Human CD14 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DC140
Control Products Available
Human CD14 Standard Curve
2 Images
Product Details
Procedure
Citations (67)
FAQs
Supplemental Products
Reviews (5)

Human CD14 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
Sensitivity
125 pg/mL
Assay Range
250.0 - 16,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human sCD14
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human soluble CD14 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human soluble CD14 in cell culture supernates, serum, and plasma. It contains recombinant human CD14 expressed from CHO cells and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human sCD14 showed dose-response curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human sCD14.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 1111 2158 4187 1159 2298 4346
Standard Deviation 71 104 216 86 153 209
CV% 6.4 4.8 5.2 7.4 6.7 4.8

Recovery

The recovery of sCD14 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 91 85-105
Citrate Plasma (n=5) 102 88-110
EDTA Plasma (n=5) 98 90-107
Heparin Plasma (n=5) 96 91-104
Serum (n=5) 98 84-107

Linearity

To assess the linearity of the assay, five samples containing or spiked with high concentrations of sCD14 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CD14 ELISA Linearity

Data Examples

Human CD14 Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD14

CD14 is an acute phase glycoprotein that binds lipopolysaccharide endotoxins (LPS) from Gram-negative bacteria. It is primarily expressed on monocytes, macrophages, and neutrophils, and soluble forms can be secreted or shed from the cell surface. CD14 accepts LPS from its initial binding by LBP, and it can further transfer LPS to the TLR4/MD2 complex. LPS acctivation of TLR4 triggers inflammatory activation. CD14 also contributes to the response to TLR3 ligands, phosphoinositides, as well as the surfactant proteins SP-A and SP-D.

Entrez Gene IDs:
929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
Alternate Names:
CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human CD14 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

