Human Cathepsin S Antibody

Catalog # Availability Size / Price Qty
AF1183
AF1183-SP
Human Cathepsin S Antibody in Immunohistochemistry
1 Image
Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (1)

Human Cathepsin S Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cathepsin S in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human Cathepsin A, B, C, D, E, L, V, and X/Z/P is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin S
Gln17-Ile331
Accession # P25774
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Cathepsin S (Catalog # 1183-CY)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Cathepsin S (Catalog # 1183-CY), see our available Western blot detection antibodies

Human Cathepsin S Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human Cathepsin S Biotinylated Antibody (Catalog # BAF1183)

Standard: Recombinant Human Cathepsin S Protein, CF (Catalog # 1183-CY)

Neutralization
Measured by its ability to neutralize activation and the resulting activity of Recombinant Human Cathepsin S (0.25 µg/mL, Catalog # 1183-CY) in cleaving the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 0.2-1.8 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Cathepsin S in Human Lymph Node. View Larger

Cathepsin S in Human Lymph Node. Cathepsin S was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human Cathepsin S Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1183) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin S

Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1-16), a pro region (residues 17-114), and the mature enzyme (residues 115-331) (3-5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages, and other phagocytic cells.

References
  1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104 - 1107, Academic Press, San Diego.
  2. Turk, V. et al. (2001) EMBO J. 20:4629.
  3. Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
  4. Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
  5. Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.
Entrez Gene IDs
1520 (Human)
Alternate Names
Cathepsin S; CTSS; EC 3.4.22; EC 3.4.22.27; FLJ50259; MGC3886

Product Datasheets

Citations for Human Cathepsin S Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases
    Authors: Y Hamon, M Legowska, V Hervé, S Dallet-Cho, S Marchand-A, L Vanderlynd, M Demonte, R Williams, CJ Scott, M Si-Tahar, N Heuzé-Vour, G Lalmanach, DE Jenne, A Lesner, F Gauthier, B Korkmaz
    J. Biol. Chem., 2016;291(16):8486-99.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors
    Sci Rep, 2016;6(0):29256.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. The Unusual Resistance of Avian Defensin AvBD7 to Proteolytic Enzymes Preserves Its Antibacterial Activity
    PLoS ONE, 2016;11(8):e0161573.
    Species: Chicken
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Evaluating the diagnostic and prognostic value of circulating cathepsin S in gastric cancer
    Oncotarget, 2016;7(19):28124-38.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer.
    Authors: Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K
    PLoS ONE, 2015;10(4):e0120348.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Cathepsin S cannibalism of cathepsin K as a mechanism to reduce type I collagen degradation.
    Authors: Barry, Zachary, Platt, Manu O
    J Biol Chem, 2012;287(33):27723-30.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  7. Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
    Authors: Shree T, Olson OC, Elie BT
    Genes Dev., 2011;25(23):2465-79.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Antibody targeting of Cathepsin S induces antibody-dependent cellular cytotoxicity.
    Authors: Kwok HF, Buick RJ, Kuehn D
    Mol. Cancer, 2011;10(0):147.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Human cysteine cathepsins are not reliable markers of infection by Pseudomonas aeruginosa in cystic fibrosis.
    Authors: Naudin C, Joulin-Giet A, Couetdic G, Plesiat P, Szymanska A, Gorna E, Gauthier F, Kasprzykowski F, Lecaille F, Lalmanach G
    PLoS ONE, 2011;6(9):e25577.
    Species: Human
    Sample Types: Sputum
    Applications: Western Blot
  10. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  11. Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
    Authors: Wendt W, Schulten R, Stichel CC, Lubbert H
    J. Neurochem., 2009;110(6):1931-41.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Upregulation of cathepsin S in the aging and pathological nervous system of mice.
    Authors: Wendt W, Lubbert H, Stichel CC
    Brain Res., 2008;1232(0):7-20.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Distinct roles for cysteine cathepsin genes in multistage tumorigenesis.
    Authors: Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA
    Genes Dev., 2006;20(5):543-56.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

ExactaChIP Chromatin Immunoprecipitation Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Cathepsin S Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Cathepsin S Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Cathepsin S Antibody
By Anonymous on 12/01/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human