Human c-Fos Antibody Summary
Accession # P01100
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human c-Fos by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 4 hours. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human c-Fos Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7254) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for c-Fos at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
c‑Fos in HeLa Human Cell Line. c‑Fos was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human c‑Fos Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7254) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cellular oncogene fos (c-Fos) is one of four Fos family proteins that associate with Jun family proteins to form the AP-1 transcription factor complex. It is thought to have an important role in signal transduction, cell proliferation and differentiation. Cellular oncogene fos (c-Fos) is constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Within aa 1-132, human c-Fos shares 92% aa sequence identity with mouse and rat c-Fos.
Citation for Human c-Fos Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Osteocytes produce interferon-beta as a negative regulator of osteoclastogenesis.
Authors: Hayashida C, Ito J, Nakayachi M, Okayasu M, Ohyama Y, Hakeda Y, Sato T
J Biol Chem, 2014;289(16):11545-55.
Sample Types: Cell Lysates
Applications: Western Blot
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