Human Cathepsin S Antibody [Biotin]

R&D Systems | Catalog # BAF1183

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Cathepsin S Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cathepsin S
Gln17-Ile331
Accession # P25774

Specificity

Detects human Cathepsin S in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human Cathepsin A, -B, -C, -D, E, L, V, and -X/Z/P is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Cathepsin S Antibody [Biotin]

Cathepsin S antibody in Human Lymph Node by Immunohistochemistry (IHC-P).

Cathepsin S in Human Lymph Node.

Cathepsin S was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human Cathepsin S Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1183) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Human Total Cathepsin S ELISA Standard Curve

Human Total Cathepsin S ELISA Standard Curve

Recombinant Human Cathepsin S (Catalog # 1183-CY) was serially diluted and captured by Goat Anti-Human Cathepsin S Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1183) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Cathepsin S Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1183) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Cathepsin S Antibody [Biotin]

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node

Western Blot

0.1 µg/mL
Sample: Recombinant Human Cathepsin S (Catalog # 1183-CY)

Human Cathepsin S Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human Cathepsin S Antibody (Catalog # AF1183)
  • Standard: Recombinant Human Cathepsin S Protein, CF (Catalog # 1183-CY)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cathepsin S

Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1‑16), a pro region (residues 17‑114), and the mature enzyme (residues 115‑331) (3‑5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages and other phagocytic cells.

References

  1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104, Academic Press, San Diego.
  2. Turk, V. et al. (2001) EMBO J. 20:4629.
  3. Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
  4. Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
  5. Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.

Alternate Names

CTSS

Entrez Gene IDs

1520 (Human)

Gene Symbol

CTSS

UniProt

P25774

Additional Cathepsin S Products

Product Documents for Human Cathepsin S Antibody [Biotin]

Certificate of Analysis

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Product Specific Notices for Human Cathepsin S Antibody [Biotin]

For research use only

Citations for Human Cathepsin S Antibody [Biotin]

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Protocols

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