67 Citations: Showing 1 - 10
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  1. HIV-1 proviral landscapes distinguish posttreatment controllers from noncontrollers
    Authors: R Sharaf, GQ Lee, X Sun, B Etemad, LM Aboukhater, Z Hu, ZL Brumme, E Aga, RJ Bosch, Y Wen, G Namazi, C Gao, EP Acosta, RT Gandhi, JM Jacobson, D Skiest, DM Margolis, R Mitsuyasu, P Volberding, E Connick, DR Kuritzkes, MM Lederman, XG Yu, M Lichterfel, JZ Li
    J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Plasma
  2. The CD14 (-159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes
    Authors: JJ Nieto-Font, FJ Salgado, ME San-José, MJ Cruz, A Casas-Fern, MJ Gómez-Cond, L Valdés-Cua, MÁ García-Gon, P Arias, M Nogueira, FJ González-B
    Sci Rep, 2018;8(1):4147.
    Species: Human
    Sample Types: Serum
  3. Fecal Bacterial Communities in treated HIV infected individuals on two antiretroviral regimens
    Authors: S Pinto-Card, C Lozupone, O Briceño, S Alva-Herná, N Téllez, A Adriana, A Murakami-O, G Reyes-Terá
    Sci Rep, 2017;7(0):43741.
    Species: Human
    Sample Types: Plasma
  4. Interleukin-1 receptor antagonist, a biomarker of response to anti-TB treatment in HIV/TB co-infected patients
    Authors: J Nouhin, P Pean, Y Madec, MF Chevalier, C Didier, L Borand, FX Blanc, D Scott-Alga, D Laureillar, L Weiss
    J. Infect, 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  5. Elevated Neopterin Levels Predict Early Death in Older Hip-fracture Patients
    Authors: M Larsen, C Bayard, H Lepetitcor, J Cohen-Bitt, V Appay, J Boddaert, D Sauce
    EBioMedicine, 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  6. Plasma IP-10 Concentrations Correlate Positively with Viraemia and Inversely with CD4 Counts in Untreated HIV Infection
    Authors: K Mhandire, T Mlambo, LS Zijenah, K Duri, K Mateveke, M Tshabalala, DZ Mhandire, C Musarurwa, PT Wekare, LR Mazengera, HT Matarira, B Stray-Pede
    Open AIDS J, 2017;11(0):24-31.
    Species: Human
    Sample Types: Plasma
  7. Association of Anisocytosis with Markers of Immune Activation and Exhaustion in Treated HIV
    Authors: SG Al-Kindi, DA Zidar, GA McComsey, CT Longenecke
    Pathog Immun, 2017;2(1):138-150.
    Species: Human
    Sample Types: Plasma
  8. Prospective Analysis of Lipid Composition Changes with Antiretroviral Therapy and Immune Activation in Persons Living with HIV
    Authors: MA Belury, E Bowman, J Gabriel, B Snyder, M Kulkarni, M Palettas, X Mo, JE Lake, D Zidar, SF Sieg, B Rodriguez, MP Playford, A Andrade, DR Kuritzkes, NN Mehta, MM Lederman, NT Funderburg
    Pathog Immun, 2017;2(3):376-403.
    Species: Human
    Sample Types: Plasma
  9. Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease
    Authors: YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran
    J Transl Med, 2017;15(1):121.
    Species: Human
    Sample Types: Plasma
  10. Lower Baseline Germinal Center Activity and Preserved Th1 Immunity Are Associated With Hepatitis B Vaccine Response in Treated HIV Infection
    Authors: RM Paris, LG Milagres, E Moysi, JF Okulicz, BK Agan, A Ganesan, C Petrovas, RA Koup
    Pathog Immun, 2017;2(1):66-88.
    Species: Human
    Sample Types: Plasma
  11. Quantity of alcohol drinking positively correlates with serum levels of endotoxin and markers of monocyte activation
    Authors: S Liangpunsa, E Toh, RA Ross, LE Heathers, K Chandler, A Oshodi, B McGee, E Modlik, T Linton, D Mangiacarn, C Jimenez, XC Dong, L Wang, W Tu, DE Nelson
    Sci Rep, 2017;7(1):4462.
    Species: Human
    Sample Types: Serum
  12. Changes in inflammatory biomarkers in HCV-infected patients undergoing direct acting antiviral-containing regimens with or without interferon
    Authors: C Mascia, S Vita, P Zuccalà, R Marocco, T Tieghi, S Savinelli, R Rossi, M Iannetta, I Pozzetto, C Furlan, F Mengoni, CM Mastroiann, V Vullo, M Lichtner
