Human C-Reactive Protein/CRP Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1707
Product Details
FAQs
Supplemental Products
Reviews

Human C-Reactive Protein/CRP Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects C‑Reactive Protein/CRP in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse CRP is observed and 1% cross-reactivity with recombinant rat CRP is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human C‑Reactive Protein/CRP
Phe17-Pro224
Accession # P02741
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human C-Reactive Protein/CRP (Catalog # 1707-CR)
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human renal cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C-Reactive Protein/CRP

CRP is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are non-covalently linked to form the pentameter. Human CRP shares 71% and 64% amino acid sequence homology with mouse and rat respectively.

CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL-6, IL-1 and glucocorticoids are the major inducer of the CRP gene. In response to infection, inflammation or tissue damage, the level of CRP in human serum can increase 1,000-fold within 24‑48 hours. It will come back to base level of less than 1 μg/mL very fast. Human CRP is an acute-phase serum protein that plays a role in the first line in host innate host defense. Like other pentraxins, CRP exhibits Ca++ - dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal walls, is a principal ligand of CRP. CRP also binds to the membrane of injured cells, membrane and nuclear components of necrotic and apoptotic cells. Upon binding with the ligands, CRP is recognized by C1q and initiates the activation of complement cascade. Ligand bound CRP also binds to Fc gamma RI and Fc gamma RIIa on phagocytes and activates phogocytotic responses. In addition to phogocytosis, CRP also can induce production of hydrogen peroxide and inflammatory cytokines, such as IL-1, IL-6 and TNF‑ alpha  by monocytes. With these functions, human CRP is an important serum protein for anti-bacterial pathogen and clearance of damaged and apoptotic cells. However, in mouse, CRP is expressed at very low level and is not an acute phase reactant. Serum amyloid P component (SAP), another pentraxin, is the major acute phase serum protein in mice. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.

References
  1. Gotschlich, E.C. and G.M. Edelman (1965) Proc. Natl. Acad. Sci. USA 54:558.
  2. Volanakis, J.E. (2001) Molecular Immunology 38:189.
  3. Bharadwaj, D. et al. (1999) J. Experimental Medicine 190:585.
  4. Ballou S.P. and G. Lozanski (1992) Cytokine 4:361.
  5. Danesh, J. et al. (2004) N. Engl. J. Med. 350:1387.
Entrez Gene IDs
1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Staining Reagents

Supplemental Cell Selection Products

Supplemental ELISA Products

Reviews for Human C-Reactive Protein/CRP Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human C-Reactive Protein/CRP Biotinylated Antibody and earn rewards!

Have you used Human C-Reactive Protein/CRP Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review