Human CD34 Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human CD34 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # QBEnd10
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human endothelial vesicles
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
  • Immunocytochemistry
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD34 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD45 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB1430N) and either (A) Mouse Anti-Human CD34 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7227G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins.
CD34 in KG-1 Human Cell Line. CD34 was detected in immersion fixed KG-1 human acute myelogenous leukemia cell line using Mouse Anti-Human CD34 AlexaFluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7227G, green) at 10 μg/mL for 3 hours at room temperature and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD34
The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein Kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.
  • Entrez Gene IDs:
    947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
  • Alternate Names:
    CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

Ctrl IC002G 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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