Human c-Fos Antibody

Catalog # Availability Size / Price Qty
AF7254
AF7254-SP
Detection of Human c-Fos by Western Blot.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human c-Fos Antibody Summary

Species Reactivity
Human
Specificity
Detects human c-Fos in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human Fos-B is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human c-Fos
Met1-Leu132
Accession # P01100
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human c-Fos by Western Blot. View Larger

Detection of Human c-Fos by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 4 hours. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human c-Fos Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7254) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for c-Fos at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry c‑Fos  in HeLa Human Cell Line. View Larger

c‑Fos in HeLa Human Cell Line. c‑Fos was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human c‑Fos Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7254) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: c-Fos

Cellular oncogene fos (c-Fos) is one of four Fos family proteins that associate with Jun family proteins to form the AP-1 transcription factor complex. It is thought to have an important role in signal transduction, cell proliferation and differentiation. Cellular oncogene fos (c-Fos) is constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Within aa 1-132, human c-Fos shares 92% aa sequence identity with mouse and rat c-Fos.

Long Name
FBJ/Finkel–Biskis–Jinkins Murine Osteosarcoma Viral Oncogene Homolog
Entrez Gene IDs
2353 (Human); 14281 (Mouse); 314322 (Rat)
Alternate Names
activator protein 1; AP-1; cellular oncogene c-fos; Cellular oncogene fos; cFos; c-Fos; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; FOS; G0/G1 switch regulatory protein 7; G0S7; proto-oncogene c-Fos; v-fos FBJ murine osteosarcoma viral oncogene homolog

Product Datasheets

Citation for Human c-Fos Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Osteocytes produce interferon-beta as a negative regulator of osteoclastogenesis.
    Authors: Hayashida C, Ito J, Nakayachi M, Okayasu M, Ohyama Y, Hakeda Y, Sato T
    J Biol Chem, 2014;289(16):11545-55.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human c-Fos Antibody

There are currently no reviews for this product. Be the first to review Human c-Fos Antibody and earn rewards!

Have you used Human c-Fos Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review