Human CD40/TNFRSF5 PE-conjugated Antibody

  (2 citations)     
Datasheet / CoA / SDS
Product Details
Citations (2)
Supplemental Products
  • Species Reactivity
  • Specificity
    Detects human CD40/TNFRSF5 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rhRANK, rhTAJ, rhTNF RI, rhTNF RII, or recombinant mouse CD40.
  • Source
    Monoclonal Mouse IgG2B Clone # 82111
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD40/TNFRSF5
    Accession # P25942
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
Product Datasheets

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  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD40/TNFRSF5 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD40/TNFRSF5 PE‑conjugated Monoclonal Antibody (Catalog # FAB6321P) and Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F). Quadrant markers were set based on control antibody staining (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD40/TNFRSF5
CD40, also known as TNFRSF5, is a 45-50 kD type I transmembrane glycoprotein that belongs to the TNF receptor superfamily. Mature human CD40 is 257 amino acids (aa) in length, and consists of a 173 aa extracellular domain (ECD), a 22 aa transmembrane segment and a 62 aa cytoplasmic region. The ECD contains four cysteine-rich repeats, the second of which is considered crucial to ligand binding. Over aa 21-193, human CD40 shares 58% aa sequence identity with mouse CD40. Although not ubiquitously expressed, cells known to constitutively or inducibly express CD40 are large in number and include basal layer keratinocytes, adipocytes, CD8+ T cells, fibrocytes (circulating stem cells), monocytes, eosinophils, dendritic cells, pancreatic duct epithelium, astrocytes, smooth muscle cells, fibroblasts, vascular endothelial cells and B cells. There is at least one alternative splice form that shows a 38 aa substitution for aa 166-277, generating a soluble molecule. There are two "ligands" for CD40, the first being CD154/CD40L, and the second Borrelia, a spirochete that causes Lyme disease. CD40 ligation by CD154 has many outcomes, some of which are cell-specific. Ligation of CD40 on B cells (with IL-4) induces IgE production, while bone marrow stromal cell activation via CD40 promotes OPG secretion with concomitant bone formation. In addition, stimulation of CD40 on keratinocytes results in IL-8 and RANTES expression with subsequent leukocyte infiltration, while adipocyte activation via CD40 promotes local M1 polarization.
  • Entrez Gene IDs:
    958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)
  • Alternate Names:
    B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Sample Type
  1. High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
    Authors: Hennig C, Ilginus C, Boztug K, Skokowa J, Marodi L, Szaflarska A, Sass M, Pignata C, Kilic S, Caragol I, Baumann U, Klein C, Welte K, Hansen G
    J Allergy Clin Immunol, 2014;133(1):172-80.e1-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Chip Cytometry
  2. Oxidation of ovarian epithelial cancer cells by hypochlorous acid enhances immunogenicity and stimulates T cells that recognize autologous primary tumor.
    Authors: Chiang CL, Ledermann JA, Aitkens E, Benjamin E, Katz DR, Chain BM
    Clin. Cancer Res., 2008;14(15):4898-907.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow


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Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 24  
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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