Detects human CD40/TNFRSF5 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rhRANK, rhTAJ, rhTNF RI, rhTNF RII, or recombinant mouse CD40.
Monoclonal Mouse IgG2B Clone # 82111
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human CD40/TNFRSF5 Glu21-Arg193 Accession # P25942
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD40/TNFRSF5 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD40/TNFRSF5 PE‑conjugated Monoclonal Antibody (Catalog # FAB6321P) and Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F). Quadrant markers were set based on control antibody staining (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD40, also known as TNFRSF5, is a 45-50 kD type I transmembrane
glycoprotein that belongs to the TNF receptor superfamily. Mature human CD40 is
257 amino acids (aa) in length, and consists of a 173 aa extracellular domain
(ECD), a 22 aa transmembrane segment and a 62 aa cytoplasmic region. The ECD
contains four cysteine-rich repeats, the second of which is considered crucial
to ligand binding. Over aa 21-193, human
CD40 shares 58% aa sequence identity with mouse CD40. Although not ubiquitously expressed, cells known
to constitutively or inducibly express CD40 are large in number and include
basal layer keratinocytes, adipocytes, CD8+ T cells, fibrocytes (circulating
stem cells), monocytes, eosinophils, dendritic cells, pancreatic duct
epithelium, astrocytes, smooth muscle cells, fibroblasts, vascular endothelial
cells and B cells. There is at least one
alternative splice form that shows a 38 aa substitution for aa 166-277,
generating a soluble molecule. There are
two "ligands" for CD40, the first being CD154/CD40L, and the second Borrelia, a
spirochete that causes Lyme disease.
CD40 ligation by CD154 has many outcomes, some of which are
cell-specific. Ligation of CD40 on B
cells (with IL-4) induces IgE production, while bone marrow stromal cell
activation via CD40 promotes OPG secretion with concomitant bone
formation. In addition, stimulation of
CD40 on keratinocytes results in IL-8 and RANTES expression with subsequent
leukocyte infiltration, while adipocyte activation via CD40 promotes local M1
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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