Human Calsyntenin-2 Antibody Summary
Accession # Q9H4D0
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Calsyntenin‑2 in Human Brain. Calsyntenin‑2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human Calsyntenin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5480) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calsyntenin-2 (CS-2) is a 120-135 kDa member of the Alcadein family of molecules. It is expressed in the ER/Golgi, and the plasma membranes of almost all neurons. It is a calcium-binding protein that interacts with APP and X11L. In this regard, it appears to regulate APP cleavage and gene activation, and likely impacts post-synaptic signaling. Notably, when cleaved in a manner similar to APP, its intracellular fragment antagonizes AICD gene activation. Mature human CS-2 is a 935 amino acid (aa) type I transmembrane protein. It contains two cadherin domains (aa 44-280) in its extracellular region (aa 21-831) and a 103 aa cytoplasmic tail. Proteolytic cleavage by ADAM10 occurs between His803 and Leu804 to generate a 105-110 kDa extracellular fragment. Additional processing of the transmembrane fragment by gamma -secretase creates a 3 kDa fragment (aa 804-834) plus a 25 kDa cytoplasmic protein. Potential isoform variants show a premature truncation after Gln608, and the use of an alternative start site at Met409. Over aa 21-834, human CS-2 shares 95% aa identity with mouse CS-2.
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Cell and Tissue Staining Kits
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