Human CD14 Quantikine ELISA Kit

  (65 citations)
(3 Reviews)
    
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Product Details
Assay Procedure
Citations (65)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
  • Sensitivity
    125 pg/mL
  • Assay Range
    250.0 - 16,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human sCD14
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC20 , Quantikine Immunoassay Control Group 3 - Please Inquire
Product Summary
The Quantikine Human soluble CD14 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human soluble CD14 in cell culture supernates, serum, and plasma. It contains recombinant human CD14 expressed from CHO cells and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human sCD14 showed dose-response curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human sCD14.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 1111 2158 4187 1159 2298 4346
Standard Deviation 71 104 216 86 153 209
CV% 6.4 4.8 5.2 7.4 6.7 4.8

Recovery

The recovery of sCD14 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 91 85-105
Citrate Plasma (n=5) 102 88-110
EDTA Plasma (n=5) 98 90-107
Heparin Plasma (n=5) 96 91-104
Serum (n=5) 98 84-107
Linearity
To assess the linearity of the assay, five samples containing or spiked with high concentrations of sCD14 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 CD14 [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Note: Certificate of Analysis not available for kit components.

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Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD14
CD14 is an acute phase glycoprotein that binds lipopolysaccharide endotoxins (LPS) from Gram-negative bacteria. It is primarily expressed on monocytes, macrophages, and neutrophils, and soluble forms can be secreted or shed from the cell surface. CD14 accepts LPS from its initial binding by LBP, and it can further transfer LPS to the TLR4/MD2 complex. LPS acctivation of TLR4 triggers inflammatory activation. CD14 also contributes to the response to TLR3 ligands, phosphoinositides, as well as the surfactant proteins SP-A and SP-D.
  • Entrez Gene IDs:
    929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
  • Alternate Names:
    CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

