Human CD34 Alexa Fluor® 700-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD34 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD45 PE-conjugated Monoclonal Antibody (Catalog # FAB1430P) and either (A) Mouse Anti-Human CD34 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB7227N) or (B) Mouse IgG1 Alexa Fluor 700 Isotype Control (Catalog # IC002N). View our protocol for Staining Membrane-associated Proteins.
Detection of CD34 in KG‑1a Human Cell Line by Flow Cytometry. KG-1a human acute myelogenous leukemia cell line was stained with Mouse Anti-Human CD34 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB7227N, filled histogram) or isotype control antibody (IC002N, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.
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