Human CD25/IL-2R alpha (Research Grade Basiliximab Biosimilar) Antibody

R&D Systems | Catalog # MAB9926

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu107
View all CD25/IL-2R alpha Primary Antibodies »

Product Specifications

Immunogen

Human CD25/IL-2 R alpha

Specificity

Detects Human CD25/IL-2 R alpha based on Basiliximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Basiliximab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images

IL-2 binding to Human CD25/IL-2R alpha-transfected HEK293 Human Cell Line is Blocked by Human CD25/IL-2R alpha Antibody.

IL-2 binding to Human CD25/IL-2R alpha-transfected HEK293 Human Cell Line is Blocked by Human CD25/IL-2R alpha Antibody.

In a functional flow cytometry test, Recombinant Human IL-2 Biotinylated protein (Catalog # BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 µg/mL of Human Anti-Human CD25/IL-2R alpha (Research Grade Basiliximab Biosimilar) Monoclonal Antibody (Catalog # MAB9926). Human IgG Isotype Control (Catalog # 1-001-A) at 0.5 µg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050).
Detection of CD25/IL-2 R alpha antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry.

Human PBMCs were treated with PHA (5 µg/mL for 48hr) then stained with (A) Human CD25/IL-2 R alpha (Research Grade Basiliximab Biosimilar) Monoclonal Antibody (Catalog # MAB9926) or (B) Normal Human IgG control antibody (Catalog # 1-001-A), followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antiobdy (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Applications

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

0.5 µg/106 cells
Sample: In a functional flow cytometry test, 0.5 μg/mL of Human Anti-Human CD25/IL-2R alpha Antibody(Catalog # MAB9926) will block the binding of 500 ng/mL Biotinylated Recombinant Human IL-2 (Catalog # BT202) to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMCs treated with PHA

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD25/IL-2R alpha

Basiliximab is a biosimilar directed against the extracellular domain of CD25/IL-2 R alpha. This product an be used in flow cytometry or various assay format to measure CD25/IL-2R alpha expression.

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Gene Symbol

IL2RA

Additional CD25/IL-2R alpha Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices

For research use only

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FAQs

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    A: The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  • Q: Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    A: The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

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