Human Caspr2 Antibody

Catalog # Availability Size / Price Qty
MAB5145-100
MAB5145-SP
Human Caspr2 Antibody in Flow Cytometry
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Human Caspr2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Caspr2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 840727
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Caspr2
Pro29-Ala1262
Accession # Q9UHC6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Caspr2 in HEK293 Human Cell Line Transfected with Human Caspr2 and eGFP by Flow Cytometry. View Larger

Detection of Caspr2 in HEK293 Human Cell Line Transfected with Human Caspr2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Caspr2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Caspr2 Monoclonal Antibody (Catalog # MAB5145) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspr2

Caspr2 (CNTNAP2, Contactin-associated protein-like 2 and Cell recognition molecule Caspr2) is a 160-170 kDa glycoprotein, a member of the neurexin family of proteins.  It is predominantly expressed in nervous system and likely play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction. Caspr2 and closely related molecule Caspr/Paranodin demarcate distinct subdomains in myelinated axons. While contactin-associated protein (Caspr) is present at the paranodal junctions, Caspr2 is precisely colocalized with Shaker-like K+ channels in the juxtaparanodal region.  Over aa 29-1262, human CASPR2 shares 94% aa sequence identity with mouse Caspr2.                    

Long Name
Contactin Associated Protein-like 2
Entrez Gene IDs
26047 (Human); 66797 (Mouse)
Alternate Names
AUTS15; Caspr2; CASPR2DKFZp781D1846; CDFE; Cell recognition molecule Caspr2; CNTNAP2; contactin associated protein-like 2; contactin-associated protein 2; contactin-associated protein-like 2; homolog of Drosophila neurexin IV; KIAA0868; NRXN4; NRXN4Caspr2; PTHSL1

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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