Human CD34 Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7227N-100UG
Detection of CD34 in Human PBMCs by Flow Cytometry.
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Human CD34 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD34 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # QBEnd10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human endothelial vesicles
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CD34 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD34 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD45 PE-conjugated Monoclonal Antibody (Catalog # FAB1430P) and either (A) Mouse Anti-Human CD34 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB7227N) or (B) Mouse IgG1 Alexa Fluor 700 Isotype Control (Catalog # IC002N). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of CD34 in KG‑1a Human Cell Line by Flow Cytometry. KG-1a human acute myelogenous leukemia cell line was stained with Mouse Anti-Human CD34 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB7227N, filled histogram) or isotype control antibody (IC002N, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitution default not known
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD34

The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

Entrez Gene IDs
947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
Alternate Names
CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human CD34 Alexa Fluor® 700-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human CD34 Alexa Fluor® 700-conjugated Antibody and earn rewards!

Have you used Human CD34 Alexa Fluor® 700-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review