Human CCL1/I‑309/TCA‑3 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF272
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human CCL1/I‑309/TCA‑3 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL1/I‑309/TCA‑3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with rhMIP-1 beta, rvMIP-1, rrIFN-gamma, rmTCA-3 and rhTARC is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL1/I‑309/TCA‑3 (R&D Systems, Catalog # 272-I)
Lys24-Lys96
Accession # P22362
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL1/I‑309/TCA‑3 (Catalog # 272-I)

Human CCL1/I-309 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CCL1/I‑309/TCA‑3 Antibody (Catalog # MAB272)

Standard: Recombinant Human CCL1/I-309 Protein (Catalog # 272-I)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL1/I-309/TCA-3

Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes.

CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The CCL1 sequence contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15-16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.

References
  1. Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci. USA 89:2950.
  2. Miller, M.D. et al. (1990) J. Immunol. 145:2737.
Entrez Gene IDs
6346 (Human); 20290 (Mouse)
Alternate Names
C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3

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Citations for Human CCL1/I‑309/TCA‑3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CC chemokine ligand 1 is released into the airways of atopic asthmatics.
    Authors: Montes-Vizuet R, Vega-Miranda A, Valencia-Maqueda E, Negrete-Garcia MC, Velasquez JR, Teran LM
    Eur. Respir. J., 2006;28(1):59-67.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development
  2. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
    Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
    J. Leukoc. Biol., 2001;70(4):617-23.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Reviews for Human CCL1/I‑309/TCA‑3 Biotinylated Antibody

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Human CCL1/I‑309/TCA‑3 Biotinylated Antibody
By Anonymous on 11/22/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

MAB272 was used as the capture and BAF272 as the detection in a sandwich immunoassay. The immunoassay standard was 272-I. It worked very well. Most healthy human samples were not detectable.