    PLoS ONE, 2017;12(6):e0179400.
    Species: Human
    Sample Types: Plasma
  13. Impact of probiotic Saccharomyces boulardii on the gut microbiome composition in HIV-treated patients: A double-blind, randomised, placebo-controlled trial
    Authors: J Villar-Gar, R Güerri-Fer, A Moya, A González, JJ Hernández, E Lerma, A Guelar, L Sorli, JP Horcajada, A Artacho, G D Auria, H Knobel
    PLoS ONE, 2017;12(4):e0173802.
    Species: Human
    Sample Types: Serum
  14. Impact of monotherapy on HIV-1 reservoir, immune activation, and co-infection with Epstein-Barr virus
    Authors: MR Petrara, AM Cattelan, L Sasset, R Freguja, F Carmona, S Sanavia, M Zanchetta, P Del Bianco, A De Rossi
    PLoS ONE, 2017;12(9):e0185128.
    Species: Human
    Sample Types: Plasma
  15. Reevaluation of immune activation in the era of cART and an aging HIV-infected population
    Authors: LR de Armas, S Pallikkuth, V George, S Rinaldi, R Pahwa, KL Arheart, S Pahwa
    JCI Insight, 2017;2(20):.
    Species: Human
    Sample Types: Plasma
  16. Immune Activation and Bacterial Translocation: A Link between Impaired Immune Recovery and Frequent Visceral Leishmaniasis Relapses in HIV-Infected Patients
    PLoS ONE, 2016;11(12):e0167512.
    Species: Human
    Sample Types: Plasma
  17. Bacteroides are associated with GALT iNKT cell function and reduction of microbial translocation in HIV-1 infection
    Authors: D Paquin-Pro, C Ching, I Vujkovic-C, D Fadrosh, L Loh, Y Huang, M Somsouk, SV Lynch, PW Hunt, DF Nixon, D SenGupta
    Mucosal Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
  18. P2X7 Receptor Inhibition Improves CD34 T-Cell Differentiation in HIV-Infected Immunological Nonresponders on c-ART
    Authors: I Menkova-Ga, H Hocini, E Foucat, P Tisserand, L Bourdery, C Delaugerre, C Benne, Y Lévy, JD Lelièvre
    PLoS Pathog, 2016;12(4):e1005571.
    Species: Human
    Sample Types: Plasma
  19. Cerebrospinal fluid microglia and neurodegenerative markers in twins concordant and discordant for psychotic disorders
    Authors: Viktoria Johansson
    Eur Arch Psychiatry Clin Neurosci, 2016;0(0):.
    Species: Human
    Sample Types: CSF
  20. HCV Specific IL-21 Producing T Cells but Not IL-17A Producing T Cells Are Associated with HCV Viral Control in HIV/HCV Coinfection
    PLoS ONE, 2016;11(4):e0154433.
    Species: Human
    Sample Types: Plasma
  21. Immune Activation at Sites of HIV/TB Co-Infection Contributes to the Pathogenesis of HIV-1 Disease
    PLoS ONE, 2016;11(11):e0166954.
    Species: Human
    Sample Types: Complex Sample Type
  22. Widespread activation of immunity and pro-inflammatory programs in peripheral blood leukocytes of HIV-infected patients with impaired lung gas exchange
    Authors: K Crothers, I Petrache, C Wongtrakoo, PJ Lee, LM Schnapp, SA Gharib
    Physiol Rep, 2016;4(8):.
    Species: Human
    Sample Types: Serum
  23. Antibiotic and Antiinflammatory Therapy Transiently Reduces Inflammation and Hypercoagulation in Acutely SIV-Infected Pigtailed Macaques.
    Authors: Pandrea I, Xu C, Stock J, Frank D, Ma D, Policicchio B, He T, Kristoff J, Cornell E, Haret-Richter G, Trichel A, Ribeiro R, Tracy R, Wilson C, Landay A, Apetrei C
    PLoS Pathog, 2016;12(1):e1005384.
    Species: Human
    Sample Types: Plasma
  24. Altered Monocyte and Endothelial Cell Adhesion Molecule Expression Is Linked to Vascular Inflammation in Human Immunodeficiency Virus Infection
    Authors: M Kulkarni, E Bowman, J Gabriel, T Amburgy, E Mayne, DA Zidar, C Maierhofer, AN Turner, JA Bazan, SL Koletar, MM Lederman, SF Sieg, NT Funderburg
    Open Forum Infect Dis, 2016;3(4):ofw224.
    Species: Human
    Sample Types: Plasma
  25. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization
    Authors: Valentina Cecchinato
