Human CD34 APC-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD34 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD34 APC‑conjugated Monoclonal Antibody (Catalog # FAB7227A) and Mouse Anti-Human CD45 PE‑conjugated Monoclonal Antibody (Catalog # FAB1430P). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Detection of CD34 in KG‑1a Human Cell Line by Flow Cytometry. KG‑1a human acute myelogenous leukemia cell line was stained with Mouse Anti-Human CD34 APC‑conjugated Monoclonal Antibody (Catalog # FAB7227A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.
Citations for Human CD34 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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A Versatile Safeguard for Chimeric Antigen Receptor T-Cell Immunotherapies
Authors: J Valton, V Guyot, B Boldajipou, C Sommer, T Pertel, A Juillerat, A Duclert, BJ Sasu, P Duchateau, L Poirot
Sci Rep, 2018;8(1):8972.
Sample Types: Whole Cells
Chimeric Antigen Receptor-Engineered Human Gamma Delta T Cells: Enhanced Cytotoxicity with Retention of Cross Presentation
Authors: A Capsomidis, G Benthall, HH Van Acker, J Fisher, AM Kramer, Z Abeln, Y Majani, T Gileadi, R Wallace, K Gustafsson, B Flutter, J Anderson
Mol. Ther., 2017;0(0):.
Sample Types: Whole Cells
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