Human c‑Fos Antibody

Name: Human c‑Fos Antibody
Brand: R&D Systems
SKU: AF7254
Price: 159 USD
Availability: InStock

R&D Systems | Catalog # AF7254

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Citations (2)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all c-Fos Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human c-Fos
Met1-Leu132
Accession # P01100

Specificity

Detects human c-Fos in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human Fos-B is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human c‑Fos Antibody

Detection of Human c-Fos by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 4 hours. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human c-Fos Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7254) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for c-Fos at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

c‑Fos in HeLa Human Cell Line.

c-Fos was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human c-Fos Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7254) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human c‑Fos Antibody

Application

Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with PMA

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: c-Fos

Cellular oncogene fos (c-Fos) is one of four Fos family proteins that associate with Jun family proteins to form the AP-1 transcription factor complex. It is thought to have an important role in signal transduction, cell proliferation and differentiation. Cellular oncogene fos (c-Fos) is constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Within aa 1-132, human c-Fos shares 92% aa sequence identity with mouse and rat c-Fos.