Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 498403
View all CD39/ENTPD1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD39/ENTPD1
Thr38-Val478
Accession # P49961

Specificity

Detects human CD39/ENTPD1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD39, recombinant human CD39L2, 3, or 4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD39/ENTPD1 Antibody

Detection of CD39/ENTPD1 antibody in Human Monocytes antibody by Flow Cytometry.

Detection of CD39/ENTPD1 in Human Monocytes by Flow Cytometry.

Human whole blood monocytes were stained with Mouse Anti-Human CD39/ENTPD1 Mono-clonal Antibody (Catalog # MAB4397, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phy-coerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Applications for Human CD39/ENTPD1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood monocytes

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD39/ENTPD1

Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. rhNTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble rhNTPDase-1 ectodomain. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (2), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (3). NTPDase-1 hydrolyzes the beta - and gamma  phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1 plays a role in the regulation of purinergic signaling (4). NTPDase-1 is involved in the processes of thrombo regulation and vascular inflammation (5). The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (6).

References

  1. Maliszewski, C.R. et al. (1994) J. Immunol. 153:3574.
  2. Rowe, M. et al. (1982) Int. J. Cancer 29:373.
  3. Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
  4. Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
  5. Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
  6. Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.

Long Name

Ecto-Nucleoside Triphosphate Diphosphohydrolase 1

Alternate Names

ADPase, ATP-diphosphatase, CD39, ENTPD1, NTPD1

Entrez Gene IDs

953 (Human); 12495 (Mouse); 64519 (Rat); 102132860 (Cynomolgus Monkey)

Gene Symbol

ENTPD1

UniProt

P49961

Additional CD39/ENTPD1 Products

Product Documents for Human CD39/ENTPD1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD39/ENTPD1 Antibody

For research use only

Citations for Human CD39/ENTPD1 Antibody

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