65 Citations: Showing 1 - 10
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Species
Sample Type
  1. The CD14 (-159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes
    Authors: JJ Nieto-Font, FJ Salgado, ME San-José, MJ Cruz, A Casas-Fern, MJ Gómez-Cond, L Valdés-Cua, MÁ García-Gon, P Arias, M Nogueira, FJ González-B
    Sci Rep, 2018;8(1):4147.
    Species: Human
    Sample Type: Serum
  2. Quantity of alcohol drinking positively correlates with serum levels of endotoxin and markers of monocyte activation
    Authors: S Liangpunsa, E Toh, RA Ross, LE Heathers, K Chandler, A Oshodi, B McGee, E Modlik, T Linton, D Mangiacarn, C Jimenez, XC Dong, L Wang, W Tu, DE Nelson
    Sci Rep, 2017;7(1):4462.
    Species: Human
    Sample Type: Serum
  3. Prospective Analysis of Lipid Composition Changes with Antiretroviral Therapy and Immune Activation in Persons Living with HIV
    Authors: MA Belury, E Bowman, J Gabriel, B Snyder, M Kulkarni, M Palettas, X Mo, JE Lake, D Zidar, SF Sieg, B Rodriguez, MP Playford, A Andrade, DR Kuritzkes, NN Mehta, MM Lederman, NT Funderburg
    Pathog Immun, 2017;2(3):376-403.
    Species: Human
    Sample Type: Plasma
  4. Lower Baseline Germinal Center Activity and Preserved Th1 Immunity Are Associated With Hepatitis B Vaccine Response in Treated HIV Infection
    Authors: RM Paris, LG Milagres, E Moysi, JF Okulicz, BK Agan, A Ganesan, C Petrovas, RA Koup
    Pathog Immun, 2017;2(1):66-88.
    Species: Human
    Sample Type: Plasma
  5. Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease
    Authors: YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran
    J Transl Med, 2017;15(1):121.
    Species: Human
    Sample Type: Plasma
  6. Changes in inflammatory biomarkers in HCV-infected patients undergoing direct acting antiviral-containing regimens with or without interferon
    Authors: C Mascia, S Vita, P Zuccalà, R Marocco, T Tieghi, S Savinelli, R Rossi, M Iannetta, I Pozzetto, C Furlan, F Mengoni, CM Mastroiann, V Vullo, M Lichtner
    PLoS ONE, 2017;12(6):e0179400.
    Species: Human
    Sample Type: Plasma
  7. Association of Anisocytosis with Markers of Immune Activation and Exhaustion in Treated HIV
    Authors: SG Al-Kindi, DA Zidar, GA McComsey, CT Longenecke
    Pathog Immun, 2017;2(1):138-150.
    Species: Human
    Sample Type: Plasma
  8. Impact of monotherapy on HIV-1 reservoir, immune activation, and co-infection with Epstein-Barr virus
    Authors: MR Petrara, AM Cattelan, L Sasset, R Freguja, F Carmona, S Sanavia, M Zanchetta, P Del Bianco, A De Rossi
    PLoS ONE, 2017;12(9):e0185128.
    Species: Human
    Sample Type: Plasma
  9. Reevaluation of immune activation in the era of cART and an aging HIV-infected population
    Authors: LR de Armas, S Pallikkuth, V George, S Rinaldi, R Pahwa, KL Arheart, S Pahwa
    JCI Insight, 2017;2(20):.
    Species: Human
    Sample Type: Plasma
  10. Elevated Neopterin Levels Predict Early Death in Older Hip-fracture Patients
    Authors: M Larsen, C Bayard, H Lepetitcor, J Cohen-Bitt, V Appay, J Boddaert, D Sauce
    EBioMedicine, 2017;0(0):.
    Species: Human
    Sample Type: Plasma
  11. Fecal Bacterial Communities in treated HIV infected individuals on two antiretroviral regimens
    Authors: S Pinto-Card, C Lozupone, O Briceño, S Alva-Herná, N Téllez, A Adriana, A Murakami-O, G Reyes-Terá
    Sci Rep, 2017;7(0):43741.
    Species: Human
    Sample Type: Plasma
  12. Interleukin-1 receptor antagonist, a biomarker of response to anti-TB treatment in HIV/TB co-infected patients
    Authors: J Nouhin, P Pean, Y Madec, MF Chevalier, C Didier, L Borand, FX Blanc, D Scott-Alga, D Laureillar, L Weiss
    J. Infect, 2017;0(0):.
    Species: Human
    Sample Type: Plasma
  13. Impact of probiotic Saccharomyces boulardii on the gut microbiome composition in HIV-treated patients: A double-blind, randomised, placebo-controlled trial
    Authors: J Villar-Gar, R Güerri-Fer, A Moya, A González, JJ Hernández, E Lerma, A Guelar, L Sorli, JP Horcajada, A Artacho, G D Auria, H Knobel
    PLoS ONE, 2017;12(4):e0173802.
    Species: Human
    Sample Type: Serum
  14. Immune Activation and Bacterial Translocation: A Link between Impaired Immune Recovery and Frequent Visceral Leishmaniasis Relapses in HIV-Infected Patients
