Human CD34 APC-conjugated Antibody

  (1 citations)     
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Product Details
Citations (1)
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  • Species Reactivity
    Human
  • Specificity
    Detects human CD34 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # QBEnd10
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human endothelial vesicles
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD34 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD34 APC‑conjugated Monoclonal Antibody (Catalog # FAB7227A) and Mouse Anti-Human CD45 PE‑conjugated Monoclonal Antibody (Catalog # FAB1430P). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins
Detection of CD34 in KG‑1a Human Cell Line by Flow Cytometry. KG‑1a human acute myelogenous leukemia cell line was stained with Mouse Anti-Human CD34 APC‑conjugated Monoclonal Antibody (Catalog # FAB7227A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD34
The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.
  • Entrez Gene IDs:
    947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
  • Alternate Names:
    CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Chimeric Antigen Receptor-Engineered Human Gamma Delta T Cells: Enhanced Cytotoxicity with Retention of Cross Presentation
    Authors: A Capsomidis, G Benthall, HH Van Acker, J Fisher, AM Kramer, Z Abeln, Y Majani, T Gileadi, R Wallace, K Gustafsson, B Flutter, J Anderson
    Mol. Ther., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 11  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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