Human CCL20/MIP‑3 alpha Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF360

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CCL20/MIP‑3 alpha  in Human Lymph Node.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Human CCL20/MIP‑3 alpha Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL20/MIP-3 alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human (rh) MIP‑3 beta, recombinant mouse (rm) MIP‑3 beta, rhMCP-1, rmMIP‑3 alpha, recombinant rat MIP‑3 alpha, and rmJE/MCP-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL20/MIP-3 alpha
Ala27-Met96 (Asn95Asp)
Accession # P78556
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL20/MIP‑3 alpha (Catalog # 360-MP)
Immunohistochemistry
5-15 µg/mL
See below

Human CCL20/MIP-3 alpha Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CCL20/MIP‑3 alpha Antibody (Catalog # MAB360)

Standard: Recombinant Human CCL20/MIP-3 alpha Protein (Catalog # 360-MP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry CCL20/MIP‑3 alpha  in Human Lymph Node. View Larger

CCL20/MIP‑3 alpha in Human Lymph Node. CCL20/MIP‑3a was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Goat Anti-Human CCL20/MIP‑3a Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF360) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL20/MIP-3 alpha

CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17.

CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

References
  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.
Entrez Gene IDs
6364 (Human); 20297 (Mouse); 29538 (Rat)
Alternate Names
beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38

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Citations for Human CCL20/MIP‑3 alpha Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. CCL20 secreted from IgA1-stimulated human mesangial cells recruits inflammatory Th17 cells in IgA nephropathy
    Authors: G Lu, X Zhang, L Shen, Q Qiao, Y Li, J Sun, J Zhang
    PLoS ONE, 2017;12(5):e0178352.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  3. Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients.
    Authors: Lecureuil C, Combadiere B, Mazoyer E, Bonduelle O, Samri A, Autran B, Debre P, Combadiere C
    Blood, 2007;109(9):3649-57.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  4. Paired synovium and lymph nodes from rheumatoid arthritis patients differ in dendritic cell and chemokine expression.
    Authors: Page G, Miossec P
    J. Pathol., 2004;204(1):28-38.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Reviews for Human CCL20/MIP‑3 alpha Biotinylated Antibody

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Human CCL20/MIP‑3 alpha Biotinylated Antibody
By Anonymous on 12/07/2017
Application: ELISA Sample Tested: Serum Species: Human

Good detection in disease samples