    PLoS ONE, 2016;11(12):e0167512.
    Species: Human
    Sample Type: Plasma
  15. Altered Monocyte and Endothelial Cell Adhesion Molecule Expression Is Linked to Vascular Inflammation in Human Immunodeficiency Virus Infection
    Authors: M Kulkarni, E Bowman, J Gabriel, T Amburgy, E Mayne, DA Zidar, C Maierhofer, AN Turner, JA Bazan, SL Koletar, MM Lederman, SF Sieg, NT Funderburg
    Open Forum Infect Dis, 2016;3(4):ofw224.
    Species: Human
    Sample Type: Plasma
  16. Microbial Translocation and Inflammation Occur in Hyperacute Immunodeficiency Virus Infection and Compromise Host Control of Virus Replication
    PLoS Pathog, 2016;12(12):e1006048.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Type: Plasma
  17. Immune Activation at Sites of HIV/TB Co-Infection Contributes to the Pathogenesis of HIV-1 Disease
    PLoS ONE, 2016;11(11):e0166954.
    Species: Human
    Sample Type: Complex Sample Type
  18. HCV Specific IL-21 Producing T Cells but Not IL-17A Producing T Cells Are Associated with HCV Viral Control in HIV/HCV Coinfection
    PLoS ONE, 2016;11(4):e0154433.
    Species: Human
    Sample Type: Plasma
  19. Widespread activation of immunity and pro-inflammatory programs in peripheral blood leukocytes of HIV-infected patients with impaired lung gas exchange
    Authors: K Crothers, I Petrache, C Wongtrakoo, PJ Lee, LM Schnapp, SA Gharib
    Physiol Rep, 2016;4(8):.
    Species: Human
    Sample Type: Serum
  20. Soluble CD14 inhibits contractile function and insulin action in primary adult rat cardiomyocytes
    Authors: D Margriet Ouwens
    Biochim. Biophys. Acta, 2016;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  21. Bacteroides are associated with GALT iNKT cell function and reduction of microbial translocation in HIV-1 infection
    Authors: D Paquin-Pro, C Ching, I Vujkovic-C, D Fadrosh, L Loh, Y Huang, M Somsouk, SV Lynch, PW Hunt, DF Nixon, D SenGupta
    Mucosal Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Plasma
  22. Increased cell-free mitochondrial DNA is a marker of ongoing inflammation and better neurocognitive function in virologically suppressed HIV-infected individuals
    Authors: Josué Pérez-Sant
    J. Neurovirol, 2016;0(0):.
    Species: Human
    Sample Type: Plasma
  23. Lack of effect of Maraviroc intensification on blood and gut reservoir
    Authors: Juan Tiraboschi
    AIDS Res. Hum. Retroviruses, 2016;0(0):.
    Species: Human
    Sample Type: Plasma
  24. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization
    Authors: Valentina Cecchinato
    J. Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Plasma
  25. Cerebrospinal fluid microglia and neurodegenerative markers in twins concordant and discordant for psychotic disorders
    Authors: Viktoria Johansson
    Eur Arch Psychiatry Clin Neurosci, 2016;0(0):.
    Species: Human
    Sample Type: CSF
  26. Kinetics of Myeloid-Derived Suppressor Cell Frequency and Function during Simian Immunodeficiency Virus Infection, Combination Antiretroviral Therapy, and Treatment Interruption
    Authors: Sandra E Dross
    J. Immunol, 2016;0(0):.
    Species: Rhesus macaque (Macaca mulatta)
    Sample Type: Plasma
  27. Immunity in young adult survivors of childhood leukemia is more similar to elderly rather than age-matched controls: Role of cytomegalovirus (CMV)
    Eur J Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Plasma
  28. P2X7 Receptor Inhibition Improves CD34 T-Cell Differentiation in HIV-Infected Immunological Nonresponders on c-ART
    Authors: I Menkova-Ga, H Hocini, E Foucat, P Tisserand, L Bourdery, C Delaugerre, C Benne, Y Lévy, JD Lelièvre
    PLoS Pathog, 2016;12(4):e1005571.
    Species: Human
    Sample Type: Plasma
  29. Antibiotic and Antiinflammatory Therapy Transiently Reduces Inflammation and Hypercoagulation in Acutely SIV-Infected Pigtailed Macaques.
    Authors: Pandrea I, Xu C, Stock J, Frank D, Ma D, Policicchio B, He T, Kristoff J, Cornell E, Haret-Richter G, Trichel A, Ribeiro R, Tracy R, Wilson C, Landay A, Apetrei C
    PLoS Pathog, 2016;12(1):e1005384.
    Species: Human
    Sample Type: Plasma
  30. Loss of intraepidermal nerve fiber density during SIV peripheral neuropathy is mediated by monocyte activation and elevated monocyte chemotactic proteins.
