Human Cathepsin S Antibody
Human Cathepsin S Antibody Summary
Gln17-Ile331
Accession # P25774
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Applications
Human Cathepsin S Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
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Cathepsin S in Human Lymph Node. Cathepsin S was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human Cathepsin S Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1183) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin S
Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1-16), a pro region (residues 17-114), and the mature enzyme (residues 115-331) (3-5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages, and other phagocytic cells.
- Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104 - 1107, Academic Press, San Diego.
- Turk, V. et al. (2001) EMBO J. 20:4629.
- Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
- Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
- Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.
Product Datasheets
Citations for Human Cathepsin S Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Cathepsin S Alterations Induce a Tumor-Promoting Immune Microenvironment in Follicular Lymphoma
Authors: D Bararia, JA Hildebrand, S Stolz, S Haebe, S Alig, CP Trevisani, F Osorio-Bar, MD Bartoschek, M Mentz, A Pastore, E Gaitzsch, M Heide, V Jurinovic, K Rautter, J Gunawardan, MB Sabdia, M Szczepanow, J Richter, W Klapper, A Louissaint, C Ludwig, S Bultmann, H Leonhardt, S Eustermann, KP Hopfner, W Hiddemann, M von Bergwe, C Steidl, R Kridel, JWD Tobin, MK Gandhi, DM Weinstock, M Schmidt-Su, MB Sárosi, M Rudelius, V Passerini, J Mautner, O Weigert
Cell Rep, 2020;31(5):107522.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases
Authors: Y Hamon, M Legowska, V Hervé, S Dallet-Cho, S Marchand-A, L Vanderlynd, M Demonte, R Williams, CJ Scott, M Si-Tahar, N Heuzé-Vour, G Lalmanach, DE Jenne, A Lesner, F Gauthier, B Korkmaz
J. Biol. Chem., 2016;291(16):8486-99.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors
Sci Rep, 2016;6(0):29256.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The Unusual Resistance of Avian Defensin AvBD7 to Proteolytic Enzymes Preserves Its Antibacterial Activity
PLoS ONE, 2016;11(8):e0161573.
Species: Chicken
Sample Types: Tissue Homogenates
Applications: Western Blot -
Evaluating the diagnostic and prognostic value of circulating cathepsin S in gastric cancer
Oncotarget, 2016;7(19):28124-38.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer.
Authors: Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K
PLoS ONE, 2015;10(4):e0120348.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Cathepsin S cannibalism of cathepsin K as a mechanism to reduce type I collagen degradation.
Authors: Barry, Zachary, Platt, Manu O
J Biol Chem, 2012;287(33):27723-30.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
Authors: Shree T, Olson OC, Elie BT
Genes Dev., 2011;25(23):2465-79.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Antibody targeting of Cathepsin S induces antibody-dependent cellular cytotoxicity.
Authors: Kwok HF, Buick RJ, Kuehn D
Mol. Cancer, 2011;10(0):147.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human cysteine cathepsins are not reliable markers of infection by Pseudomonas aeruginosa in cystic fibrosis.
Authors: Naudin C, Joulin-Giet A, Couetdic G, Plesiat P, Szymanska A, Gorna E, Gauthier F, Kasprzykowski F, Lecaille F, Lalmanach G
PLoS ONE, 2011;6(9):e25577.
Species: Human
Sample Types: Sputum
Applications: Western Blot -
A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010;56(0):1320.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
Authors: Wendt W, Schulten R, Stichel CC, Lubbert H
J. Neurochem., 2009;110(6):1931-41.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Upregulation of cathepsin S in the aging and pathological nervous system of mice.
Authors: Wendt W, Lubbert H, Stichel CC
Brain Res., 2008;1232(0):7-20.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Distinct roles for cysteine cathepsin genes in multistage tumorigenesis.
Authors: Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA
Genes Dev., 2006;20(5):543-56.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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