Human CD24 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD24 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD24a is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human CD24
    Ser27-Gly59
    Accession # NP_037362
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human colon cancer tissue and lymph node tissue
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CD24 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5247) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD24 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD24

CD24 (signal transducer 24; also HSA/heat shock antigen) is a variably glycosylated, 35‑50 kDa member of the CD24 family of proteins. It is a GPI‑linked sialoprotein that is expressed on B cell precursors and unactivated B cells, keratinocytes, and neutrophils and thymocytes. Ligation of CD24 appears to inhibit B cell differentiation, likely through the initiation of apoptosis. Potential CD24 ligands include P‑Selectin. The human CD24 precursor is 80 amino acids (aa) in length. It contains a 26 aa signal sequence plus a cleavable C‑terminal propeptide (aa 60‑80). There is one potential alternate start site at Met5. Over aa 27‑59, human CD24 shares only 30% aa identity with mouse CD24.

  • Entrez Gene IDs:
    100133941 (Human); 12484 (Mouse)
  • Alternate Names:
    CD 24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24; CD24A; CD24Asignal transducer CD24; FLJ22950; FLJ43543; MGC75043; Small cell lung carcinoma cluster 4 antigen
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