    Authors: Lakritz J, Robinson J, Polydefkis M, Miller A, Burdo T
    J Neuroinflammation, 2015;12(0):237.
    Species: Primate
    Sample Type: Plasma
  31. Late Antiretroviral Therapy (ART) Initiation Is Associated with Long-Term Persistence of Systemic Inflammation and Metabolic Abnormalities.
    Authors: Ghislain M, Bastard J, Meyer L, Capeau J, Fellahi S, Gerard L, May T, Simon A, Vigouroux C, Goujard C
    PLoS ONE, 2015;10(12):e0144317.
    Species: Human
    Sample Type: Serum
  32. Shift in monocyte apoptosis with increasing viral load and change in apoptosis-related ISG/Bcl2 family gene expression in chronically HIV-1-infected subjects.
    Authors: Patro S, Pal S, Bi Y, Lynn K, Mounzer K, Kostman J, Davuluri R, Montaner L
    J Virol, 2015;89(1):799-810.
    Species: Human
    Sample Type: Plasma
  33. The immunologic effects of mesalamine in treated HIV-infected individuals with incomplete CD4+ T cell recovery: a randomized crossover trial.
    Authors: Somsouk, Ma, Dunham, Richard, Cohen, Michelle, Albright, Rebecca, Abdel-Mohsen, Mohamed, Liegler, Teri, Lifson, Jeffrey, Piatak, Michael, Gorelick, Robert, Huang, Yong, Wu, Yuaner, Hsue, Priscill, Martin, Jeffrey, Deeks, Steven G, McCune, Joseph M, Hunt, Peter W
    PLoS ONE, 2015;9(12):e116306.
    Species: Human
    Sample Type: Plasma
  34. Replicative fitness of transmitted HIV-1 drives acute immune activation, proviral load in memory CD4+ T cells, and disease progression.
    Authors: Claiborne, Daniel T, Prince, Jessica, Scully, Eileen, Macharia, Gladys, Micci, Luca, Lawson, Benton, Kopycinski, Jakub, Deymier, Martin J, Vanderford, Thomas H, Nganou-Makamdop, Krystell, Ende, Zachary, Brooks, Kelsie, Tang, Jianming, Yu, Tianwei, Lakhi, Shabir, Kilembe, William, Silvestri, Guido, Douek, Daniel, Goepfert, Paul A, Price, Matthew, Allen, Susan A, Paiardini, Mirko, Altfeld, Marcus, Gilmour, Jill, Hunter, Eric
    Proc Natl Acad Sci U S A, 2015;112(12):E1480-9.
    Species: Human
    Sample Type: Plasma
  35. Large number of rebounding/founder HIV variants emerge from multifocal infection in lymphatic tissues after treatment interruption.
    Authors: Rothenberger, Meghan K, Keele, Brandon, Wietgrefe, Stephen, Fletcher, Courtney, Beilman, Gregory, Chipman, Jeffrey, Khoruts, Alexande, Estes, Jacob D, Anderson, Jodi, Callisto, Samuel P, Schmidt, Thomas E, Thorkelson, Ann, Reilly, Cavan, Perkey, Katherin, Reimann, Thomas G, Utay, Netanya, Nganou Makamdop, Krystell, Stevenson, Mario, Douek, Daniel C, Haase, Ashley T, Schacker, Timothy
    Proc Natl Acad Sci U S A, 2015;112(10):E1126-34.
    Species: Human
    Sample Type: Plasma
  36. Pathogenic features associated with increased virulence upon Simian immunodeficiency virus cross-species transmission from natural hosts.
    Authors: Mandell D, Kristoff J, Gaufin T, Gautam R, Ma D, Sandler N, Haret-Richter G, Xu C, Aamer H, Dufour J, Trichel A, Douek D, Keele B, Apetrei C, Pandrea I
    J Virol, 2014;88(12):6778-92.
    Species: Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
    Sample Type: Plasma
  37. Plasma sCD14 as a biomarker to predict pulmonary exacerbations in cystic fibrosis.
    Authors: Quon B, Ngan D, Wilcox P, Man S, Sin D
    PLoS ONE, 2014;9(2):e89341.
    Species: Human
    Sample Type: Plasma
  38. Elevated plasma soluble CD14 and skewed CD16+ monocyte distribution persist despite normalisation of soluble CD163 and CXCL10 by effective HIV therapy: a changing paradigm for routine HIV laboratory monitoring?
    Authors: Castley, Alison, Berry, Cassandr, French, Martyn, Fernandez, Sonia, Krueger, Romano, Nolan, David
    PLoS ONE, 2014;9(12):e115226.
    Species: Human
    Sample Type: Plasma
  39. Factors associated with siman immunodeficiency virus transmission in a natural African nonhuman primate host in the wild.
    Authors: Ma, Dongzhu, Jasinska, Anna J, Feyertag, Felix, Wijewardana, Viskam, Kristoff, Jan, He, Tianyu, Raehtz, Kevin, Schmitt, Christop, Jung, Yoon, Cramer, Jennifer, Dione, Michel, Antonio, Martin, Tracy, Russell, Turner, Trudy, Robertson, David L, Pandrea, Ivona, Freimer, Nelson, Apetrei, Cristian
    J Virol, 2014;88(10):5687-705.
    Species: Human
    Sample Type: Plasma
