|Calsyntenin‑2 in Human Brain. Calsyntenin‑2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human Calsyntenin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5480) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
Calsyntenin-2 (CS-2) is a 120-135 kDa member of the Alcadein family of molecules. It is expressed in the ER/Golgi, and the plasma membranes of almost all neurons. It is a calcium-binding protein that interacts with APP and X11L. In this regard, it appears to regulate APP cleavage and gene activation, and likely impacts post-synaptic signaling. Notably, when cleaved in a manner similar to APP, its intracellular fragment antagonizes AICD gene activation. Mature human CS-2 is a 935 amino acid (aa) type I transmembrane protein. It contains two cadherin domains (aa 44-280) in its extracellular region (aa 21-831) and a 103 aa cytoplasmic tail. Proteolytic cleavage by ADAM10 occurs between His803 and Leu804 to generate a 105-110 kDa extracellular fragment. Additional processing of the transmembrane fragment by gamma -secretase creates a 3 kDa fragment (aa 804-834) plus a 25 kDa cytoplasmic protein. Potential isoform variants show a premature truncation after Gln608, and the use of an alternative start site at Met409. Over aa 21-834, human CS-2 shares 95% aa identity with mouse CS-2.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided