Detection of Human CARF by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, SK-Mel-28 human malignant melanoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Human CARF Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4195) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CARF at approximately 61 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CARF (collaborator of ARF) is a component of the ARF-MDM2-p53 pathway. CARF interacts with ARF in the nucleolus accentuating ARF-mediated p53 stabilization and activation. Data also suggests that in the absence of ARF, CARF interacts directly with p53 in the nucleoplasm resulting in a moderate stabilization and activation of p53.
Collaborates/Cooperates With ARF [Alternate Reading Frame] Protein
Entrez Gene IDs:
55602 (Human); 70925 (Mouse)
CARF; CARFCDKN2A-interacting protein; CDKN2A interacting protein; CDKN2AIP; collaborates/cooperates with ARF (alternate reading frame) protein; Collaborator of ARF; FLJ20036
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