  40. Progressive proximal-to-distal reduction in expression of the tight junction complex in colonic epithelium of virally-suppressed HIV+ individuals.
    Authors: Chung, Charlott, Alden, Stephani, Funderburg, Nicholas, Fu, Pingfu, Levine, Alan D
    PLoS Pathog, 2014;10(6):e1004198.
    Species: Human
    Sample Type: Plasma
  41. Activation of NK cells is associated with HIV-1 disease progression.
    Authors: Kuri-Cervantes L, de Oca G, Avila-Rios S, Hernandez-Juan R, Reyes-Teran G
    J Leukoc Biol, 2014;96(1):7-16.
    Species: Human
    Sample Type: Plasma
  42. Reduced IgM levels and elevated IgG levels against oxidized low-density lipoproteins in HIV-1 infection.
    Authors: Yilmaz A, Jennbacken K, Fogelstrand L
    BMC Infect Dis, 2014;14(0):143.
  43. Coinfection with human herpesvirus 8 is associated with persistent inflammation and immune activation in virologically suppressed HIV-infected patients.
    Authors: Masia M, Robledano C, Ortiz de la Tabla V, Antequera P, Lumbreras B, Hernandez I, Gutierrez F
    PLoS ONE, 2014;9(8):e105442.
    Species: Human
    Sample Type: Plasma
  44. Transcriptional effects of 1,25 dihydroxyvitamin D(3) physiological and supra-physiological concentrations in breast cancer organotypic culture.
    Authors: Milani C, Katayama M, de Lyra E, Welsh J, Campos L, Brentani M, Maciel M, Roela R, del Valle P, Goes J, Nonogaki S, Tamura R, Folgueira M
    BMC Cancer, 2013;13(0):119.
    Species: Human
    Sample Type: Cell Culture Supernates
  45. Dynamics of immune reconstitution and activation markers in HIV+ treatment-naive patients treated with raltegravir, tenofovir disoproxil fumarate and emtricitabine.
    Authors: Funderburg N, Andrade A, Chan E, Rosenkranz S, Lu D, Clagett B, Pilch-Cooper H, Rodriguez B, Feinberg J, Daar E, Mellors J, Kuritzkes D, Jacobson J, Lederman M
    PLoS ONE, 2013;8(12):e83514.
    Species: Human
    Sample Type: Plasma
  46. Prospective antiretroviral treatment of asymptomatic, HIV-1 infected controllers.
    Authors: Hatano, Hiroyu, Yukl, Steven A, Ferre, April L, Graf, Erin H, Somsouk, Ma, Sinclair, Elizabet, Abdel-Mohsen, Mohamed, Liegler, Teri, Harvill, Kara, Hoh, Rebecca, Palmer, Sarah, Bacchetti, Peter, Hunt, Peter W, Martin, Jeffrey, McCune, Joseph M, Tracy, Russell, Busch, Michael, O'Doherty, Una, Shacklett, Barbara, Wong, Joseph K, Deeks, Steven G
    PLoS Pathog, 2013;9(10):e1003691.
    Species: Human
    Sample Type: Plasma
  47. Predictive value of liver enzymes and inflammatory biomarkers for the severity of liver fibrosis stage in HIV/HCV co-infected patients.
    Authors: Charpentier C, Champenois K, Gervais A, Landman R, Joly V, Le Gac S, Larrouy L, Damond F, Brun-Vezinet F, Descamps D, Yazdanpanah Y
    PLoS ONE, 2013;8(3):e59205.
    Species: Human
    Sample Type: Plasma
  48. SIVagm infection in wild African green monkeys from South Africa: epidemiology, natural history, and evolutionary considerations.
    Authors: Ma, Dongzhu, Jasinska, Anna, Kristoff, Jan, Grobler, J Paul, Turner, Trudy, Jung, Yoon, Schmitt, Christop, Raehtz, Kevin, Feyertag, Felix, Martinez Sosa, Natalie, Wijewardana, Viskam, Burke, Donald S, Robertson, David L, Tracy, Russell, Pandrea, Ivona, Freimer, Nelson, Apetrei, Cristian
    PLoS Pathog, 2013;9(1):e1003011.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Type: Plasma
  49. Decreased IL-7 responsiveness is related to oxidative stress in HIV disease.
    Authors: Kalinowska M, Bazdar D, Lederman M, Funderburg N, Sieg S
    PLoS ONE, 2013;8(3):e58764.
    Species: Human
    Sample Type: Serum
  50. Shared monocyte subset phenotypes in HIV-1 infection and in uninfected subjects with acute coronary syndrome.
    Authors: Funderburg, Nicholas, Zidar, David A, Shive, Carey, Lioi, Anthony, Mudd, Joseph, Musselwhite, Laura W, Simon, Daniel I, Costa, Marco A, Rodriguez, Benigno, Sieg, Scott F, Lederman, Michael
    Blood, 2012;120(23):4599-608.
    Species: Human
    Sample Type: Plasma
  51. Therapeutic helminth infection of macaques with idiopathic chronic diarrhea alters the inflammatory signature and mucosal microbiota of the colon.
    Authors: Broadhurst, Mara Jan, Ardeshir, Amir, Kanwar, Bittoo, Mirpuri, Julie, Gundra, Uma Mahe, Leung, Jacqueli, Wiens, Kirsten, Vujkovic-Cvijin, Ivan, Kim, Charlie, Yarovinsky, Felix, Lerche, Nicholas, McCune, Joseph M
    Oncogene, 2012;8(11):e1003000.
    Species: Human
    Sample Type: Plasma
  52. Relationship between microbial translocation and endothelial function in HIV infected patients.
    Authors: Blodget E, Shen C, Aldrovandi G, Rollie A, Gupta S, Stein J, Dube M
    PLoS ONE, 2012;7(8):e42624.
    Species: Human
    Sample Type: Plasma
  53. Altered monocyte activation markers in Tourette's syndrome: a case-control study.
    Authors: Matz J, Krause DL, Dehning S, Riedel M, Gruber R, Schwarz MJ, Muller N
    BMC Psychiatry, 2012;12(0):29.
    Species: Human
    Sample Type: Serum
  54. The relationship of systemic inflammation to prior hospitalization in adult patients with cystic fibrosis.
    Authors: Ngan D, Wilcox P, Aldaabil M, Li Y, Leipsic J, Sin D, Man S
    BMC Pulm Med, 2012;12(0):3.
    Species: Human
    Sample Type: Plasma
  55. CD4 T follicular helper cell dynamics during SIV infection.
    J. Clin. Invest., 2012;122(9):3281-94.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Plasma
  56. Epithelial adhesion molecules can inhibit HIV-1-specific CD8+ T-cell functions.
    Authors: Streeck H, Kwon DS, Pyo A, Flanders M, Chevalier MF, Law K, Julg B, Trocha K, Jolin JS, Anahtar MN, Lian J, Toth I, Brumme Z, Chang JJ, Caron T, Rodig SJ, Milner DA, Piechoka-Trocha A, Kaufmann DE, Walker BD, Altfeld M
    Blood, 2011;117(19):5112-22.
    Species: Human
    Sample Type: Plasma
  57. Increased tissue factor expression on circulating monocytes in chronic HIV infection: relationship to in vivo coagulation and immune activation.
    Authors: Funderburg NT, Mayne E, Sieg SF
    Blood, 2010;115(2):161-7.
    Species: Human
    Sample Type: Plasma
  58. Maternal country of origin, breast milk characteristics and potential influences on immunity in offspring.
    Authors: Holmlund U, Amoudruz P, Johansson MA, Haileselassie Y, Ongoiba A, Kayentao K, Traore B, Doumbo S, Schollin J, Doumbo O, Montgomery SM, Sverremark-Ekstrom E
    Clin. Exp. Immunol., 2010;162(3):500-9.
    Species: Human
    Sample Type: Milk
  59. Traditional risk factors and D-dimer predict incident cardiovascular disease events in chronic HIV infection.
    Authors: Ford ES, Greenwald JH, Richterman AG
    AIDS, 2010;24(10):1509-17.
    Species: Human
    Sample Type: Plasma
  60. Ethnic and sex differences in circulating endotoxin levels: A novel marker of atherosclerotic and cardiovascular risk in a British multi-ethnic population.
    Authors: Miller MA, McTernan PG, Harte AL, Silva NF, Strazzullo P, Alberti KG, Kumar S, Cappuccio FP
    Atherosclerosis, 2009;203(2):494-502.
    Species: Human
    Sample Type: Plasma
  61. Cutting edge: Experimentally induced immune activation in natural hosts of simian immunodeficiency virus induces significant increases in viral replication and CD4+ T cell depletion.
    Authors: Pandrea I, Gaufin T, Brenchley JM, Gautam R, Monjure C, Gautam A, Coleman C, Lackner AA, Ribeiro RM, Douek DC, Apetrei C
    J. Immunol., 2008;181(10):6687-91.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Type: Plasma
  62. Breast milk fatty acids may link innate and adaptive immune regulation: analysis of soluble CD14, prostaglandin E2, and fatty acids.
    Authors: Laitinen K, Hoppu U, Hamalainen M, Linderborg K, Moilanen E, Isolauri E
    Pediatr. Res., 2006;59(5):723-7.
    Species: Human
    Sample Type: Serum
  63. CD14 is an acute-phase protein.
    Authors: Bas S, Gauthier BR, Spenato U, Stingelin S, Gabay C
    J. Immunol., 2004;172(7):4470-9.
    Species: Human
    Sample Type: Serum
  64. Reduced interferon gamma production and soluble CD14 levels in early life predict recurrent wheezing by 1 year of age.
    Authors: Guerra S, Lohman IC, Halonen M, Martinez FD, Wright AL
    Am. J. Respir. Crit. Care Med., 2003;169(1):70-6.
    Species: Human
    Sample Type: Serum
  65. TGF-beta in human milk is associated with wheeze in infancy.
    Authors: Oddy WH, Halonen M, Martinez FD, Lohman IC, Stern DA, Kurzius-Spencer M, Guerra S, Wright AL
    J. Allergy Clin. Immunol., 2003;112(4):723-8.
    Species: Human
    Sample Type: Milk
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Average Rating: 4 (Based on 3 reviews)

