Human CD34 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7227G
Detection of CD34 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (6)
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Human CD34 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD34 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # QBEnd10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human endothelial vesicles
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below
Immunocytochemistry
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD34 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD34 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD45 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB1430N) and either (A) Mouse Anti-Human CD34 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7227G) or (B) Mouse IgG1Alexa Fluor 488 Isotype Control (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry CD34 antibody in KG-1 Human Cell Line by Immunocytochemistry (ICC). View Larger

CD34 in KG-1 Human Cell Line. CD34 was detected in immersion fixed KG-1 human acute myelogenous leukemia cell line using Mouse Anti-Human CD34 AlexaFluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7227G, green) at 10 μg/mL for 3 hours at room temperature and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow Cytometry View Larger

Detection of CD34 in KG‑1a Human Cell Line by Flow Cytometry. KG-1a human acute myelogenous leukemia cell line was stained with Mouse Anti-Human CD34 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7227G, filled histogram) or isotype control antibody (IC002G, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD34

The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein Kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

Entrez Gene IDs
947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
Alternate Names
CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human CD34 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Enhancing CAR T-cell Therapy Using Fab-Based Constitutively Heterodimeric Cytokine Receptors
    Authors: Matteo Righi, Isaac Gannon, Matthew Robson, Saket Srivastava, Evangelia Kokalaki, Thomas Grothier et al.
    Cancer Immunology Research
  2. Augmenting the Angiogenic Profile and Functionality of Cord Blood Endothelial Colony-Forming Cells by Indirect Priming with Bone-Marrow-Derived Mesenchymal Stromal Cells
    Authors: Bansal, A;Singh, A;Nag, TC;Sharma, D;Garg, B;Bhatla, N;Choudhury, SD;Ramakrishnan, L;
    Biomedicines
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Development of a TGFbeta-IL-2/15 Switch Receptor for Use in Adoptive Cell Therapy
    Authors: C Beck, NP Casey, I Persiconi, NN Moharrami, A Sike, Y Jin, JA Kyte
    Biomedicines, 2023-02-04;11(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Role of endothelial colony forming cells (ECFCs) Tetrahydrobiopterin (BH4) in determining ECFCs functionality in coronary artery disease (CAD) patients
    Authors: A Sen, A Singh, A Roy, S Mohanty, N Naik, M Kalaivani, L Ramakrishn
    Scientific Reports, 2022-02-23;12(1):3076.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Repurposing endogenous immune pathways to tailor and control chimeric antigen receptor T�cell functionality
    Authors: M Sachdeva, BW Busser, S Temburni, B Jahangiri, AS Gautron, A Maréchal, A Juillerat, A Williams, S Depil, P Duchateau, L Poirot, J Valton
    Nat Commun, 2019-11-13;10(1):5100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Aerobic exercise in humans mobilizes HSC in an intensity dependent manner
    Authors: Gianni Parise
    J. Appl. Physiol., 2016-11-23;0(0):jap.00696.201.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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