Human CD34 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7227A
FAB7227A-025
Detection  of CD34 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (2)
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Human CD34 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD34 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # QBEnd10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human endothelial vesicles
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection  of CD34 in Human PBMCs by Flow Cytometry. View Larger

Detection of CD34 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD34 APC‑conjugated Monoclonal Antibody (Catalog # FAB7227A) and Mouse Anti-Human CD45 PE‑conjugated Monoclonal Antibody (Catalog # FAB1430P). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins

Flow Cytometry Detection of CD34 in KG‑1a Human Cell Line by Flow Cytometry. View Larger

Detection of CD34 in KG‑1a Human Cell Line by Flow Cytometry. KG‑1a human acute myelogenous leukemia cell line was stained with Mouse Anti-Human CD34 APC‑conjugated Monoclonal Antibody (Catalog # FAB7227A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD34

The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

Entrez Gene IDs
947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
Alternate Names
CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1

Product Datasheets

Citations for Human CD34 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A Versatile Safeguard for Chimeric Antigen Receptor T-Cell Immunotherapies
    Authors: J Valton, V Guyot, B Boldajipou, C Sommer, T Pertel, A Juillerat, A Duclert, BJ Sasu, P Duchateau, L Poirot
    Sci Rep, 2018;8(1):8972.
    Species: Human
    Sample Types: Whole Cells
  2. Chimeric Antigen Receptor-Engineered Human Gamma Delta T Cells: Enhanced Cytotoxicity with Retention of Cross Presentation
    Authors: A Capsomidis, G Benthall, HH Van Acker, J Fisher, AM Kramer, Z Abeln, Y Majani, T Gileadi, R Wallace, K Gustafsson, B Flutter, J Anderson
    Mol. Ther., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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