|Detection of Human c-Jun by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MDA‑MB‑468 human breast cancer cell lines. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human c-Jun Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2670) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for c-Jun at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
c-Jun is one of three Jun family proteins making up the Activator Protein-1 (AP-1) transcription factor group. c-Jun acts as a positive regulator of cell proliferation and can induce oncogenic transformation.
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