Human CD25/IL-2R alpha (Research Grade Daclizumab Biosimilar) Antibody
Human CD25/IL-2R alpha (Research Grade Daclizumab Biosimilar) Antibody Summary
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry. Human PBMCs were treated with PHA (5 µg/mL for 24hr) then stained with (A) Human CD25/IL-2 Ra (Daclizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9927) or (B) Normal Human IgG control antibody (Catalog # 1-001-A), followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antiobdy (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD25/IL-2R alpha
Daclizumab is a biosimilar directed against the extracellular domain of CD25/IL-2 R alpha. This product can also be used in flow cytometry or various assay formats to measure CD25/IL-2 R alpha.
Citation for Human CD25/IL-2R alpha (Research Grade Daclizumab Biosimilar) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Antibody-guided design and identification of CD25-binding small antibody mimetics using mammalian cell surface display
Authors: K See, T Kadonosono, K Miyamoto, T Tsubaki, Y Ota, M Katsumi, S Ryo, K Aida, M Minegishi, T Isozaki, T Kuchimaru, S Kizaka-Kon
Scientific Reports, 2021;11(1):22098.
Sample Types: Recombinant Proteins
Applications: Functional Assay
Does this Biosimilar antibody have the same sequence as the therapeutic antibody?
The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody.
What is a Biosimilar antibody?
A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody. It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody. For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.
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