    J. Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
  26. Immunity in young adult survivors of childhood leukemia is more similar to elderly rather than age-matched controls: Role of cytomegalovirus (CMV)
    Eur J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
  27. Increased cell-free mitochondrial DNA is a marker of ongoing inflammation and better neurocognitive function in virologically suppressed HIV-infected individuals
    Authors: Josué Pérez-Sant
    J. Neurovirol, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
  28. Microbial Translocation and Inflammation Occur in Hyperacute Immunodeficiency Virus Infection and Compromise Host Control of Virus Replication
    PLoS Pathog, 2016;12(12):e1006048.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Plasma
  29. Lack of effect of Maraviroc intensification on blood and gut reservoir
    Authors: Juan Tiraboschi
    AIDS Res. Hum. Retroviruses, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
  30. Kinetics of Myeloid-Derived Suppressor Cell Frequency and Function during Simian Immunodeficiency Virus Infection, Combination Antiretroviral Therapy, and Treatment Interruption
    Authors: Sandra E Dross
    J. Immunol, 2016;0(0):.
    Species: Rhesus macaque (Macaca mulatta)
    Sample Types: Plasma
  31. Soluble CD14 inhibits contractile function and insulin action in primary adult rat cardiomyocytes
    Authors: D Margriet Ouwens
    Biochim. Biophys. Acta, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Loss of intraepidermal nerve fiber density during SIV peripheral neuropathy is mediated by monocyte activation and elevated monocyte chemotactic proteins.
    Authors: Lakritz J, Robinson J, Polydefkis M, Miller A, Burdo T
    J Neuroinflammation, 2015;12(0):237.
    Species: Primate
    Sample Types: Plasma
  33. The immunologic effects of mesalamine in treated HIV-infected individuals with incomplete CD4+ T cell recovery: a randomized crossover trial.
    Authors: Somsouk, Ma, Dunham, Richard, Cohen, Michelle, Albright, Rebecca, Abdel-Mohsen, Mohamed, Liegler, Teri, Lifson, Jeffrey, Piatak, Michael, Gorelick, Robert, Huang, Yong, Wu, Yuaner, Hsue, Priscill, Martin, Jeffrey, Deeks, Steven G, McCune, Joseph M, Hunt, Peter W
    PLoS ONE, 2015;9(12):e116306.
    Species: Human
    Sample Types: Plasma
  34. Shift in monocyte apoptosis with increasing viral load and change in apoptosis-related ISG/Bcl2 family gene expression in chronically HIV-1-infected subjects.
    Authors: Patro S, Pal S, Bi Y, Lynn K, Mounzer K, Kostman J, Davuluri R, Montaner L
    J Virol, 2015;89(1):799-810.
    Species: Human
    Sample Types: Plasma
  35. Replicative fitness of transmitted HIV-1 drives acute immune activation, proviral load in memory CD4+ T cells, and disease progression.
    Authors: Claiborne, Daniel T, Prince, Jessica, Scully, Eileen, Macharia, Gladys, Micci, Luca, Lawson, Benton, Kopycinski, Jakub, Deymier, Martin J, Vanderford, Thomas H, Nganou-Makamdop, Krystell, Ende, Zachary, Brooks, Kelsie, Tang, Jianming, Yu, Tianwei, Lakhi, Shabir, Kilembe, William, Silvestri, Guido, Douek, Daniel, Goepfert, Paul A, Price, Matthew, Allen, Susan A, Paiardini, Mirko, Altfeld, Marcus, Gilmour, Jill, Hunter, Eric
    Proc Natl Acad Sci U S A, 2015;112(12):E1480-9.
    Species: Human
    Sample Types: Plasma
  36. Late Antiretroviral Therapy (ART) Initiation Is Associated with Long-Term Persistence of Systemic Inflammation and Metabolic Abnormalities.
    Authors: Ghislain M, Bastard J, Meyer L, Capeau J, Fellahi S, Gerard L, May T, Simon A, Vigouroux C, Goujard C
    PLoS ONE, 2015;10(12):e0144317.
    Species: Human
    Sample Types: Serum
  37. Large number of rebounding/founder HIV variants emerge from multifocal infection in lymphatic tissues after treatment interruption.
