Detection of Human CD24 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5247) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD24 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD24 (signal transducer 24; also HSA/heat shock antigen) is a variably glycosylated, 35‑50 kDa member of the CD24 family of proteins. It is a GPI‑linked sialoprotein that is expressed on B cell precursors and unactivated B cells, keratinocytes, and neutrophils and thymocytes. Ligation of CD24 appears to inhibit B cell differentiation, likely through the initiation of apoptosis. Potential CD24 ligands include P‑Selectin. The human CD24 precursor is 80 amino acids (aa) in length. It contains a 26 aa signal sequence plus a cleavable C‑terminal propeptide (aa 60‑80). There is one potential alternate start site at Met5. Over aa 27‑59, human CD24 shares only 30% aa identity with mouse CD24.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.