Human c-Jun Antibody

Catalog # Availability Size / Price Qty
AF2670
AF2670-SP
Detection of Human c-Jun by Western Blot.
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Product Details
Citations (1)
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Human c-Jun Antibody Summary

Species Reactivity
Human
Specificity
Detects human c-Jun in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human c-Jun
Thr2-Phe331
Accession # P05412
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human c-Jun antibody by Western Blot. View Larger

Detection of Human c-Jun by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MDA-MB-468 human breast cancer cell lines. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human c-Jun Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2670) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for c-Jun at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: c-Jun

c-Jun is one of three Jun family proteins making up the Activator Protein-1 (AP-1) transcription factor group. c-Jun acts as a positive regulator of cell proliferation and can induce oncogenic transformation.

Long Name
Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1
Entrez Gene IDs
3725 (Human)
Alternate Names
Activator protein 1; AP1; AP-1; cJun; c-Jun; enhancer-binding protein AP1; Jun activation domain binding protein; jun oncogene; jun proto-oncogene; JUN; Proto-oncogene c-Jun; transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homologp39; v-jun sarcoma virus 17 oncogene homolog (avian); v-jun sarcoma virus 17 oncogene homolog

Product Datasheets

Citation for Human c-Jun Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Characterization of short range DNA looping in endotoxin-mediated transcription of the murine inducible nitric-oxide synthase (iNOS) gene.
    Authors: Guo H, Mi Z, Kuo PC
    J. Biol. Chem., 2008;283(37):25209-17.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP

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