    Authors: Rothenberger, Meghan K, Keele, Brandon, Wietgrefe, Stephen, Fletcher, Courtney, Beilman, Gregory, Chipman, Jeffrey, Khoruts, Alexande, Estes, Jacob D, Anderson, Jodi, Callisto, Samuel P, Schmidt, Thomas E, Thorkelson, Ann, Reilly, Cavan, Perkey, Katherin, Reimann, Thomas G, Utay, Netanya, Nganou Makamdop, Krystell, Stevenson, Mario, Douek, Daniel C, Haase, Ashley T, Schacker, Timothy
    Proc Natl Acad Sci U S A, 2015;112(10):E1126-34.
    Species: Human
    Sample Types: Plasma
  38. Activation of NK cells is associated with HIV-1 disease progression.
    Authors: Kuri-Cervantes L, de Oca G, Avila-Rios S, Hernandez-Juan R, Reyes-Teran G
    J Leukoc Biol, 2014;96(1):7-16.
    Species: Human
    Sample Types: Plasma
  39. Pathogenic features associated with increased virulence upon Simian immunodeficiency virus cross-species transmission from natural hosts.
    Authors: Mandell D, Kristoff J, Gaufin T, Gautam R, Ma D, Sandler N, Haret-Richter G, Xu C, Aamer H, Dufour J, Trichel A, Douek D, Keele B, Apetrei C, Pandrea I
    J Virol, 2014;88(12):6778-92.
    Species: Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
    Sample Types: Plasma
  40. Reduced IgM levels and elevated IgG levels against oxidized low-density lipoproteins in HIV-1 infection.
    Authors: Yilmaz A, Jennbacken K, Fogelstrand L
    BMC Infect Dis, 2014;14(0):143.
  41. Elevated plasma soluble CD14 and skewed CD16+ monocyte distribution persist despite normalisation of soluble CD163 and CXCL10 by effective HIV therapy: a changing paradigm for routine HIV laboratory monitoring?
    Authors: Castley, Alison, Berry, Cassandr, French, Martyn, Fernandez, Sonia, Krueger, Romano, Nolan, David
    PLoS ONE, 2014;9(12):e115226.
    Species: Human
    Sample Types: Plasma
  42. Coinfection with human herpesvirus 8 is associated with persistent inflammation and immune activation in virologically suppressed HIV-infected patients.
    Authors: Masia M, Robledano C, Ortiz de la Tabla V, Antequera P, Lumbreras B, Hernandez I, Gutierrez F
    PLoS ONE, 2014;9(8):e105442.
    Species: Human
    Sample Types: Plasma
  43. Plasma sCD14 as a biomarker to predict pulmonary exacerbations in cystic fibrosis.
    Authors: Quon B, Ngan D, Wilcox P, Man S, Sin D
    PLoS ONE, 2014;9(2):e89341.
    Species: Human
    Sample Types: Plasma
  44. Factors associated with siman immunodeficiency virus transmission in a natural African nonhuman primate host in the wild.
    Authors: Ma, Dongzhu, Jasinska, Anna J, Feyertag, Felix, Wijewardana, Viskam, Kristoff, Jan, He, Tianyu, Raehtz, Kevin, Schmitt, Christop, Jung, Yoon, Cramer, Jennifer, Dione, Michel, Antonio, Martin, Tracy, Russell, Turner, Trudy, Robertson, David L, Pandrea, Ivona, Freimer, Nelson, Apetrei, Cristian
    J Virol, 2014;88(10):5687-705.
    Species: Human
    Sample Types: Plasma
  45. Progressive proximal-to-distal reduction in expression of the tight junction complex in colonic epithelium of virally-suppressed HIV+ individuals.
    Authors: Chung, Charlott, Alden, Stephani, Funderburg, Nicholas, Fu, Pingfu, Levine, Alan D
    PLoS Pathog, 2014;10(6):e1004198.
    Species: Human
    Sample Types: Plasma
  46. Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.
    Authors: Hatano, Hiroyu, Yukl, Steven A, Ferre, April L, Graf, Erin H, Somsouk, Ma, Sinclair, Elizabet, Abdel-Mohsen, Mohamed, Liegler, Teri, Harvill, Kara, Hoh, Rebecca, Palmer, Sarah, Bacchetti, Peter, Hunt, Peter W, Martin, Jeffrey, McCune, Joseph M, Tracy, Russell, Busch, Michael, O'Doherty, Una, Shacklett, Barbara, Wong, Joseph K, Deeks, Steven G
    PLoS Pathog, 2013;9(10):e1003691.
    Species: Human
    Sample Types: Plasma
  47. SIVagm infection in wild African green monkeys from South Africa: epidemiology, natural history, and evolutionary considerations.
