long Name

Blood Vessel Epicardial Substance

Entrez Gene IDs

11149 (Human); 23828 (Mouse)

Alternate Names

blood vessel epicardial substance; BVES; H; HBVES; POP1; POP1MGC42413; POPDC1; POPDC1popeye domain containing 1; Popeye domain-containing protein 1; Popeye protein 1