Human Cathepsin S Antibody

(13 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Cathepsin S in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human Cathepsin A, B, C, D, E, L, V, and X/Z/P is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Cathepsin S
    Gln17-Ile331
    Accession # P25774
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Cathepsin S (Catalog # 1183-CY)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human Cathepsin S (Catalog # 1183-CY), see our available Western blot detection antibodies
    • Human Cathepsin S Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human Cathepsin S Antibody (Catalog # AF1183)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human Cathepsin S Biotinylated Antibody (Catalog # BAF1183)
  • ELISA Standard
     
    Recombinant Human Cathepsin S Protein, CF (Catalog # 1183-CY)
  • Neutralization
    Measured by its ability to neutralize activation and the resulting activity of Recombinant Human Cathepsin S (0.25 µg/mL, Catalog # 1183-CY) in cleaving the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 2 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Cathepsin S in Human Lymph Node. Cathepsin S was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human Cathepsin S Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1183) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin S

Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1-16), a pro region (residues 17-114), and the mature enzyme (residues 115-331) (3-5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages, and other phagocytic cells.

  • References:
    1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104 - 1107, Academic Press, San Diego.
    2. Turk, V. et al. (2001) EMBO J. 20:4629.
    3. Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
    4. Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
    5. Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.
  • Entrez Gene IDs:
    1520 (Human)
  • Alternate Names:
    Cathepsin S; CTSS; EC 3.4.22; EC 3.4.22.27; FLJ50259; MGC3886
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. The Unusual Resistance of Avian Defensin AvBD7 to Proteolytic Enzymes Preserves Its Antibacterial Activity
    PLoS ONE, 2016;11(8):e0161573.
    Species: Chicken
    Sample Type: Tissue Homogenates
    Application: WB
  2. Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors
    Sci Rep, 2016;6(0):29256.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Evaluating the diagnostic and prognostic value of circulating cathepsin S in gastric cancer
    Oncotarget, 2016;7(19):28124-38.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases
    Authors: Y Hamon, M Legowska, V Hervé, S Dallet-Cho, S Marchand-A, L Vanderlynd, M Demonte, R Williams, CJ Scott, M Si-Tahar, N Heuzé-Vour, G Lalmanach, DE Jenne, A Lesner, F Gauthier, B Korkmaz
    J. Biol. Chem., 2016;291(16):8486-99.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer.
    Authors: Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K
    PLoS ONE, 2015;10(4):e0120348.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  6. Cathepsin S cannibalism of cathepsin K as a mechanism to reduce type I collagen degradation.
    Authors: Barry, Zachary, Platt, Manu O
    J Biol Chem, 2012;287(33):27723-30.
    Species: Human
    Sample Type: Protein
    Application: WB
  7. Antibody targeting of Cathepsin S induces antibody-dependent cellular cytotoxicity.
    Authors: Kwok HF, Buick RJ, Kuehn D
    Mol. Cancer, 2011;10(0):147.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
    Authors: Shree T, Olson OC, Elie BT
    Genes Dev., 2011;25(23):2465-79.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Human cysteine cathepsins are not reliable markers of infection by Pseudomonas aeruginosa in cystic fibrosis.
    Authors: Naudin C, Joulin-Giet A, Couetdic G, Plesiat P, Szymanska A, Gorna E, Gauthier F, Kasprzykowski F, Lecaille F, Lalmanach G
    PLoS ONE, 2011;6(9):e25577.
    Species: Human
    Sample Type: Sputum
    Application: WB
  10. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  11. Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
    Authors: Wendt W, Schulten R, Stichel CC, Lubbert H
    J. Neurochem., 2009;110(6):1931-41.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  12. Upregulation of cathepsin S in the aging and pathological nervous system of mice.
    Authors: Wendt W, Lubbert H, Stichel CC
    Brain Res., 2008;1232(0):7-20.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  13. Distinct roles for cysteine cathepsin genes in multistage tumorigenesis.
    Authors: Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA
    Genes Dev., 2006;20(5):543-56.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Expand to show all 13 Citations
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ApplicationELISA
Sample TestedEDTA Plasma
SpeciesHuman

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