    Authors: Ma, Dongzhu, Jasinska, Anna, Kristoff, Jan, Grobler, J Paul, Turner, Trudy, Jung, Yoon, Schmitt, Christop, Raehtz, Kevin, Feyertag, Felix, Martinez Sosa, Natalie, Wijewardana, Viskam, Burke, Donald S, Robertson, David L, Tracy, Russell, Pandrea, Ivona, Freimer, Nelson, Apetrei, Cristian
    PLoS Pathog, 2013;9(1):e1003011.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Plasma
  48. Decreased IL-7 responsiveness is related to oxidative stress in HIV disease.
    Authors: Kalinowska M, Bazdar D, Lederman M, Funderburg N, Sieg S
    PLoS ONE, 2013;8(3):e58764.
    Species: Human
    Sample Types: Serum
  49. Transcriptional effects of 1,25 dihydroxyvitamin D(3) physiological and supra-physiological concentrations in breast cancer organotypic culture.
    Authors: Milani C, Katayama M, de Lyra E, Welsh J, Campos L, Brentani M, Maciel M, Roela R, del Valle P, Goes J, Nonogaki S, Tamura R, Folgueira M
    BMC Cancer, 2013;13(0):119.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Predictive value of liver enzymes and inflammatory biomarkers for the severity of liver fibrosis stage in HIV/HCV co-infected patients.
    Authors: Charpentier C, Champenois K, Gervais A, Landman R, Joly V, Le Gac S, Larrouy L, Damond F, Brun-Vezinet F, Descamps D, Yazdanpanah Y
    PLoS ONE, 2013;8(3):e59205.
    Species: Human
    Sample Types: Plasma
  51. Dynamics of immune reconstitution and activation markers in HIV+ treatment-naive patients treated with raltegravir, tenofovir disoproxil fumarate and emtricitabine.
    Authors: Funderburg N, Andrade A, Chan E, Rosenkranz S, Lu D, Clagett B, Pilch-Cooper H, Rodriguez B, Feinberg J, Daar E, Mellors J, Kuritzkes D, Jacobson J, Lederman M
    PLoS ONE, 2013;8(12):e83514.
    Species: Human
    Sample Types: Plasma
  52. Relationship between microbial translocation and endothelial function in HIV infected patients.
    Authors: Blodget E, Shen C, Aldrovandi G, Rollie A, Gupta S, Stein J, Dube M
    PLoS ONE, 2012;7(8):e42624.
    Species: Human
    Sample Types: Plasma
  53. Shared monocyte subset phenotypes in HIV-1 infection and in uninfected subjects with acute coronary syndrome.
    Authors: Funderburg, Nicholas, Zidar, David A, Shive, Carey, Lioi, Anthony, Mudd, Joseph, Musselwhite, Laura W, Simon, Daniel I, Costa, Marco A, Rodriguez, Benigno, Sieg, Scott F, Lederman, Michael
    Blood, 2012;120(23):4599-608.
    Species: Human
    Sample Types: Plasma
  54. CD4 T follicular helper cell dynamics during SIV infection.
    J. Clin. Invest., 2012;122(9):3281-94.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Plasma
  55. Therapeutic helminth infection of macaques with idiopathic chronic diarrhea alters the inflammatory signature and mucosal microbiota of the colon.
    Authors: Broadhurst, Mara Jan, Ardeshir, Amir, Kanwar, Bittoo, Mirpuri, Julie, Gundra, Uma Mahe, Leung, Jacqueli, Wiens, Kirsten, Vujkovic-Cvijin, Ivan, Kim, Charlie, Yarovinsky, Felix, Lerche, Nicholas, McCune, Joseph M
    Oncogene, 2012;8(11):e1003000.
    Species: Human
    Sample Types: Plasma
  56. The relationship of systemic inflammation to prior hospitalization in adult patients with cystic fibrosis.
    Authors: Ngan D, Wilcox P, Aldaabil M, Li Y, Leipsic J, Sin D, Man S
    BMC Pulm Med, 2012;12(0):3.
    Species: Human
    Sample Types: Plasma
  57. Altered monocyte activation markers in Tourette's syndrome: a case-control study.
    Authors: Matz J, Krause DL, Dehning S, Riedel M, Gruber R, Schwarz MJ, Muller N
    BMC Psychiatry, 2012;12(0):29.
    Species: Human
    Sample Types: Serum
  58. Epithelial adhesion molecules can inhibit HIV-1-specific CD8+ T-cell functions.
    Authors: Streeck H, Kwon DS, Pyo A, Flanders M, Chevalier MF, Law K, Julg B, Trocha K, Jolin JS, Anahtar MN, Lian J, Toth I, Brumme Z, Chang JJ, Caron T, Rodig SJ, Milner DA, Piechoka-Trocha A, Kaufmann DE, Walker BD, Altfeld M
