Human CD24 Antibody

Catalog # Availability Size / Price Qty
Detection of Human CD24 by Western Blot.
1 Image
Product Details
Citations (4)
Supplemental Products

Human CD24 Antibody Summary

Species Reactivity
Detects human CD24 in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant human CD24
Accession # NP_037362
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human colon cancer tissue and lymph node tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD24 antibody by Western Blot. View Larger

Detection of Human CD24 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5247) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD24 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD24

CD24 (signal transducer 24; also HSA/heat shock antigen) is a variably glycosylated, 35‑50 kDa member of the CD24 family of proteins. It is a GPI‑linked sialoprotein that is expressed on B cell precursors and unactivated B cells, keratinocytes, and neutrophils and thymocytes. Ligation of CD24 appears to inhibit B cell differentiation, likely through the initiation of apoptosis. Potential CD24 ligands include P‑Selectin. The human CD24 precursor is 80 amino acids (aa) in length. It contains a 26 aa signal sequence plus a cleavable C‑terminal propeptide (aa 60‑80). There is one potential alternate start site at Met5. Over aa 27‑59, human CD24 shares only 30% aa identity with mouse CD24.

Entrez Gene IDs
100133941 (Human); 12484 (Mouse)
Alternate Names
CD 24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24; CD24A; CD24Asignal transducer CD24; FLJ22950; FLJ43543; MGC75043; Small cell lung carcinoma cluster 4 antigen

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Citations for Human CD24 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. A pilot study on biological characteristics of human CD24(+) stem cells from the apical papilla
    Authors: J Liang, YJ Zhao, JQ Li, L Lan, WJ Tao, JY Wu
    Journal of dental sciences, 2022;17(1):264-275.
    Species: Human
    Sample Types: Whole Tissue
    Applications: ICC
  2. Arsenic Promotes the COX2/PGE2-SOX2 Axis to Increase the Malignant Stemness Properties of Urothelial Cells
    Authors: A Ooki, A Begum, L Marchionni, CJ VandenBuss, S Mao, K Max, MO Hoque
    Int. J. Cancer, 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. CD24 regulates cancer stem cell (CSC)-like traits and a panel of CSC-related molecules serves as a non-invasive urinary biomarker for the detection of bladder cancer
    Authors: A Ooki, CJ VandenBuss, M Kates, NM Hahn, A Matoso, DJ McConkey, TJ Bivalacqua, MO Hoque
    Br. J. Cancer, 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. PMP22 Regulates Self-Renewal and Chemoresistance of Gastric Cancer Cells
    Authors: W Cai, G Chen, Q Luo, J Liu, X Guo, T Zhang, F Ma, L Yuan, B Li, J Cai
    Mol. Cancer Ther, 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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