Human Calcium Activated Nucleotidase 1/CANT1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Calcium Activated Nucleotidease 1/CANT1 in direct ELISAs.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Calcium Activated Nucleotidease 1/CANT1
    Gly80-Ile401
    Accession # Q8WVQ1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Calcium Activated Nucleotidase 1/CANT1 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Calcium Activated Nucleotidase 1/CANT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6720) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Calcium Activated Nucleotidase 1/CANT1 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Calcium Activated Nucleotidase 1/CANT1 in PC‑3 Human Cell Line.
Calcium Activated Nucleotidase 1/CANT1 was detected in immersion fixed PC‑3 human prostate cancer cell line using Sheep Anti-Human Calcium Activated Nucleotidase 1/
CANT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6720) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL011) and counterstained with DAPI (blue). Specific staining was localized to perinuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Calcium Activated Nucleotidase 1/CANT1

CANT1 (soluble Calcium-Activated NucleoTidase 1; also SCAN-1, Apyrase homolog and NF kappa B-activating protein 107) is a 45-48 kDa glycoprotein member of the apyrase (ADP/ATP hydrolysing) family of molecules. It is an ER and Golgi-embedded protein that hydrolyzes UDP in a calcium-dependent manner, and appears to be involved in the regulation of protein glycosylation and folding. It is also reported that CANT1 is essential for cell proliferation. CANT1 is not ubiquitous, but is found in multiple cell types, including prostatic epithelium, fibroblasts, lymphocytes and chondrocytes. Human CANT1 is a 401 amino acid (aa) type II transmembrane glycoprotein. It contains a 44 aa N-terminal cytoplasmic region that possesses an ER retention motif (aa 38-42), and a 339 aa luminal domain (aa 63-401). CANT1 is reported to form membrane-bound disulfide-linked homodimers, and potentially form non-covalent homodimers in a soluble (circulating) state. The soluble form is 35‑40 kDa in size, and presumably cleaved near the transmembrane segment. However, multiple splice forms are possible, and may account for the lower MWs. Two splice forms impact the extracellular region, one which contains a four aa substitution for aa 219-401, and a second that possesses a 26 aa substitution for aa 220‑401. Two additional splice forms utilize alternative start sites at Met 31 and Met165. Over aa 80-401, human CANT1 shares 89% aa sequence identity with mouse CANT1.

  • Long Name:
    Soluble Calcium Activated Nucleotidase 1
  • Entrez Gene IDs:
    124583 (Human); 76025 (Mouse); 246272 (Rat)
  • Alternate Names:
    Apyrase homolog; calcium activated nucleotidase 1; CANT1; DBQD; EC 3.6.1.6; Putative MAPK-activating protein PM09; Putative NF-kappa-B-activating protein 107; SCAN1; SCAN-1Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase; SHAPY; SHAPYmicromelic dwarfism with vertebral and metaphyseal abnormalities and advancedcarpotarsal ossification; soluble Ca-activated nucleotidase, isozyme 1; soluble calcium-activated nucleotidase 1; soluble calcium-activated nucleotidase SCAN-1
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