    Blood, 2011;117(19):5112-22.
    Species: Human
    Sample Types: Plasma
  59. Traditional risk factors and D-dimer predict incident cardiovascular disease events in chronic HIV infection.
    Authors: Ford ES, Greenwald JH, Richterman AG
    AIDS, 2010;24(10):1509-17.
    Species: Human
    Sample Types: Plasma
  60. Increased tissue factor expression on circulating monocytes in chronic HIV infection: relationship to in vivo coagulation and immune activation.
    Authors: Funderburg NT, Mayne E, Sieg SF
    Blood, 2010;115(2):161-7.
    Species: Human
    Sample Types: Plasma
  61. Maternal country of origin, breast milk characteristics and potential influences on immunity in offspring.
    Authors: Holmlund U, Amoudruz P, Johansson MA, Haileselassie Y, Ongoiba A, Kayentao K, Traore B, Doumbo S, Schollin J, Doumbo O, Montgomery SM, Sverremark-Ekstrom E
    Clin. Exp. Immunol., 2010;162(3):500-9.
    Species: Human
    Sample Types: Milk
  62. Ethnic and sex differences in circulating endotoxin levels: A novel marker of atherosclerotic and cardiovascular risk in a British multi-ethnic population.
    Authors: Miller MA, McTernan PG, Harte AL, Silva NF, Strazzullo P, Alberti KG, Kumar S, Cappuccio FP
    Atherosclerosis, 2009;203(2):494-502.
    Species: Human
    Sample Types: Plasma
  63. Cutting edge: Experimentally induced immune activation in natural hosts of simian immunodeficiency virus induces significant increases in viral replication and CD4+ T cell depletion.
    Authors: Pandrea I, Gaufin T, Brenchley JM, Gautam R, Monjure C, Gautam A, Coleman C, Lackner AA, Ribeiro RM, Douek DC, Apetrei C
    J. Immunol., 2008;181(10):6687-91.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Plasma
  64. Breast milk fatty acids may link innate and adaptive immune regulation: analysis of soluble CD14, prostaglandin E2, and fatty acids.
    Authors: Laitinen K, Hoppu U, Hamalainen M, Linderborg K, Moilanen E, Isolauri E
    Pediatr. Res., 2006;59(5):723-7.
    Species: Human
    Sample Types: Serum
  65. CD14 is an acute-phase protein.
    Authors: Bas S, Gauthier BR, Spenato U, Stingelin S, Gabay C
    J. Immunol., 2004;172(7):4470-9.
    Species: Human
    Sample Types: Serum
  66. Reduced interferon gamma production and soluble CD14 levels in early life predict recurrent wheezing by 1 year of age.
    Authors: Guerra S, Lohman IC, Halonen M, Martinez FD, Wright AL
    Am. J. Respir. Crit. Care Med., 2003;169(1):70-6.
    Species: Human
    Sample Types: Serum
  67. TGF-beta in human milk is associated with wheeze in infancy.
    Authors: Oddy WH, Halonen M, Martinez FD, Lohman IC, Stern DA, Kurzius-Spencer M, Guerra S, Wright AL
    J. Allergy Clin. Immunol., 2003;112(4):723-8.
    Species: Human
    Sample Types: Milk

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Human CD14 Quantikine ELISA Kit
By Anonymous on 10/03/2018
Sample Tested: EDTA Plasma

Human CD14 Quantikine ELISA Kit
By Anonymous on 10/03/2018
Sample Tested: EDTA Plasma

Human CD14 Quantikine ELISA Kit
By Anonymous on 03/30/2018
Sample Tested: Serum

Human CD14 Quantikine ELISA Kit
By Anonymous on 02/08/2017
Sample Tested: Human EDTA plasma

Human CD14 Quantikine ELISA Kit
By Anonymous on 12/15/2016
Sample Tested: EDTA Plasma

Plasma was isolated from blood drawn in EDTA tubes from HIV+/ART subjects and from healthy HIV- controls. sCD14 levels were measured in plasma using the Quantikine elisa Human sCD14 kit (cat# DC140). Plasma was diluted 1:200 before use in assay. Assay was carried out according to protocol provided in kit.

Figure legends:
a)Standard curve for sCD14 from which plasma sCD14 values were extrapolated.
b)Quantitated data from HIV+/ART and healthy subjects measured using the kit.

Results are expressed in ng/ml and significance calculated using unpaired Mann Whitney U-test.