Human c-Fos Alexa Fluor® 647-conjugated Antibody

Catalog #: AF7254R Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7254R-100UG

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Human c-Fos Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human c-Fos in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human Fos-B is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human c-Fos
Met1-Leu132
Accession # P01100

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: c-Fos

Cellular oncogene fos (c-Fos) is one of four Fos family proteins that associate with Jun family proteins to form the AP-1 transcription factor complex. It is thought to have an important role in signal transduction, cell proliferation and differentiation. Cellular oncogene fos (c-Fos) is constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Within aa 1-132, human c-Fos shares 92% aa sequence identity with mouse and rat c-Fos.

Long Name

FBJ/Finkel–Biskis–Jinkins Murine Osteosarcoma Viral Oncogene Homolog

Entrez Gene IDs

2353 (Human); 14281 (Mouse); 314322 (Rat)

Alternate Names

activator protein 1; AP-1; cellular oncogene c-fos; Cellular oncogene fos; cFos; c-Fos; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; Fos proto-oncogene, AP-1 trancription factor subunit; FOS; G0/G1 switch regulatory protein 7; G0S7; p55; proto-oncogene c-Fos