Human Cathepsin S Antibody

• Species Reactivity
  Human
• Specificity
  Detects human Cathepsin S in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human Cathepsin A, B, C, D, E, L, V, and X/Z/P is observed.
• Source
  Polyclonal Goat IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  Mouse myeloma cell line NS0-derived recombinant human Cathepsin S
  Gln17-Ile331
  Accession # P25774
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  0.1 µg/mL
  Recombinant Human Cathepsin S (Catalog # 1183-CY)
• Immunohistochemistry
  5-15 µg/mL
  See below
• Immunoprecipitation
  25 µg/mL
  Conditioned cell culture medium spiked with Recombinant Human Cathepsin S (Catalog # 1183-CY), see our available Western blot detection antibodies
• Human Cathepsin S Sandwich Immunoassay
  Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 
Human Cathepsin S Antibody (Catalog # AF1183 )
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 
Human Cathepsin S Biotinylated Antibody (Catalog # BAF1183 )
ELISA Standard
 
Recombinant Human Cathepsin S Protein, CF (Catalog # 1183-CY )
Neutralization
Measured by its ability to neutralize activation and the resulting activity of Recombinant Human Cathepsin S (0.25 µg/mL, Catalog # 1183-CY) in cleaving the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (10 µM, Catalog # ES002). The Neutralization Dose (ND₅₀) is typically 2 µg/mL.

Immunohistochemistry click image to view larger

Cathepsin S in Human Lymph Node. Cathepsin S was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human Cathepsin S Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1183) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

• Reconstitution
  Reconstitute at 0.2 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104 - 1107, Academic Press, San Diego.
  2. Turk, V. et al. (2001) EMBO J. 20:4629.
  3. Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
  4. Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
  5. Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.

• Entrez Gene IDs:
  1520 (Human)

• Alternate Names:
  Cathepsin S; CTSS; EC 3.4.22; EC 3.4.22.27; FLJ50259; MGC3886

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