Human CCL1/I-309/TCA-3 Antibody Summary
Accession # P22362
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCL1/I‑309 Antibody. Recombinant Human CCL1/I‑309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I‑309 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL1/I‑309 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF272). The ND50 is typically 0.4-2.0 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV‑transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes.
CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The CCL1 sequence contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15‑16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.
- Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci USA 89:2950.
- Miller, M.D. et al. (1990) J. Immunol. 145:2737.
Citations for Human CCL1/I-309/TCA-3 Antibody
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Comprehensive assessment of chemokine expression profiles by flow cytometry.
Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
J. Clin. Invest., 2010;120(3):907-23.
Sample Types: Whole Cells
Applications: Flow Cytometry
CC chemokine ligand 1 is released into the airways of atopic asthmatics.
Authors: Montes-Vizuet R, Vega-Miranda A, Valencia-Maqueda E, Negrete-Garcia MC, Velasquez JR, Teran LM
Eur. Respir. J., 2006;28(1):59-67.
Sample Types: BALF
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