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  Very Good
  Anonymous 03/30/2018
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Summary

Sample Tested Serum
 Human CD14 Quantikine ELISA Kit DC140 Array DC140
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Very Good
  Anonymous 02/08/2017
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 Human CD14 Quantikine ELISA Kit DC140
: Human CD14 Quantikine ELISA Kit [DC140].

Summary

Sample Tested Human EDTA plasma
 Human CD14 Quantikine ELISA Kit DC140 Array DC140
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Very Good
  Anonymous 12/15/2016
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 Human CD14 Quantikine ELISA Kit DC140
: Human CD14 Quantikine ELISA Kit [DC140].

Summary

Sample Tested EDTA Plasma

Other Experimental Details

Other Experimental Details Plasma was isolated from blood drawn in EDTA tubes from HIV+/ART subjects and from healthy HIV- controls. sCD14 levels were measured in plasma using the Quantikine elisa Human sCD14 kit (cat# DC140). Plasma was diluted 1:200 before use in assay. Assay was carried out according to protocol provided in kit.Figure legends:a)Standard curve for sCD14 from which plasma sCD14 values were extrapolated.b)Quantitated data from HIV+/ART and healthy subjects measured using the kit. Results are expressed in ng/ml and significance calculated using unpaired Mann Whitney U-test.

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